Article
Microbiology
Amol M. Sawant, Vishwambar D. Navale, Koteswara Rao Vamkudoth
Summary: In this study, 80 strains of Penicillium chrysogenum/rubens were screened for PenV production, with 28 strains capable of producing PenV. By monitoring and optimizing fermentation parameters, the production of PenV can be further improved.
Article
Infectious Diseases
Li-Na Guo, Shu-Ying Yu, Yao Wang, Ya-Li Liu, Ying Yuan, Si-Meng Duan, Wen-Hang Yang, Xin-Miao Jia, Ying Zhao, Meng Xiao, Xiu-Li Xie, Hong-Tao Dou, Po-Ren Hsueh, Ying-Chun Xu
Summary: Morphologically identified Penicillium and Talaromyces marneffei isolates were collected from clinical sources, and species were confirmed using sequencing. Most Penicillium isolates were Penicillium oxalicum, while Talaromyces isolates were mainly Talaromyces funiculosus and Talaromyces stollii. Antifungal susceptibility varied among species, with echinocandins showing the lowest minimum effective concentrations for most species.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Microbiology
Li-An Wang, Yu-Chuan Chuang, Ting-Kuang Yeh, Kuan-Pei Lin, Chi-Jan Lin, Po-Yu Liu
Summary: This is the first reported case in Taiwan of an AIDS patient with concurrent Cryptococcus neoformans meningitis and Talaromyces amestolkiae lymphadenopathy. DNA sequencing was used to differentiate the species of T. amestolkiae. This case emphasizes the importance of suspicion for co-infections and the use of appropriate diagnostic tools.
Article
Mycology
Junichiro Hiruma, Honoka Nojo, Hitoshi Tsuchihashi, Hiromitsu Noguchi, Masataro Hiruma, Kazutoshi Harada, Rui Kano
Summary: In this study, T. interdigitale isolates were obtained from Japanese patients and their ITS types were investigated. The results showed that all 29 isolates belong to ITS type II, while one clinical isolate of T. mentagrophytes was similar to ITS type II*. Additionally, a terbinafine-resistant strain of T. interdigitale also belonged to ITS type II.
Article
Immunology
Gobena Ameni, Alebachew Messele Kebede, Aboma Zewude, Musse Girma Abdulla, Rahel Asfaw, Mesfin Mamo Gobena, Martina Kyalo, Francesca Stomeo, Balako Gumi, Teshale Sori
Summary: This study conducted a phylogenetic analysis of Histoplasma capsulatum isolated from cases of epizootic lymphangitis (EL) in horses in central Ethiopia and evaluated their relationship with isolates from other countries and clades. By analyzing the internal transcribed spacer (ITS) region of rRNA genes, the study identified different clades of H. capsulatum and revealed that the Ethiopian isolates were closely related to isolates from Latin America and Eurasia, but distantly related to isolates from North America.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Plant Sciences
Joanna Sokolowska, Hanna Fuchs, Konrad Celinski
Summary: In this study, we conducted a comprehensive study for the first time to summarize the legitimacy of using the ITS2 region for taxa discrimination among Pinaceae. The obtained results clearly show that its usefulness for this purpose is limited.
Article
Microbiology
Nadia Stoppani, Silvia Colussi, Paolo Pastorino, Marino Prearo, Simona Sciuto, Ilhan Altinok, Rafet Cagri Ozturk, Mustafa Ture, Ana Isabel Vela, Maria del Mar Blanco, Charalampos Kotzamanidis, Konstantina Bitchava, Andigoni Malousi, Lucio Fariano, Donatella Volpatti, Pier Luigi Acutis, Jose Francisco Fernandez-Garayzabal
Summary: The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri for accurate discrimination between these two species. Seven SNPs were identified that separate L. garvieae from L. petauri based on the ITS region sequence. The 16S-23S rRNA ITS region can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.
Article
Biochemistry & Molecular Biology
Elena V. Leshchenko, Dmitrii V. Berdyshev, Ekaterina A. Yurchenko, Alexandr S. Antonov, Gleb V. Borkunov, Natalya N. Kirichuk, Viktoria E. Chausova, Anatoly I. Kalinovskiy, Roman S. Popov, Yuliya V. Khudyakova, Ekaterina A. Chingizova, Artur R. Chingizov, Marina P. Isaeva, Anton N. Yurchenko, Vladimir Kalinin
Summary: Marine-derived fungal strains Penicillium sajarovii and Aspergillus protuberus isolated from sea urchin Scaphechinus mirabilis were found to produce compounds with inhibitory effects on urease activity, bacterial growth, and significant cardioprotective effects in an in vitro model of infectious myocarditis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Fernando Martinez-Hernandez, Fabiola Sanchez-Aguillon, Joel Martinez-Ocana, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Eduardo Lopez-Escamilla, Pablo Maravilla, Guiehdani Villalobos
Summary: This study evaluated the genetic variability of the ITS region and pfor A gene of Trichomonas vaginalis from female patients and explored its implications in the host-parasite relationship. Results showed two main clusters in the ITS haplotypes and suggested that pfor A could be used as a marker for confirming trichomonads genus and species.
Article
Food Science & Technology
Alice Giusti, Enrica Ricci, Laura Gasperetti, Marta Galgani, Luca Polidori, Francesco Verdigi, Roberto Narducci, Andrea Armani
Summary: This study aimed to build an ITS gene dataset to aid the Italian Health Service in mushroom identification. By retrieving and filtering ITS sequences from GenBank and BOLD databases, as well as collecting wild specimens, a dataset of 5977 sequences was successfully established to support regional poisoning cases.
Article
Food Science & Technology
Wassim Habib, Mario Masiello, Hala Chahine-Tsouvalakis, Zahraa Al Moussawi, Carine Saab, Salwa Tohme Tawk, Luca Piemontese, Michele Solfrizzo, Antonio Francesco Logrieco, Antonio Moretti, Antonia Susca
Summary: Lebanon is a major apple producer in the Middle East, but apples stored in cold storage facilities often show signs of decay. The most common fungal disease found in decaying apples is blue mould, mostly caused by Penicillium expansum, which can produce patulin toxin.
Article
Microbiology
Shuqian Hu, Xiang Li, Xin Yin, Runmeng Li, Ruiyang Zhang, Junhao Zang, Yin Liu
Summary: This study developed a species-specific method for identification and classification of Pseudomonas based on ITS sequences combined with next-generation sequencing (NGS).
Article
Microbiology
John. A. Trofymow, Philip-Edouard Shay, Bradley Tomm, Jean A. Berube, Tod Ramsfield
Summary: Fungi play important roles in forest soils and benefit trees through mycorrhizal symbiosis. Changes in fungal communities after severe disturbance can hinder forest regrowth. This study compares soil fungi in undisturbed, harvested, and burned jack pine stands with a certified reclaimed area, and finds that the reclaimed area has greater fungal richness and diversity, including more pathogenic and functionally unknown taxa.
Article
Microbiology
Wael A. Fathy, Natascha Techen, Khaled N. M. Elsayed, Ehab A. Essawy, Eman Tawfik, Khairiah Mubarak Alwutayd, Mohamed S. Abdelhameed, Ola Hammouda, Samir A. Ross
Summary: The absence of proper identification and documentation of microalgae is a common concern in the field of applied phycology, algal physiology, and biochemistry publications. A promising solution lies in employing DNA barcoding techniques and establishing comprehensive databases of reference sequences. This study focuses on the molecular characterization and strain identification of Tetraselmis and Chlorella species using the internal transcribed spacer (ITS) barcode approach.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Peter Halvarsson, Eva Tyden
Summary: Gastrointestinal nematode parasites, especially Strongylus vulgaris, pose a significant threat to horses. Next generation sequencing techniques can accurately diagnose S. vulgaris infections, and the internal transcribed spacer 2 (ITS2) is the best marker for parasitic nematodes. We have provided complete ITS2 sequences for S. vulgaris and S. edentatus, which show high similarity to previously published sequences and amplicon sequence variants (ASV) based on next generation sequencing (NGS). Comparison of Sanger sequencing and NGS methods suggests that NGS is more effective in capturing ITS2 variation. We recommend further NGS sequencing of morphologically identified specimens to cover intraspecific ITS2 variation.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Agronomy
Rachel Mkandawire, Neriman Yilmaz, Emma T. Steenkamp, Marija Kvas, Michael J. Wingfield, Gerda Fourie
Summary: Syzygium cordatum, a commonly encountered tree species in South Africa, was studied to determine the diversity of Fusarium species associated with its inflorescences. Several Fusarium species were identified from both healthy and malformed inflorescences collected from different geographic areas, with FFSC species only found in malformed inflorescences.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Ecology
L. Perini, C. Gostincar, M. Likar, J. C. Frisvad, R. Kostanjsek, M. Nicholes, C. Williamson, A. M. Anesio, P. Zalar, N. Gunde-Cimerman
Summary: Heavily pigmented glacier ice algae reduce the albedo of the Greenland Ice Sheet, contributing to eustatic sea-level rise. In an experimental study, it was found that the fungus P. anthracinoglaciei had a positive relationship with glacier ice algae, while Articulospora sp. did not seem to be involved in pigment conversion. Additionally, the presence of the zoosporic fungus Chytridiomycota increased, suggesting their role as decomposers or parasites of glacier ice algae.
Correction
Multidisciplinary Sciences
Mariska R. Greeff-Laubscher, Karin Jacobs
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Mariska R. Greeff-Laubscher, Karin Jacobs
Summary: This study characterized the Fusarium species isolated from stranded post-hatchling loggerhead sea turtles in South Africa. The isolated strains showed high similarity with Fusarium isolates associated with high egg mortality rates in loggerhead sea turtles, indicating potential harm to the turtles.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Cobus M. M. Visagie, Teun Boekhout, Bart Theelen, Jan Dijksterhuis, Neriman Yilmaz, Keith A. A. Seifert
Summary: A new species of the yeast genus Blastobotrys was discovered in house dust, with similar species detected globally in various substrates. The new species, Blastobotrys davincii, was classified based on morphological, phylogenetic, and physiological characters. The preferred name for the new species is still under debate, but Blastobotrys is argued to be more suitable than Trichomonascus. A nomenclatural review and accepted species list for Blastobotrys/Trichomonascus clade were also provided.
Review
Mycology
Marie L. Overgaard, Trine Aalborg, Emil J. Zeuner, Klaus R. Westphal, Frederik A. Lau, Vibeke S. Nielsen, Kasper B. Carstensen, Emil A. Hundeboll, Tia A. Westermann, Gustav G. Rathsach, Jens L. Sorensen, Jens C. Frisvad, Reinhard Wimmer, Teis E. Sondergaard
Summary: Apiospora and Arthrinium have undergone significant changes in phylogenetic affiliation in the past decade. They have recently been divided into two separate clades, with Apiospora being the majority and several species changing from Arthrinium to Apiospora. The first genome annotation was available in 2020, revealing the high potential for novel secondary metabolite production in these unexplored genera. This review presents the current knowledge of 269 secondary metabolites isolated from these fungal genera, highlighting compounds with known biological or toxic effects. (c) 2022 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
FUNGAL BIOLOGY REVIEWS
(2023)
Article
Mycology
Cobus M. M. Visagie, Neriman Yilmaz
Summary: In this study, the diversity of Penicillium in soil collected from Woodville Big Tree Forest Trail near the coastal town of Wilderness in South Africa was investigated. Through DNA sequencing, 18 species were identified, including six undescribed species. Morphological characteristics and phylogenetic comparisons were used to distinguish these new species.
Review
Biochemistry & Molecular Biology
Xinhui Wang, Scott A. Jarmusch, Jens C. Frisvad, Thomas O. Larsen
Summary: This review covers all known biosynthetic compound families and their structural diversity from black aspergilli, with a focus on metabolites that have been firmly linked to their corresponding BGCs.
NATURAL PRODUCT REPORTS
(2023)
Article
Food Science & Technology
Oceance Savary, Emmanuel Coton, Marie-Bernadette Maillard, Frederic Gaucheron, Christophe Le Meur, Jens Frisvad, Anne Thierry, Jean-Luc Jany, Monika Coton
Summary: This study aimed to evaluate the functional impact of Bisifusarium domesticum and other cheese-associated fungi on cheese-making. Their lipolytic and proteolytic activities, as well as their potential to produce volatile and non-volatile secondary metabolites, were assessed. Four novel cheese-associated species, including Bisifusarium allantoides, Bisifusarium penicilloides, Longinectria lagenoides, and Long-inectria verticilliformis, were described, and these species showed potential in lipid production.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Mycology
B. van der Merwe, P. Herrmann, Karin Jacobs
Summary: A new species of Hericium, named Hericium ophelieae sp. nov., was recently discovered in the Afrotemperate forests of Southern Africa. This novel species has dentate features common to other Hericium species but exhibits distinct substrate choice, gloeocystidia, and basidiospore sizes. Molecular sequencing confirmed its uniqueness and revealed that it is the first endemic species of the Hericium genus in Southern Africa. The study emphasizes the need for further research on fungal diversity in Afrotemperate environments.
MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY
(2023)
Article
Mycology
Felipe Balocchi, Irene Barnes, Michael J. Wingfield, Rodrigo Ahumada, Cobus M. Visagie
Summary: In this study, a previously unknown fungus found on ancient Araucaria trees in Chile and Argentina was identified as two new species, and was classified as Resinogalea genus within the Cryptocaliciomycetidae subclass based on morphological and molecular analyses.
Article
Mycology
Celine Petersen, Trine Sorensen, Mikkel R. Nielsen, Teis E. Sondergaard, Jens L. Sorensen, David A. Fitzpatrick, Jens C. Frisvad, Kare L. Nielsen
Summary: In this study, researchers sequenced 93 Penicillium isolates and established a species phylogeny and pangenome. They found that approximately half of the genes are shared among 98 or more isolates. They also discovered 15 lateral gene transfer events that have played a role in the evolution and adaptation of these fungi. This comprehensive characterization of the Penicillium genus surpasses single-reference genomes and captures the genetic variation.
Article
Electrochemistry
Charlotte Overgaard Wilhelmsen, Sebastian Birkedal Kristensen, Oliver Nolte, Ivan A. Volodin, Johan Vormsborg Christiansen, Thomas Isbrandt, Trine Sorensen, Celine Petersen, Teis Esben Sondergaard, Kare Lehmann Nielsen, Thomas Ostenfeld Larsen, Jens Christian Frisvad, Martin D. Hager, Ulrich S. Schubert, Jens Muff, Jens Laurids Sorensen
Summary: Aqueous organic redox flow batteries (AORFBs) are gaining interest as a potential solution for sustainable energy storage. In this study, a new electrolyte based on the fungal compound phoenicin was proposed and produced through biosynthesis, offering scalability. It was demonstrated that biologically produced phoenicin as a negative electrolyte showed promising performance in an RFB, and the stability of the active material was investigated.
BATTERIES & SUPERCAPS
(2022)
