Review
Plant Sciences
Lu Liu, Jun Liu, Ning Xu
Summary: Lectin receptor-like kinases (LecRKs) are located on the cell membrane and play diverse roles in environmental perception in higher plants. They are involved in plant development and response to abiotic and biotic stresses. This review summarizes the identified ligands of LecRKs in Arabidopsis, as well as discussing the posttranslational modification of these receptors in plant innate immunity and future research perspectives on plant LecRKs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Mari Ogawa-Ohnishi, Tomohide Yamashita, Mitsuru Kakita, Takuya Nakayama, Yuri Ohkubo, Yoko Hayashi, Yasuko Yamashita, Taizo Nomura, Saki Noda, Hidefumi Shinohara, Yoshikatsu Matsubayashi
Summary: Deciding whether to grow or divert energy to stress responses is a major trade-off for plants in fluctuating environments. Our study reveals that three leucine-rich repeat receptor kinases (LRR-RKs) act as direct receptors for PLANT PEPTIDE CONTAINING SULFATED TYROSINE (PSY)-family peptides, mediating the switching between two opposing pathways. Unlike other known LRR-RKs, PSY receptors (PSYRs) activate the expression of stress response transcription factors when the ligands are depleted. Loss of PSYRs leads to defects in plant tolerance to both biotic and abiotic stresses. This ligand-deprivation-dependent activation system allows plants to regulate stress responses in tissues near damaged sites where ligand production is impaired.
Article
Multidisciplinary Sciences
Zunyong Liu, Shuguo Hou, Olivier Rodrigues, Ping Wang, Dexian Luo, Shintaro Munemasa, Jiaxin Lei, Jun Liu, Fausto Andres Ortiz-Morea, Xin Wang, Kinya Nomura, Chuanchun Yin, Hongbo Wang, Wei Zhang, Keyan Zhu-Salzman, Sheng Yang He, Ping He, Libo Shan
Summary: Stomatal reopening in plants is dynamically regulated by the interaction between secreted peptides SCREWs and the receptor kinase NUT. This system counter-regulates the closure of stomata induced by abscisic acid and microbe-associated molecular patterns. SCREWs trigger the phosphorylation of key proteins involved in stomatal closure, leading to increased activity of kinase OST1 and reduced activity of anion channels. The SCREW-NUT system plays an important role in preventing uncontrolled stomatal closure and optimizing plant fitness.
Article
Biochemistry & Molecular Biology
Xiao Yu, Yingpeng Xie, Dexian Luo, Hai Liu, Marcos V. V. de Oliveira, Peipei Qi, Sung-Il Kim, Fausto Andres Ortiz-Morea, Jun Liu, Yafei Chen, Sixue Chen, Barbara Rodrigues, Bo Li, Shaowu Xue, Ping He, Libo Shan
Summary: Enabling and constraining immune activation is crucial for maintaining cellular homeostasis. In Arabidopsis, BTL2, an uncharacterized receptor kinase, senses the integrity of BAK1/SERK4 and promotes NLR-mediated phytocytokine signaling, leading to autoimmunity. BTL2 forms complexes with multiple phytocytokine receptors, connecting PRR and NLR-mediated immunity.
Review
Plant Sciences
Clare Breit-McNally, Bradley Laflamme, Racquel A. Singh, Darrell Desveaux, David S. Guttman
Summary: A key aspect of innate immunity in plants involves the recognition of pathogen effector virulence proteins by host Nucleotide-Binding Leucine-Rich Repeat Receptors (NLRs). The ZAR1 NLR has the remarkable ability to recognize at least six different families of effectors from two bacterial genera. This broad recognition is achieved through interactions with two families of Receptor-Like Cytoplasmic Kinases (RLCKs): ZED1-Related Kinases (ZRKs) and PBS1-Like Kinases (PBLs).
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Chang Liu, Hasi Yu, Aline Voxeur, Xiaolan Rao, Richard A. Dixon
Summary: Modifying lignin or cellulose in transgenic plants can induce defense responses and negatively affect growth, but bypassing endogenous pectin signaling pathways is required for engineering plant cell walls.
Review
Plant Sciences
Gara Romero-Hernandez, Manuel Martinez
Summary: The success of plant response to environmental stressors depends on regulatory networks and phosphorylation plays a key role in activating or deactivating proteins. Protein kinases are responsible for phosphorylation and play an important role in signal transmission. This review focuses on the contribution of protein kinases to herbivore-triggered responses in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Aboubakr Moradi, Mohamed El-Shetehy, Jordi Gamir, Tina Austerlitz, Paul Dahlin, Krzysztof Wieczorek, Markus Kunzler, Felix Mauch
Summary: The study showed that expressing Coprinopsis cinerea lectin 2 (CCL2) in Arabidopsis enhanced plant resistance against various nematodes and fungal pathogens, while also improving plant growth and biomass production. The mechanism of CCL2-mediated disease resistance enhancement depended on its fucoside-binding ability, and not on direct growth inhibition. Transcriptional induction of defense genes was significantly increased in CCL2-expressing plants compared to wild type plants, indicating the potential of fungal defense lectins in plant protection.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Subhankar Biswas, Raju Mondal, Akanksha Srivastava, Maitri Trivedi, Sunil Kumar Singh, Yogesh Mishra
Summary: The study investigated the role of legume lectin-like proteins (LLPs) in Arabidopsis thaliana under various abiotic stresses. Analysis of AtLLPs' chromosomal localization, gene structure, protein motif, peptide sequence, phylogeny, and sub-cellular localization was conducted, revealing different expression patterns in response to abiotic stresses. The presence of important cis-regulatory elements (CREs) in the promoter sequences of AtLLPs suggested their involvement in regulating abiotic stress responses, supported by the expressional correlation analysis with CREs cognate transcription factors (TFs).
Article
Food Science & Technology
Kai Kai Ma, Maija Greis, Jiakai Lu, Alissa A. Nolden, David Julian McClements, Amanda J. Kinchla
Summary: Consumers are increasingly moving towards a plant-based diet, but some are avoiding common plant proteins due to potential allergenicity. This article outlines the factors affecting the functional performance of plant proteins and highlights current methods to characterize their functionality. It proposes a series of analytical tests to better predict the performance of plant proteins in foods.
Article
Multidisciplinary Sciences
Laila Giordano, Valerie Allasia, Alexandra Cremades, Sophie Hok, Franck Panabieres, Beatrice Bailly-Maitre, Harald Keller
Summary: This study revealed a previously uncharacterized role of a plant receptor domain in the regulation of ER stress during infection.
Article
Cell Biology
Hwi Seong Jeon, Eunjeong Jang, Jinwoo Kim, Seu Ha Kim, Myoung-Hoon Lee, Myung Hee Nam, Yuki Tobimatsu, Ohkmae K. Park
Summary: This study reveals that autophagy modulates pathogen-induced lignin formation. Impaired autophagy affects lignin deposition and immune responses. Microscopy analysis shows colocalization between monolignols and autophagic vesicles. These findings suggest that lignification in plant immunity is achieved through autophagic membrane trafficking.
Article
Biochemistry & Molecular Biology
Guo Wen, Zhongyi Xie, Yao Yang, Yuxue Yang, Qigao Guo, Guolu Liang, Jiangbo Dang
Summary: In this study, researchers identified a gene called NpPP2-B10 that plays a crucial role in resistant tobacco plants. By transferring this gene into susceptible tobacco cultivars, they found that it promotes resistance to black shank disease. Further investigation showed that NpPP2-B10 is involved in the plant immune response through the ubiquitin protease pathway. These findings provide important insights into the mechanisms of tobacco resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Gan Ai, Hai Zhu, Xiaowei Fu, Jin Liu, Tianli Li, Yang Cheng, Yang Zhou, Kun Yang, Weiye Pan, Huanxin Zhang, Zishan Wu, Saiyu Dong, Yeqiang Xia, Yuanchao Wang, Ai Xia, Yiming Wang, Daolong Dou, Maofeng Jing
Summary: Recent studies have shown that plants activate endoplasmic reticulum stress-mediated immunity (ERSI) in response to pathogen infection, with the regulator NAC089 playing a crucial role in this process. NAC089 translocates from the endoplasmic reticulum to the nucleus via the Golgi apparatus, contributing positively to plant resistance against pathogens.
Article
Biochemistry & Molecular Biology
Zhonglong Guo, Zheng Kuang, Yongxin Zhao, Yang Deng, Hao He, Miaomiao Wan, Yihan Tao, Dong Wang, Jianhua Wei, Lei Li, Xiaozeng Yang
Summary: This study updated the PmiREN2.0 database with a large amount of new miRNA data, including predicted upstream transcription factors and annotation of miRNA targets, constructed a genome-wide regulatory network centered on miRNAs, built phylogenetic trees of conserved miRNA families, added design tools, and established a forum called 'PmiREN Community' for resource and new discovery sharing.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Plant Sciences
Nikita Sajeev, Anirban Baral, Antoine H. P. America, Leo A. J. Willems, Remy Merret, Leonie Bentsink
Summary: Arabidopsis thaliana seed germination undergoes extensive translational control at two critical phase transitions: hydration translational shift and germination translational shift (GTS). This study identified 30 seed specific RBPs and 22 dynamic RBPs during GTS, including stress granule markers, indicating seeds may quickly adapt mRNA translation in response to environmental changes. This research provides insight into the world of RBPs during seed germination and their potential regulatory role in this developmentally regulated process.
Article
Plant Sciences
Kaile Sun, Danny Schipper, Evert Jacobsen, Richard G. F. Visser, Francine Govers, Klaas Bouwmeester, Yuling Bai
Summary: The study found that genetic silencing of potato susceptibility genes can enhance resistance against late blight disease. Different mechanisms, including cell death responses and hormone-mediated signaling pathways, are involved in resistance mediated by different gene silencing.
HORTICULTURE RESEARCH
(2022)
Article
Chemistry, Applied
Peter R. Shewry, Antoine H. P. America, Alison Lovegrove, Abigail J. Wood, Amy Plummer, Jessica Evans, Hetty C. van den Broeck, Luud Gilissen, Roland Mumm, Jane L. Ward, Zsuzsan Proos, Petra Kuiper, C. Friedrich H. Longin, Annica A. M. Andersson, Jan Philip van Straaten, Daisy Jonkers, Fred Brouns
Summary: The study found that bread wheat flour contained higher concentrations of total dietary fiber and fructans, and yeast and sourdough fermentation could reduce the concentration of fructans, with yeast having a greater effect.
Review
Microbiology
Yan Wang, Rory N. Pruitt, Thorsten Nuernberger, Yuanchao Wang
Summary: Plant pathogens utilize various strategies to evade plant immunity, hindering the effective use of host genetic resistance for disease control. This Review discusses current knowledge on how microbial pathogens successfully infect plants by evading their immune responses and explores the potential for engineering crop resistance based on this knowledge.
NATURE REVIEWS MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Weizhen Wang, Fan Zhang, Sicong Zhang, Zhaolin Xue, Linfang Xie, Francine Govers, Xili Liu
Summary: This study reveals the critical role of the PcDHCR7 gene in sterol biosynthesis, mycelium development, and pathogenicity in Phytophthora capsici. The loss of PcDHCR7 leads to changes in gene expression and potentially compensatory modulation of the transcriptome.
Article
Biochemistry & Molecular Biology
Lan Wang, Hanmei Liu, Mingmei Zhang, Yu Ye, Lei Wang, Jinyi Zhu, Zhaodan Chen, Xiaobo Zheng, Yan Wang, Yuanchao Wang
Summary: Plant pattern recognition receptors (PRRs) are receptors that sense microbial invasion and activate immune responses. This study identified a glycoside hydrolase family 12 (GH12) protein Ps109281 secreted by Phytophthora sojae during infection. Ps109281 promotes P. sojae infection but does not induce cell death. It triggers immune responses by interacting with the receptor-like protein RXEG1. The loss of cell death induction is linked to a sequence polymorphism at the N-terminus.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jochem Bronkhorst, Kiki Kots, Djanick de Jong, Michiel Kasteel, Thomas van Boxmeer, Tanweer Joemmanbaks, Francine Govers, Jasper van der Gucht, Tijs Ketelaar, Joris Sprakel
Summary: This study reveals an actin-based mechanostat in Phytophthora infestans that controls the sharpness of the hyphal tip during penetration, allowing for efficient conversion of turgor into localized invasive pressures needed for host penetration.
Article
Plant Sciences
Ziwei Wang, Tingting Li, Xiaojiang Zhang, Jiashu Feng, Zhuting Liu, Weixing Shan, Matthieu H. A. J. Joosten, Francine Govers, Yu Du
Summary: Ubiquitin-like domain-containing proteins (UDPs) are involved in the ubiquitin-proteasome system. The Phytophthora infestans effector Pi06432 targets the potato StUDP protein, leading to the suppression of salicylic acid (SA)-related immunity. StUDP interacts with and destabilizes the 26S proteasome subunit StRPT3b, repressing proteasome activity. This destabilization also decreases the stability of the master transcription factor SARD1 and lowers SA content.
Article
Biotechnology & Applied Microbiology
Zongkuan Wang, Bo Yang, Wenyue Zheng, Lei Wang, Xingxing Cai, Jie Yang, Rongrong Song, Sen Yang, Yuyin Wang, Jin Xiao, Huiquan Liu, Yan Wang, Xiue Wang, Yuanchao Wang
Summary: This study found that Fusarium graminearum (FHB) is a devastating disease in wheat, causing substantial yield losses and mycotoxin contamination. Two GH12 proteins, Fg05851 and Fg11037, were found to have redundant roles in F. graminearum colonization and were recognized by the receptor-like protein RXEG1. Introducing RXEG1 into different wheat cultivars enhanced resistance to F. graminearum.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Zhaodan Chen, Fan Liu, Mengzhu Zeng, Lei Wang, Hanmei Liu, Yujing Sun, Lan Wang, Zhichao Zhang, Zhiyuan Chen, Yuanpeng Xu, Mingmei Zhang, Yeqiang Xia, Wenwu Ye, Suomeng Dong, Francine Govers, Yan Wang, Yuanchao Wang
Summary: In this study, scientists identified a receptor named REL that can recognize the extracellular protein ELicitin. REL is a receptor-like protein (RLP) that mediates plant resistance against Phytophthora by binding to ELicitin. The island domain (ID) within the leucine-rich repeat (LRR) domain of REL was found to be crucial for ELicitin recognition.
Article
Agronomy
Chia-Hui Hu, Francine Govers, Ignazio Carbone, Jean Beagle Ristaino
Summary: This study sequenced DNA from Phytophthora infestans and found that its population is highly diverse due to sexual reproduction and that oospores can overwinter in the soil. The study also revealed that nucleotide diversity and population mean mutation rates were higher in samples from organic fields and refuse piles than conventional fields for both nuclear and mitochondrial loci.
Article
Microbiology
Weizhen Wang, Zhaolin Xue, Linfang Xie, Xin Zhou, Fan Zhang, Sicong Zhang, Francine Govers, Xili Liu
Summary: Phytophthora is a genus of oomycetes that are destructive plant pathogens. They lack the ability to produce sterols but can utilize exogenous sterols for growth and development. This study identified four genes in P. capsici that play key roles in sterol signaling and regulate asexual reproduction and pathogenicity. It provides insights into the utilization of sterols by Phytophthora and explores their biological functions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Alison Lovegrove, Jack Dunn, Till K. Pellny, Jessica Hood, Amanda J. Burridge, Antoine H. P. America, Luud Gilissen, Ruud Timmer, Zsuzsan A. M. Proos-Huijsmans, Jan Philip van Straaten, Daisy Jonkers, Jane L. Ward, Fred Brouns, Peter R. Shewry
Summary: Five cultivars of bread wheat and spelt and three of emmer were grown in replicate randomised field trials on two sites for two years with different levels of nitrogen fertiliser. The analysis of wholemeal flours found that while the components overlapped between the cereal types, there were statistically significant differences in the contents of certain components. Emmer and spelt had higher contents of several beneficial components, but also contained higher levels of potentially harmful substances. Bread wheat had higher contents of certain types of fiber. The overall effects on health will depend on the quantity consumed and the composition of the diet.
Review
Cell Biology
Michiel Kasteel, Tijs Ketelaar, Francine Govers
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)