Journal
MOLECULES
Volume 20, Issue 5, Pages 7574-7585Publisher
MDPI
DOI: 10.3390/molecules20057574
Keywords
nitric oxide; tanshinone; Salvia miltiorrhiza; polyethylene glycol; abscisic acid
Funding
- National Natural Science Foundation of China [81373908, 81403033]
- Public Project of Technological Application in Zhejiang Province [2014C32108]
- Zhejiang Provincial Natural Science Foundation of China [LQ13C020003]
- Shaanxi science and technology plan projects [2012K19-02-02]
- China Postdoctoral Science Foundation [2014M551771]
- Science Foundation of Zhejiang Sci-Tech University [1204806-Y]
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Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantly stimulated by sodium nitroprusside (SNP, a NO donor) at 100 M. Effects of SNP were just partially arrested by the mevalonate (MVA) pathway inhibitor (mevinolin), but were completely inhibited by the 2-C-methyl-d-erythritol-4-phosphate pathway (MEP) inhibitor (fosmidomycin). The increase of tanshinone accumulation and the up-regulation of HMGR and DXR expression by PEG and ABA treatments were partially inhibited by an inhibitor of NO biosynthesis (N-nitro-L-arginine methyl ester (L-NAME)) and a NO scavenger (2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO)). Simultaneously, NO generation in the hairy roots was triggered by PEG and ABA, and the effects were also arrested by c-PTIO and L-NAME. These results indicated that NO signaling probably plays a central role in water stress-induced tanshinone production in S. miltiorrhiza hairy roots. SNP mainly stimulated the MEP pathway to increase tanshinone accumulation.
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