Article
Virology
Shufeng Liu, Chao-Kai Chou, Wells W. Wu, Binquan Luan, Tony T. Wang
Summary: This study presents a method to obtain stable cell clones without specific genes from SARS-CoV-2 RNA, providing a robust cell-based system for genetic and functional analyses of SARS-CoV-2 replication and the development of antiviral drugs.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Kyoko Saito, Kentaro Shimasaki, Masayoshi Fukasawa, Ryosuke Suzuki, Yuko Okemoto-Nakamura, Kaoru Katoh, Tomohiko Takasaki, Kentaro Hanada
Summary: This study established and characterized Vero cell lines carrying YFV replicons, and investigated the effects of various drugs on the persistent replication of the replicons. The results showed that several drugs could inhibit the replication of the replicons, providing evidence for the screening of antiviral drugs.
Article
Biotechnology & Applied Microbiology
Frazer J. T. Buchanan, Shucheng Chen, Mark Harris, Morgan R. Herod
Summary: Hepatitis E virus (HEV) is an emerging pathogen that causes over 20 million cases of acute hepatitis globally each year. This study investigated the role of cyclophilins A and B (CypA/B) in HEV replication and found that while CypA and CypB are not essential for HEV replication, silencing of CypB reduces replication of some HEV isolates in certain cells. The dependence on cyclophilins for HEV replication appears to be genotype- and sequence-specific.
JOURNAL OF GENERAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Yaqiong Yang, Yu Sun, Rong Hu, Jia Yan, Ziheng Wang, Wenlong Li, Hong Jiang
Summary: This study investigated the regulatory mechanism of epidermal growth factor receptor (EGFR) on microglial activation induced by morphine in BV-2 cells. The results showed that morphine treatment increased CD11b expression, EGFR phosphorylation, ERK1/2 activation, cell migration, and production of proinflammatory cytokines, which were inhibited by blocking the EGFR signaling pathway with AG1478. These findings suggest that the EGFR/ERK signaling pathway could be a novel pharmacological strategy to attenuate morphine tolerance by suppressing microglial activation.
Review
Biochemistry & Molecular Biology
Jason M. Ridlon, Steven L. Daniel, H. Rex Gaskins
Summary: Bile acids are derived from cholesterol and function as detergents to solubilize lipids and remove cholesterol. They also act as signaling molecules in various tissues. The structures of bile acids were established in the early 20th century, and research has since differentiated primary from secondary bile acids. The formation of bile acids involves a multi-step, bifurcating pathway.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Multidisciplinary Sciences
Qiwei Jiang, Deyu Zhang, Juan Liu, Chaoyang Liang, Ronghui Yang, Cheng Zhang, Jun Wu, Jing Lin, Tianxing Ye, Lihua Ding, Jianbin Li, Shan Gao, Binghui Li, Qinong Ye
Summary: The EGFR-RAS-ERK pathway is crucial in cancer development, and the assembly of its signaling complexes is not well understood. HPIP was found to interact with all components of this pathway and form multiple complexes. Knockout or knockdown of HPIP inhibited complex formation, signaling activation, glycolysis, and cancer cell growth. High HPIP expression correlated with worse clinical outcomes in lung cancer patients. These findings shed light on signaling complex formation and regulation, and suggest HPIP as a potential therapeutic target for EGFR-RAS-ERK dysregulated cancers.
Article
Biochemistry & Molecular Biology
Jin Lee, Eun Mi Hong, Jung Han Kim, Jung Hee Kim, Jang Han Jung, Se Woo Park, Dong Hee Koh, Hyun Joo Jang
Summary: The study demonstrated the inhibitory effects of UDCA on proliferation, induction of apoptosis, and suppression of invasiveness in bile duct cancer cells. UDCA activates p53, blocks DCA-induced EGFR-ERK signaling and COX-2, inhibits DCA-induced PI3K-AKT signaling, and suppresses invasiveness in these cancer cells.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Oncology
Afeez Adekunle Ishola, Chian-Shiu Chien, Yi-Ping Yang, Yueh Chien, Aliaksandr A. Yarmishyn, Ping-Hsing Tsai, Jerry Chieh-Yu Chen, Po-Kuei Hsu, Yung-Hung Luo, Yuh-Min Chen, Kung-Hou Liang, Yuan-Tzu Lan, Teh-Ia Huo, Hsin- Ma, Ming-Teh Chen, Mong-Lien Wang, Shih-Hwa Chiou
Summary: Lung cancers, particularly non-small cell lung cancers, have a poor prognosis. A circular noncoding RNA called C190 has been identified as a negative prognostic biomarker for lung cancer, and this study explores its mechanistic function and potential as a therapeutic target for NSCLC.
Article
Veterinary Sciences
Huan Wang, Dingxiang Liu, Yingjie Sun, Chunchun Meng, Lei Tan, Cuiping Song, Xusheng Qiu, Weiwei Liu, Chan Ding, Liao Ying
Summary: Understanding virus-cell interactions is crucial in combating viral infections. The MEK-ERK1/2 signaling pathway facilitates IBV infection by promoting cell survival, while the induction of DUSP6 forms a negative regulation loop to limit ERK1/2 signaling, leading to increased apoptosis and reduced viral load. Targeting components of the ERK pathway for antiviral drug development shows promise in controlling viral replication.
VETERINARY RESEARCH
(2021)
Article
Oncology
Renba Liang, Xiaodong Zhu
Summary: UC2288 inhibited the growth of NPC by suppressing the EGFR/ERK pathway and may be a promising therapeutic option for NPC.
Article
Medicine, Research & Experimental
Jinming Xu, Yan Wang, Jiahao Jiang, Cong Yin, Bentao Shi
Summary: ADAM12 is significantly upregulated in clear cell renal cell carcinoma (ccRCC) and is associated with poor prognosis. ADAM12 promotes ccRCC cell proliferation, migration, and invasion by activating the EGFR/ERK signaling pathway, inducing epithelial-mesenchymal transition (EMT), and upregulating target genes.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Cell Biology
Wei Jia, Meilin Wei, Cynthia Rajani, Xiaojiao Zheng
Summary: The gut microbiota plays a significant role in glucose and lipid metabolism by regulating bile acid metabolism and affecting multiple signaling pathways. Bile acids are synthesized in the liver through two distinct pathways, influencing cholesterol and lipid catabolism and activating various signaling pathways. Modulating bile acid synthesis and composition could be a novel approach for developing therapies for metabolic diseases.
Article
Medicine, Research & Experimental
Na Yang, Runbin Sun, Xiaoli Zhang, Jing Wang, Lulu Wang, Huaijun Zhu, Man Yuan, Yifan Xu, Chun Ge, Jun He, Min Wang
Summary: Non-alcoholic steatohepatitis (NASH) is a serious liver disorder characterized by hepatic steatosis and inflammation. The induction of NAMPT/NAD+/SIRT1 axis can effectively alleviate liver inflammation in NASH mice and modulate the synthesis of bile acids (BAs). This may offer a potential therapeutic strategy for NASH or its complications related to BAs.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Chuanren Zhou, Xiaomin Pan, Lei Huang, Tianzhen Wu, Tiantian Zhao, Jie Qi, Jiamin Wu, Alan Vengai Mukondiwa, Yuli Tang, Yongde Luo, Qi Tu, Zhifeng Huang, Jianlou Niu
Summary: Cholestasis is a pathological process that leads to liver injury and diseases, and the liver-secreted hormone FGF21 acts as a signaling molecule to regulate the synthesis of bile acids, thereby alleviating cholestasis-induced damage.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Gastroenterology & Hepatology
Laura Conde de la Rosa, Carmen Garcia-Ruiz, Carmen Vallejo, Anna Baulies, Susana Nunez, Maria J. Monte, Jose J. G. Marin, Lucia Baila-Rueda, Ana Cenarro, Fernando Civeira, Josep Fuster, Juan C. Garcia-Valdecasas, Joana Ferrer, Michael Karin, Vicent Ribas, Jose C. Fernandez-Checa
Summary: The study revealed an unrecognized role of STARD1 in the pathogenesis of HCC, promoting the synthesis of primary BAs through the mitochondrial pathway, which stimulate hepatocyte pluripotency, self-renewal, and inflammation in tumour-initiated stem-like cells (TICs).
JOURNAL OF HEPATOLOGY
(2021)