Article
Cell Biology
September Numata, Jeffrey P. McDermott, Gustavo Blanco
Summary: The NKA alpha 4 isoform is specifically expressed in male germ cells and plays a crucial role in sperm motility and energetics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Peter W. Chomczynski, Kianna M. Vires, Michal Rymaszewski, Judith A. Heiny
Summary: The cardiotonic steroid binding site on Na,K-ATPase plays a vital role in cell processes and is a therapeutic target for human disease. Mouse models with altered affinity for cardiotonic steroids have been developed and a rapid genotyping method has been developed to advance their use for studying the physiological roles and drug actions.
Article
Biochemistry & Molecular Biology
Bin Leng, Lin Deng, Jianxin Tan, Wei-Thye Lee, Cheng-Rui Cao, Zi-Ping Wang, De-Jian Huang, Xiaowei Nie, Jin-Song Bian
Summary: Doxorubicin (DOX) is a potent chemotherapeutic drug but its use is limited due to cardiotoxic side effects. This study investigates the role of Na+/K+ ATPase (NKA) in DOX-induced cardiotoxicity and ferroptosis, and proposes NKA as a potential therapeutic target for DIC.It was found that a decrease in NKA activity aggravated DOX-induced cardiac dysfunction and ferroptosis, while antibodies against the DR-region of NKAa-subunit (DR-Ab) attenuated these effects.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Neurosciences
Angelica S. Reis, Carolina C. Martins, Ketlyn P. da Motta, Jaini J. Paltian, Gabriel P. Costa, Diego Alves, Cristiane Luchese, Ethel Antunes Wilhelm
Summary: This study evaluates the impact of aging on oxaliplatin-induced acute peripheral neuropathy and explores the potential of 7-chloro-4-(phenylselanyl) quinoline as a new therapeutic strategy. The results show that aging exacerbates the pain caused by oxaliplatin through increased oxidative damage and inhibition of Na+, K+-ATPase and Mg2+-ATPase. However, 4-PSQ can reverse the hypersensitivity induced by oxaliplatin and aging by reducing ROS and NOx levels and modulating GPx and SOD activities.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jing Liu, Gillian M. Schiralli-Lester, Rosemary Norman, David A. Dean
Summary: Acute Lung Injury/Acute Respiratory Distress Syndrome (ALI/ARDS) is characterized by damaged alveoli and edema accumulation, leading to respiratory failure. Gene delivery of Na+, K+-ATPase beta 1 subunit improves alveolar fluid clearance and barrier function, while MRCK alpha gene delivery enhances epithelial and endothelial barrier integrity for ARDS treatment. This study investigates the therapeutic potential of beta 2 and beta 3 subunits in LPS-induced ALI, and found that they do not show the same benefits in lung injury treatment as the beta 1 subunit, suggesting that improving barrier function may be more beneficial than fluid clearance alone in ALI/ARDS treatment.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Marta Marchini, Mitchell R. Ashkin, Melina Bellini, Margaret Man-Ger Sun, Matthew Lloyd Workentine, Hamza Malik Okuyan, Roman Krawetz, Frank Beier, Campbell Rolian
Summary: The genetic and developmental mechanisms of limb formation are well understood, but how chondrocyte physiology modulates these mechanisms to produce differences in limb bone length is unclear. Through RNA sequencing and cell culture experiments, it was found that NKA plays a key role in chondrocyte differentiation and regulation of bone length, with implications for limb length diversity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jeff P. McDermott, Gladis Sanchez, Amrita Mitra, September Numata, Lijun Catherine Liu, Gustavo Blanco
Summary: In mammalian sperm, NKA alpha 4 is the main contributor to sperm motility, while the role of NKA alpha 1 remains unclear. Changing the affinity of ouabain for NKA did not affect male fertility.
JOURNAL OF MEMBRANE BIOLOGY
(2021)
Article
Cell Biology
Rajkumar Rajanathan, Claudia Vilaseca i Riera, Tina Myhre Pedersen, Christian Staehr, Elena V. Bouzinova, Jens Randel Nyengaard, Morten B. Thomsen, Hans Erik Botker, Vladimir V. Matchkov
Summary: This study investigated the expression of two isoforms (alpha(1) and alpha(2)) of the Na+,K+-ATPase in the cardiovascular system, and the contribution of the alpha(2)-isoform to cardiac contractility. The study found that hearts with the alpha(+/G301R)(2) mutation exhibited greater contractility and a stronger response to ouabain, which was associated with increased systolic work.
Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Genetics & Heredity
Jeff P. McDermott, September Numata, Gustavo Blanco
Summary: The study investigated the role of Atp1a4 in controlling sperm morphology, revealing that Atp1a4 plays a crucial role in maintaining the proper shape of the sperm flagellum through osmotic control and structurally related mechanisms. Atp1a4 knockout sperm exhibited a bend in the flagellum with cytoplasm dilation and fusion of the plasma membrane, as well as defects in axoneme and peri-axonemal structures. Additionally, Atp1a4 KO sperm showed abnormal response to osmotic challenge with decreased viability and reduced expression of key sperm structural proteins.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Rajkumar Rajanathan, Tina Myhre Pedersen, Halvor Osterby Guldbrandsen, Lenette Foldager Olesen, Morten B. Thomsen, Hans Erik Botker, Vladimir V. Matchkov
Summary: Heterozygous mice for the migraine-associated mutation (G301R) in the Na+,K+-ATPase alpha(2)-isoform have decreased expression of cardiovascular alpha(2)-isoform and exhibit a pro-contractile vascular phenotype associated with reduced cardiac efficiency. This study aims to assess the functional contribution of the alpha(2)-isoform to in vivo cardiovascular function and showed that the ouabain-induced vascular response was augmented in alpha(+/G301R)(2) mice, leading to suppressed cardiac function.
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.