Assessing the subsequent effect of nitrogen released from tobacco-waste on maize crop using a 15N isotope technique

The investigation of the residual effect of nitrogen (N) released from tobacco-waste (TW) using isotope techniques will provide valuable data for sustainable organic farming. For this aim, a pot experiment was conducted using the N-15 isotope technique. The experiment was based on a completely randomised design with four replications and was conducted on a calcareous ustochrepts soil. TW at levels of 0, 10, 20, 30 and 40tha(-1) and N fertiliser as (NH4)(2)SO4 at levels of 0, 20, 40, 60 and 80kgNha(-1) were used for the Bezostaja-1 wheat variety. Concerning mineral N fertilisation with 20 and 80kgNha(-1), additional treatments with N-15-labelled (NH4)(2)SO2 (10 at.% exc.) have been applied. Following harvesting wheat plants, the Pioneer 3377 maize variety was used to see the residual effect of TW. After harvesting, dry matter yields were recorded and total N concentrations were determined. N-15 determinations and calculations were also made for N-15 treatments separately. TW had a significant residual effect on the growth of corn plant under the pot condition. Increasing rates of TW significantly increased the dry matter yield of corn plant following wheat from 3.31tha(-1) (at control) to 7.89tha(-1) (at the TW treatment of 40tha(-1)). The N-15 values derived from the N-15 fertiliser decreased with increasing TW application. The average values of N derived from N fertiliser (Ndff) varied from 2.14 to 3.09% at the rates of 20 and 80kgNha(-1), respectively. However, N derived from TW (Ndftw) significantly increased from 16.93 to 24.59% (at 20kgNha(-1)), and it also increased from 23.06 to 28.15% (at 80kgNha(-1)) with increasing TW applications from 20 to 40tha(-1), respectively.