Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 59, Issue 3, Pages 424-433Publisher
WILEY
DOI: 10.1002/mnfr.201400674
Keywords
Absorption; Broccoli sprout; Chemoprevention; Excretion; Sulforaphane
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Funding
- Linus Pauling Institute
- Center for Genome Research and Biocomputing Core Facilities
- Mass Spectrometry Lab at Oregon State University
- National Institutes of Health [CA090890, CA122906]
- National Institute of Environmental Health Sciences [P30 ES000210]
- Oregon Agricultural Experimental Station
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Scope: Sulforaphane (SFN), an isothiocyanate derived from crucifers, has numerous health benefits. SFN bioavailability from dietary sources is a critical determinant of its efficacy in humans. A key factor in SFN absorption is the release of SFN from its glucosinolate precursor, glucoraphanin, by myrosinase. Dietary supplements are used in clinical trials to deliver consistent SFN doses, but myrosinase is often inactivated in available supplements. We evaluated SFN absorption from a myrosinase-treated broccoli sprout extract (BSE) and are the first to report effects of twice daily, oral dosing on SFN exposure in healthy adults. Methods and results: Subjects consumed fresh broccoli sprouts or the BSE, each providing 200 mu mol SFN daily, as a single dose and as two 100-mu mol doses taken 12 h apart. Using HPLC-MS/MS, we detected similar to 3 x higher SFN metabolite levels in plasma and urine of sprout consumers, indicating enhanced SFN absorption from sprouts. Twelve-hour dosing retained higher plasma SFN metabolite levels at later time points than 24-hour dosing. No dose responses were observed for molecular targets of SFN (i.e. heme oxygenase-1, histone deacetylase activity, p21). Conclusion: We conclude that the dietary form and dosing schedule of SFN may impact SFN absorption and efficacy in human trials.
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