Article
Chemistry, Physical
Judit E. Puskas, Gayatri Shrikhande, Kristof Molnar
Summary: This study investigated the synthesis of fluorescein-diacrylate (FL-DA) and four-functional fluoresceins (FL) through the Michael addition of secondary amines to FL-DA. FL-DA was successfully synthesized at a scale of 100 g. Diallyl amine (DAA) rapidly reacted with FL-DA, while diethanolamine (DEA) had a longer reaction time and was unstable. The addition reactions of aminodiols with longer carbon chains and diethyl-amino-diacetate (DIDA) were slower but could be accelerated using Candida antarctica lipase B (CALB) as a biocatalyst. The structure of tetrafunctional fluoresceins was confirmed by 13C and 1H NMR, and high resolution Mass Spectrometry (HRMS) demonstrated the formation of highly pure tetraallyl- and tetraethylacetate-fluoresceins. These multifunctional fluoresceins show promise for the synthesis of imaging agents for various applications.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biotechnology & Applied Microbiology
Liwen Shu, Xin Zheng, Shuhua Qi, Shuizhi Lin, Yinghua Lu, Chuanyi Yao, Xueping Ling
Summary: This study investigated the enzymatic transesterification of algal oil to prepare DHA-PC using immobilized Candida antarctica lipase B. The optimized reaction conditions resulted in a high PC content of 74.8% within 72 hours. The immobilized enzyme exhibited good operational stability and can be used as a biocatalyst for DHA-PL synthesis.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Natasha K. Maddigan, Oliver M. Linder-Patton, Paolo Falcaro, Christopher J. Sumby, Stephen G. Bell, Christian J. Doonan
Summary: The biomimetic mineralization of CALB@ZIF-8 biocomposites was studied by altering Zn2+ and 2-methylimidazole concentrations, revealing that specific synthetic conditions can maintain the catalytic activity of CALB, with phosphate buffer aiding in the recovery of activity for larger particles. Additionally, transesterification reactions in 100% hexane showed enhanced CALB activity compared to free enzyme. The study highlights the significance of synthetic protocols and operating parameters in developing enzyme@MOF biocomposites with improved activity in challenging conditions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Kholoud El-Kady, Mai Raslan, Ayman H. Zaki
Summary: In this study, the immobilization of lipase from Aspergillus niger on TiO2 nanoparticles with different morphologies was investigated. The lipase immobilized on TiO2 microspheres showed the highest activity, while the hydrolytic activity was found to be the highest at a microsphere/lipase ratio of 1:0.25. Additionally, the immobilization yield activities were higher for lower microsphere/lipase ratios.
Article
Chemistry, Physical
Koen Adriaensen, Jannick Vercammen, Cedric Van Goethem, Kumar Varoon Agrawal, Dirk De Vos
Summary: In this study, a chemoenzymatic dynamic kinetic resolution (DKR) method for beta-amino esters was established through the evaluation of metal-based heterogeneous catalysts for racemization and enzyme catalysts for kinetic resolution. By selecting two promising racemization catalysts, the researchers established a relationship between catalyst characteristics and reaction selectivities, and achieved efficient DKR with excellent enantioselectivity.
CATALYSIS SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zeping Lu, Mei Chen, Tongtong Jin, Binbin Nian, Yi Hu
Summary: Ionic liquids (ILs) are used as chemical modifiers to improve the efficiency, activity, and stability of enzymes. This study investigated the impact of ILs with different chain lengths on the enzymatic properties of Candida antarctica lipase B (CALB) and found that ILs significantly influenced CALB's absorption orientation. Molecular dynamics simulations showed that ILs with suitable chain lengths formed strong interactions with CNTs and ILs, stabilizing CALB's open state and improving its activity and stability. This study provides insights into the design of green modifiers for better immobilized enzymes and lays the groundwork for future research on IL modifiers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Jose Jesus Guzman-Mendoza, David Chavez-Flores, Silvia Lorena Montes-Fonseca, Carmen Gonzalez-Horta, Erasmo Orrantia-Borunda, Blanca Sanchez-Ramirez
Summary: The study utilized Candida antarctica lipase to functionalize insulin onto the surface of carbon nanotubes, achieving a higher efficiency and greener process compared to previous methods. This enzymatic esterification method can be applied to enhance cellular-specific release of proteins.
Article
Green & Sustainable Science & Technology
Hyunjun Ko, Mi-Jin Kim, Hyun-Jin Kim, Jin Kang, Ho-Yeon Lee, Jin Hyuk Lee, Jung-Hoon Bae, Bong Hyun Sung, Jung-Hoon Sohn
Summary: Food waste oil is a suitable raw material for biodiesel production, but water inhibits the reaction. By developing a mutant lipase CALB1422, ester synthesis can be catalyzed in the presence of water, resulting in higher conversion rates and yields of biodiesel from waste oil.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Shanxiu Ming, Shuyi Li, Zhe Chen, Xujun Chen, Feifei Wang, Shaonan Deng, Krystian Marszalek, Zhenzhou Zhu, Wenxiang Zhang, Francisco J. Barba
Summary: Traditional enzymatic esterification methods have limitations in improving the bioavailability of flavonoids, while the novel BLIMs technology shows great potential in enhancing compound utilization. Among the three BLIMs, GA/CAL-B@PDA/PES performs the best with high enzyme activity and loading, as well as stable performance under various environmental conditions.
Article
Multidisciplinary Sciences
Amir Hossein Fattahi, Mohammad G. Dekamin, James H. Clark
Summary: Ball-milled seashells in the form of nano-biocomposite catalyst with fixed CO2 in its aragonite microcrystalline structure were used to optimize the synthesis of isoamyl acetate. The seashells nano-biocomposite proved to be an excellent catalyst for the green and environmentally-friendly synthesis of isoamyl acetate under solvent-free conditions. The optimized conditions resulted in a high yield of 91%, with the advantages of using an inexpensive and easily prepared nano-biocomposite material without hazardous reagents or corrosive acids.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Physical
Honghai Wang, Wenda Yue, Shuling Zhang, Yu Zhang, Chunli Li, Weiyi Su
Summary: The study demonstrated a simple and highly effective strategy for stabilizing enzymes embedded inside silica xerogels by a polydopamine (PDA) coating. The modified silica xerogels showed improved loading ability, retention of enzyme activities, as well as mechanical properties, thermal stability and reusability. These advantages make the new hybrid material a promising support for immobilized enzymes.
Article
Biochemistry & Molecular Biology
Liliana Giraldo, Fernando Gomez-Granados, Juan Carlos Moreno-Pirajan
Summary: This research immobilizes CALB on MOF-199 and ZIF-8 and uses them in the transesterification reaction for biodiesel production. The highest adsorption capacity of 26.9 mg•g-1 Lipase is achieved using ZIF-8 at 45°C and an initial protein concentration of 1.20 mg•mL-1. CALB is physically adsorbed on ZIF-8 and chemically adsorbed on MOF-199. Kinetic studies show that the adsorption kinetics follow the Elovich model for both biocatalysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Nipon Sarmah, Vazida Mehtab, Lakshmi Sai Pratyusha Bugata, James Tardio, Suresh Bhargava, Rajarathinam Parthasarathy, Sumana Chenna
Summary: In this study, a hybrid machine learning aided experimental approach was proposed to evaluate the growth kinetics of Candida antarctica for lipase production. The Gradient boosting regression (GBR) model showed superior performance in predicting the growth curves. The application of this hybrid approach significantly reduced the experimental effort, time, and resources, while matching well with the results of the conventional experimental approach. Additionally, the activity and enzyme kinetics of the lipase produced were investigated and the robustness of the kinetic models were ensured through statistical analysis.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Organic
Seonghyeon Yi, Seongsoon Park
Summary: In this study, the researchers enhanced the enantioselectivity of CAL-B towards (+/-)-but-3-yn-2-ol and (+/-)-butan-2-ol by substituting the local sequence of CAL-B with that of PBL. The sequence-substituted mutant of CAL-B exhibited significantly higher enantioselectivity towards (+/-)-but-3-yn-2-ol (E > 200) and (+/-)-butan-2-ol (E > 25).
TETRAHEDRON LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Helena D. Tjornelund, Jesper Vind, Jesper Brask, John M. Woodley, Gunther H. J. Peters
Summary: This study investigates the molecular mechanism of the specific activity of Candida antarctica lipase B (CALB) in low-water environments using molecular dynamics simulations. Based on experiments and simulations, four criteria influencing the performance of the enzyme in organic media were proposed and it was shown that specific mutants can resist changes in water activity.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
