Lectin Engineering, a Molecular Evolutionary Approach to Expanding the Lectin Utilities

Engineering of a 3′-sulpho-Galβ1-4GlcNAc-specific probe by a single amino acid substitution of a fungal galectin

(2015) Dan Hu et al.   JOURNAL OF BIOCHEMISTRY

rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells

(2013) Yasuko Onuma et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Tailoring GalNAcα1-3Galβ-specific lectins from a multi-specific fungal galectin: dramatic change of carbohydrate specificity by a single amino-acid substitution

(2013) Dan Hu et al.   BIOCHEMICAL JOURNAL

Conformational change of a unique sequence in a fungal galectin fromAgrocybe cylindraceacontrols glycan ligand-binding specificity

(2013) Naoyuki Kuwabara et al.   FEBS LETTERS

Optimization of the inter-domain structure of galectin-9 for recombinant production

(2013) Aiko Itoh et al.   GLYCOBIOLOGY

Single-Site Mutational Engineering and Following MonoPEGylation of the Human Lectin Galectin-2: Effects on Ligand Binding, Functional Aspects, and Clearance from Serum

(2013) Jürgen Kopitz et al.   MOLECULAR PHARMACEUTICS

Deciphering the Glycan Preference of Bacterial Lectins by Glycan Array and Molecular Docking with Validation by Microcalorimetry and Crystallography

(2013) Jeremie Topin et al.   PLoS One

Easy preparation of a large-size random gene mutagenesis library in Escherichia coli

(2012) Chun You et al.   ANALYTICAL BIOCHEMISTRY

Directed Evolution of Lectins with Sugar-binding Specificity for 6-Sulfo-galactose

(2012) Dan Hu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural and Quantitative Evidence for Dynamic Glycome Shift on Production of Induced Pluripotent Stem Cells

(2012) Kayo Hasehira et al.   MOLECULAR & CELLULAR PROTEOMICS

Modification of the sugar specificity of a plant lectin: structural studies on a point mutant ofErythrina corallodendronlectin

(2011) Subbiah Thamotharan et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Lectin-based structural glycomics: A practical approach to complex glycans

(2011) Jun Hirabayashi et al.   ELECTROPHORESIS

Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis

(2011) David P Nannemann et al.   Future Medicinal Chemistry

Engineering of the glycan-binding specificity of Agrocybe cylindracea galectin towards α(2,3)-linked sialic acid by saturation mutagenesis

(2011) Koji Imamura et al.   JOURNAL OF BIOCHEMISTRY

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

(2011) Hiroaki Tateno et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Recombinant chimeric lectins consisting of mannose-binding lectin and L-ficolin are potent inhibitors of influenza A virus compared with mannose-binding lectin

(2010) Wei-Chuan Chang et al.   BIOCHEMICAL PHARMACOLOGY

Multilectin Assay for Detecting Fibrosis-Specific Glyco-Alteration by Means of Lectin Microarray

(2010) A. Kuno et al.   CLINICAL CHEMISTRY

Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin

(2010) Johan Olausson et al.   GLYCOBIOLOGY

A Novel L-ficolin/Mannose-binding Lectin Chimeric Molecule with Enhanced Activity against Ebola Virus

(2010) Ian C. Michelow et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Functional β-propeller lectins by tandem duplications of repetitive units

(2010) Itamar Yadid et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Glycomic Analysis: An Array of Technologies

(2009) Lakshmi Krishnamoorthy et al.   ACS Chemical Biology

Engineering a versatile tandem repeat-type α2-6sialic acid-binding lectin

(2009) Rikio Yabe et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris

(2009) Hikaru Hemmi et al.   FEBS Journal

Sugar-binding activity of the MRH domain in the ER  -glucosidase II   subunit is important for efficient glucose trimming

(2009) D. Hu et al.   GLYCOBIOLOGY

The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation

(2009) Kaoru Mikami et al.   GLYCOBIOLOGY

A Library of MutatedMaackia amurensisHemagglutinin Distinguishes Putative Glycoforms of Immunoglobulin A1 from IgA Nephropathy Patients†

(2009) Keisuke Maenuma et al.   JOURNAL OF PROTEOME RESEARCH

GlycoBase and autoGU: tools for HPLC-based glycan analysis

(2008) M. P. Campbell et al.   BIOINFORMATICS

Single-Site Cys-Substituting Mutation of Human Lectin Galectin-2: Modulating Solubility in Recombinant Production, Reducing Long-Term Aggregation, and Enabling Site-Specific MonoPEGylation

(2008) Hui Wang et al.   BIOMACROMOLECULES

 2-6 Linked Sialic Acids on N-Glycans Modulate Carcinoma Differentiation In vivo

(2008) M. Hedlund et al.   CANCER RESEARCH

Mass Spectrometry and the Emerging Field of Glycomics

(2008) Joseph Zaia   CHEMISTRY & BIOLOGY

Glycoconjugate microarray based on an evanescent-field fluorescence-assisted detection principle for investigation of glycan-binding proteins

(2008) H. Tateno et al.   GLYCOBIOLOGY

A simple strategy for the creation of a recombinant lectin microarray

(2008) Ku-Lung Hsu et al.   Molecular BioSystems

Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)

(2008) Amy J. Baldwin et al.   NUCLEIC ACIDS RESEARCH

Use of a library of mutated Maackia amurensis hemagglutinin for profiling the cell lineage and differentiation

(2008) Keisuke Maenuma et al.   PROTEOMICS

Evolution of the β-propeller fold

(2007) Indronil Chaudhuri et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS