Article
Chemistry, Analytical
Vincen Wu, Jocelyn Tillner, Emrys Jones, James S. McKenzie, Dipa Gurung, Anna Mroz, Liam Poynter, Daniel Simon, Cristina Grau, Xavier Altafaj, Marc-Emmanuel Dumas, Ian Gilmore, Josephine Bunch, Zoltan Takats
Summary: This study examines the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue, comparing its performance to the traditional desorption electrospray ionization (DESI). The flow focusing mechanism in DESI offers advantages such as a rotationally symmetric electrospray jet, higher intensity, controllable parameters, and better portability. The optimized parameters for DEFFI result in similar images to DESI but with better sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Juha Kangasluoma, Jyri Mikkila, Verner Hemmila, Oskari Kausiala, Jani Hakala, Evgenia Iakovleva, Paxton Juuti, Mikko Sipila, Heikki Junninen, H. J. Jost, Aleksei Shcherbinin
Summary: Illegal explosives pose a threat to public safety, aviation, transportation, and critical infrastructure. Researchers have developed a trace explosives detection instrument with high sensitivity and efficient workflows. The instrument utilizes thermal desorption of filters for the analysis of liquid and solid phase samples, followed by selective atmospheric pressure chemical ionization and detection with a mass spectrometer. The experiments demonstrated that the system has low detection limits and can effectively detect various common explosives.
Article
Chemistry, Analytical
Alexander Pevzner, Gil Feldheim, Amalia Zaltsman, Shmuel Elisha, Vered Heleg-Shabtai, Izhar Ron
Summary: The need for sampling hazardous compounds in the form of solids and liquids is increasing in the fields of homeland security and forensics. The approach presented in this work utilizes sonic spray and hand-held ion mobility spectrometry for rapid, continuous vapor generation and analysis of liquid samples, providing competitive performance to common thermal desorption techniques. The technique is simple, cost-effective, and suitable for field use.
Review
Spectroscopy
Maria del Mar Boronat Ena, David A. Cowan, Vincenzo Abbate
Summary: In the past decade, numerous drugs with similar effects to controlled psychoactive drugs have been synthesized, known as new psychoactive substances (NPS). These substances are used for recreational purposes, but their detection and legal control pose significant challenges due to their novelty and structural variability. As a result, there is an increasing demand for rapid and easy-to-use analytical techniques for their detection and identification. Ambient ionization mass spectrometry has gained interest in recent years due to its speed, simplicity, and low cost, making it suitable for analyzing NPS in the forensic and clinical toxicology fields.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Chemistry, Analytical
Liang Chen, Alireza Ghiasvand, Estrella Sanz Rodriguez, Peter C. Innis, Brett Paull
Summary: Ambient ionization mass spectrometry (AIMS) has developed rapidly in recent years with the introduction of various new ionization techniques, while the application of nanomaterials has also received significant attention, effectively improving selectivity and ionization efficiency.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Hilary M. Brown, Patrick W. Fedick
Summary: 3D-PCSI-MS is a rapid method for detecting trace PFAS in solid media through ambient ionization mass spectrometry, with advantages of low cost, scalability, and high-throughput screening of bulk samples without sample preparation.
Review
Chemistry, Analytical
Vera Plekhova, Kimberly De Windt, Margot De Spiegeleer, Marilyn De Graeve, Lynn Vanhaecke
Summary: Biofluid metabolomics is a valuable tool for discovering biomarkers related to disease, genetics, and exposure. Direct infusion and ambient ionization mass spectrometry offer attractive alternatives for rapid analysis with minimal sample handling.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Geochemistry & Geophysics
Yankun Di, Evgenii Krestianinov, Sonja Zink, Yuri Amelin
Summary: High-precision measurement of Sr-84/Sr-86 variations using TIMS is crucial for understanding planetary volatile depletion history and isotopic reservoirs in the early Solar System. Discrepancies between static and multidynamic TIMS measurements indicate biases, with fractionation drift significantly affecting the Sr-84/Sr-86 ratio. Corrections for these effects reveal an accurate average mu Sr-84 value for terrestrial samples and highlight differences between standard and natural samples.
Article
Biochemistry & Molecular Biology
Hung Su, Zong-Han Jiang, Shu-Fen Chiou, Jentaie Shiea, Deng-Chyang Wu, Sung-Pin Tseng, Shu-Huei Jain, Chung-Yu Chang, Po-Liang Lu
Summary: An analytical platform combining probe sampling, thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS), and multivariate statistical analysis was developed to rapidly differentiate bacterial species based on the differences in their lipid profiles. The results were validated by protein fingerprinting using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF). The developed platform showed promising potential as a useful diagnostic tool for rapidly distinguishing bacterial species.
Article
Chemistry, Analytical
Xiaoping Zhang, Xiang Ren, Yuan Zhong, Konstantin Chingin, Huanwen Chen
Summary: The study detected ketones in exhaled breath using mass spectrometry and developed a fast, sensitive, noninvasive method for ketone analysis. Results showed higher levels of acetone in the breath of smokers compared to non-smokers.
Review
Spectroscopy
Timothy Vazquez, Sinduri Vuppala, Ifeoluwa Ayodeji, Linxia Song, Nathan Grimes, Theresa Evans-Nguyen
Summary: Mass spectrometry (MS) plays a crucial role in exploring the chemical composition of our solar system, with applications in various space missions to analyze inorganic compositions and molecular analyses. As technology advances, MS instruments continue to evolve to provide more sophisticated and powerful tools for future space exploration endeavors.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Multidisciplinary Sciences
Margot De Spiegeleer, Vera Plekhova, Jozefien Geltmeyer, Ella Schoolaert, Beata Pomian, Varoon Singh, Kathleen Wijnant, Kimberly De Windt, Volter Paukku, Alexander De Loof, Inge Gies, Nathalie Michels, Stefaan De Henauw, Marilyn De Graeve, Karen De Clerck, Lynn Vanhaecke
Summary: In recent years, ambient ionization mass spectrometry (AIMS) has allowed for direct analysis of biofluid metabolome. However, AIMS procedures are hindered by analytical and practical limitations, which affect metabolome coverage. This study aimed to develop biofluid-specific metabolome sampling membranes (MetaSAMPs) to improve AIMS. Customized MetaSAMPs made of hydrophilic and lipophilic polymers demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis. This new approach was successfully validated and has potential applications in metabolic health stratification.
Article
Biochemical Research Methods
Christopher C. Goudarzi, Ashley G. Woolfork, Arjun Sengupta, Aalim M. Weljie
Summary: Increased access to cheap and rapid mass spectrometry testing of biofluids is desired for the analysis of disorders and diseases related to metabolite or lipid level changes. This study establishes a customized high-throughput workflow for biological sample analysis using DESI-MS, which utilizes low-cost, open-source, and readily accessible materials. The workflow includes surface customization, high-throughput sample spotting, and an open-source interface for data analysis. The developed system allows for cost-effective custom surface design workflows and high-throughput analyses for a wide range of researchers.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Luan Felipe Campos Oliveira, David Ulisses Tega, Marcos Nogueira Eberlin, Alessandra Sussulini
Summary: V-EASI is a soft ambient ionization source suitable for samples in solution, without the need for voltage or heat, with low sample preparation requirements. The study validated the quantification method for bixin in annatto using the LC-V-EASI-MS system, showing good performance.
Review
Spectroscopy
Hanlu Yue, Feiyao He, Zhongjun Zhao, Yixiang Duan
Summary: Ambient mass spectrometry (AMS) is a group of advanced analytical techniques that enable direct sampling and ionization of analytes in their native environment, offering advantages such as rapid and real-time analysis. Plasma-based AMS, as a significant category of AMS, has been widely applied in various fields and has undergone continuous development and innovation.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Biochemical Research Methods
J. Tyler Davidson, Zachary J. Sasiene, Glen P. Jackson
DRUG TESTING AND ANALYSIS
(2020)
Article
Chemistry, Analytical
Praneeth M. Mendis, Zachary J. Sasiene, David Ropartz, Helene Rogniaux, Glen P. Jackson
Summary: This study successfully coupled charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins. Compared to UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
J. Tyler Davidson, Zachary J. Sasiene, Glen P. Jackson
Summary: In-source CID and beam-type CID are two methods for mass spectrometry analysis, with the former not requiring an isolation step before collisional activation, resulting in a product ion spectrum composed of fragment ions from any analyte present during the activation event, while the latter involves isolation before collision. The study results show that large fragmentor potentials for in-source CID favor the generation of more abundant lower mass product ions compared to beam-type CID.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Physics, Atomic, Molecular & Chemical
Zachary J. Sasiene, Praneeth M. Mendis, Glen P. Jackson
Summary: The study explores the potential of using cheaper and more readily available reagent gases to replace helium in CTD-MS. The results show that different reagent gases have minimal impact on the activation of biological ions in CTD, suggesting that the ionization energy of the reagent gas has negligible effect on the fragmentation efficiency.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Praneeth M. Mendis, Zachary J. Sasiene, David Ropartz, Helene Rogniaux, Glen P. Jackson
Summary: Ultra-high-performance liquid chromatography with charge transfer dissociation mass spectrometry was used to analyze a mixture of complex sulfated oligosaccharides, showing more abundant ions and less neutral losses compared to collision-induced dissociation. Optimization of conditions led to more confident structural detail, highlighting the compatibility of He-CTD with UHPLC and the enhanced structural information provided.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Zachary J. Sasiene, David Ropartz, Helene Rogniaux, Glen P. Jackson
Summary: The impact of metal ions on the mass and charge state of product ions was observed, while the influence on the types of cross-ring cleavages was found to be negligible. Helium charge transfer dissociation (He-CTD) generated structurally informative glycosidic and cross-ring cleavages for branched glycan adducts, while low-energy collision-induced dissociation (LE-CID) mainly produced glycosidic cleavages, consecutive fragments, and neutral losses that complicated spectral interpretation.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Halle M. Edwards, Hoi-Ting Wu, Ryan R. Julian, Glen P. Jackson
Summary: Understanding a protein's function relies on its detailed structure, with even small changes in the primary structure potentially disrupting function. Long-lived proteins are especially vulnerable to alterations in primary sequence, leading to diseases. Charge transfer dissociation (CTD) can differentiate between isomeric and epimeric forms of Asp in peptides, offering a robust and statistically significant method for analysis.
Article
Physics, Atomic, Molecular & Chemical
Zachary J. Sasiene, Praneeth M. Mendis, David Ropartz, Helene Rogniaux, Glen P. Jackson
Summary: This study focused on the application of charge transfer dissociation (CTD) to mannuronic acid oligomer with different Na/H exchange levels. CTD provided unique and unambiguous fragments, but precursors with more Na/H exchanges resulted in increased ambiguity in spectral assignments. The study suggests that precursors with fewer Na/H exchanges will provide more confident peak assignments and structural characterization until CTD is coupled to high-resolution mass spectrometers.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Halle M. Edwards, Zachary J. Sasiene, Praneeth M. Mendis, Glen P. Jackson
Summary: Research in natural products is important in addressing issues with drug resistance, drug delivery, and emerging diseases. However, the structural complexity of natural products presents challenges for their analysis and synthesis. CTD-MS is a useful tool in characterizing natural and synthetic macrocycles, providing rich and informative spectra that help identify modification sites and resolve structural analogues.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Halle M. Edwards, Hoi-Ting Wu, Ryan R. Julian, Glen P. Jackson
Summary: The study developed a new mass spectrometry method using a modified three-dimensional ion trap for charge transfer dissociation (CTD) analysis. This method can accurately identify Leu/Ile residues with high sensitivity, and it shows comparable performance with other high-energy activation techniques.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Samantha A. Mehnert, J. Tyler Davidson, Alexandra Adeoye, Brandon D. Lowe, Emily A. Ruiz, Jacob R. King, Glen P. Jackson
Summary: This study demonstrates the use of binary classifiers and GLM to enable specific and selective identifications of mass spectra from different laboratories, with lower error rates compared to traditional approaches.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Review
Biochemical Research Methods
Glen P. Jackson, Mark A. Barkett
Summary: Mass spectrometry has significantly contributed to the criminal justice system by offering precise analytical methods and reliable scientific evidence. It has been applied to a wide range of samples and analytes, from trace metal impurities to drugs, explosives, polymers, and ignitable liquids. This review discusses the historical developments, connects them to real cases, and explores the evolving consensus standards. Although mass spectrometry has a strong foundation and a successful track record, it is just one piece of the legal puzzle, as evidenced by some appellate decisions that did not solely rely on the mass spectrometric data.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Glen P. P. Jackson, Samantha A. A. Mehnert, J. Tyler Davidson, Brandon D. D. Lowe, Emily A. A. Ruiz, Jacob R. R. King
Summary: This study aims to accurately identify an organic substance from its mass spectrum without analyzing a contemporaneous spectrum of the suspected substance. The first part of the report describes how a theoretical model predicts linear correlations between branching ratios in mass spectra under changing analysis conditions. The model was tested using a dataset of cocaine spectra, achieving accurate predictions even with significant variations in measured abundances. The second part of the report demonstrates how the model can be applied for reliable identification of cocaine and its diastereomers from other known negative spectra.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Praneeth M. Mendis, Glen P. Jackson
Summary: The combination of helium charge transfer dissociation mass spectrometry with ultrahigh performance liquid chromatography provides a powerful tool for the analysis of complex mixtures of human milk oligosaccharides. This method allows for the identification of monosaccharide sequence, branching patterns, sialylation/fucosylation arrangements, and differentiation of isomeric oligosaccharides. The results demonstrate the compatibility of helium charge transfer dissociation with ultrahigh performance liquid chromatography and its ability to provide informative fragments for differentiation of alpha-2,3- versus alpha-2,6-linked sialic acid residues and differentiation of structural isomers.
Article
Chemistry, Analytical
Mayara P. Matos, Marc E. Engel, John B. Mangrum, Glen P. Jackson
Summary: A new combined method involving multivariate statistical analysis and isotope analysis on samples of Eastern oysters has shown promise in predicting geographic origin and achieving a high success rate in discriminating between different harvesting areas.
ANALYTICAL METHODS
(2021)