Article
Pharmacology & Pharmacy
John D. Imig, Md Abdul Hye Khan, Anna Stavniichuk, Wojciech K. Jankiewicz, Samaneh Goorani, Michael M. Yeboah, Ashraf El-Meanawy
Summary: The incidence of ureter obstruction is increasing and recovery from this kidney injury can lead to long-term consequences such as an increased risk for salt-sensitive hypertension. A study using a RUUO model found that mice with unilateral ureteral obstruction had elevated blood pressure and decreased sodium excretion, along with renal tubular injury, inflammation, decreased vascular density, and renal fibrosis. These findings highlight the importance of addressing ureter obstruction and its potential impact on sodium excretion and hypertension.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Immunology
Xiaofei Li, Jing Zhao, Said Movahedi Naini, Gianmarco Sabiu, Stefan G. Tullius, Su Ryon Shin, Jonathan S. Bromberg, Paolo Fiorina, George C. Tsokos, Reza Abdi, Vivek Kasinath
Summary: The study revealed that after unilateral ureteral obstruction in the kidney, fibroblastic reticular cells (FRCs) in the kidney-draining lymph node also become fibrogenic, contributing to fibrosis that closely mirrors changes in the kidney. Additionally, immune cell populations in the affected lymph node were significantly higher compared to the unaffected contralateral kidney-draining lymph node. This suggests a possible mechanism for fibrosis involving the TGF beta/TGF beta R signaling pathway, and future studies are needed to delve deeper into this connection between FRCs and lymph node fibrosis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Stine Julie Tingskov, Michael Schou Jensen, Casper-Emil Tingskov Pedersen, Isabela Bastos Binotti Abreu de Araujo, Henricus A. M. Mutsaers, Rikke Norregaard
Summary: The study found that the selective estrogen receptor modulator tamoxifen has a mitigating effect on renal fibrosis in female, male, and ovariectomized female rats, as well as demonstrating an anti-fibrotic effect in human kidney slices. Gender differences may play a role in drug treatment response.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Plant Sciences
Yu-Teng Chang, Mu-Chi Chung, Chi-Hao Chang, Kuan-Hsun Chiu, Jeng-Jer Shieh, Ming-Ju Wu
Summary: This study evaluated the effects of protocatechuic aldehyde (PCA) on renal fibrosis. The results showed that PCA could inhibit TGF-beta 1-induced fibrosis and EMT, suggesting it as a potential strategy for preventing organ fibrosis in clinics.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Dwi Cahyani Ratna Sari, Santosa Budiharjo, Husnari Afifah, Destantry Jasmin, Orisativa Kokasih, Tiara Gitami Putri, Karina Arifiani, Wiwit Ananda Wahyu Setyaningsih, Nur Arfian
Summary: CeA treatment in UUO mice model effectively ameliorated kidney fibrosis by reducing interstitial fibrosis area, suppressing organ injury, and regulating the expression of various genes, which resulted in decreased inflammation and mesenchymal transition.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ana Karina Aranda-Rivera, Alfredo Cruz-Gregorio, Omar Emiliano Aparicio-Trejo, Edilia Tapia, Laura Gabriela Sanchez-Lozada, Fernando Enrique Garcia-Arroyo, Isabel Amador-Martinez, Marisol Orozco-Ibarra, Francisca Fernandez-Valverde, Jose Pedraza-Chaverri
Summary: Sulforaphane (SFN) confers protection against unilateral ureteral obstruction (UUO)-induced kidney injury by targeting mitochondrial biogenesis and improving lipid metabolism.
Article
Biochemistry & Molecular Biology
Ariadna Jazmin Ortega-Lozano, Alexis Paulina Jimenez-Uribe, Ana Karina Aranda-Rivera, Leopoldo Gomez-Caudillo, Emmanuel Rios-Castro, Edilia Tapia, Belen Bellido, Omar Emiliano Aparicio-Trejo, Laura Gabriela Sanchez-Lozada, Jose Pedraza-Chaverri
Summary: The study examined the impact of UUO at different time points on the mitochondrial proteome of rat kidneys, revealing changes in proteins related to oxidative phosphorylation, the tricarboxylic acid cycle, and fatty acid metabolism, all of which are associated with bioenergetics during ON.
Article
Environmental Sciences
Yanping Li, Yanfen Chai
Summary: In this study, the role of circ_0040994 in lipopolysaccharide-induced acute kidney injury was investigated. The results showed that circ_0040994 was upregulated in the serum of septic AKI patients. Silencing circ_0040994 could enhance cell viability and inhibit cell apoptosis, inflammation, and oxidative stress. Furthermore, circ_0040994 acted as a sponge for miR-17-5p to regulate the levels of TRPM7, and miR-17-5p could alleviate LPS-induced cell injury by suppressing TRPM7.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Cell Biology
Yi-Hsien Hsieh, Wen-Chien Wang, Tung-Wei Hung, Chu-Che Lee, Jen-Pi Tsai
Summary: CCL17, a chemotactic cytokine produced by macrophages, is elevated in chronic kidney disease patients and negatively correlated with renal function, making it a potentially useful biomarker for predicting the development of advanced CKD. In vitro and in vivo experiments demonstrate that CCL17 promotes renal fibrosis by inducing epithelial-mesenchymal transition and ECM accumulation.
Article
Pharmacology & Pharmacy
Qiongdan Hu, Bingwen Zhu, Guoqiang Yang, Jian Jia, Honglian Wang, Ruizhi Tan, Qiong Zhang, Li Wang, Fahsai Kantawong
Summary: In this study, the researchers found that Calycosin, a phytoestrogen, can enhance the antifibrotic activity of bone marrow-derived mesenchymal stem cells (MSCs) in primary tubular epithelial cells and a mouse model of unilateral ureteral obstruction. It achieves this by inhibiting necroptosis induced by TGF-b1/TNF-a/TNFR1 signaling.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alexis Paulina Jimenez-Uribe, Belen Bellido, Omar Emiliano Aparicio-Trejo, Edilia Tapia, Laura Gabriela Sanchez-Lozada, Jose Antonio Hernandez-Santos, Francisca Fernandez-Valverde, Estefani Yaquelin Hernandez-Cruz, Marisol Orozco-Ibarra, Jose Pedraza-Chaverri
Summary: This study evaluated the time course of mitochondrial mass and bioenergetics alterations induced by a unilateral ureteral obstruction (UUO), a widely used model to study kidney fibrosis induction and progression mechanisms. The results showed a marked reduction in mitochondrial oxidative phosphorylation (OXPHOS) and mitochondrial mass during days 7 to 28 of obstruction, suggesting a key role of mitochondrial biogenesis and mitophagy induction in the impairment of OXPHOS capacity during fibrotic progression.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Mei Ying Xuan, Shang Guo Piao, Jun Ding, Qi Yan Nan, Mei Hua Piao, Yu Ji Jiang, Hai Lan Zheng, Ji Zhe Jin, Can Li
Summary: Dapagliflozin alleviates renal fibrosis induced by unilateral ureteral obstruction (UUO) by inhibiting RIP1-RIP3-MLKL-mediated necroinflammation through Wnt3 alpha/beta-catenin/GSK-3 beta signaling.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana Karina Aranda-Rivera, Alfredo Cruz-Gregorio, Omar Emiliano Aparicio-Trejo, Ariadna Jazmin Ortega-Lozano, Jose Pedraza-Chaverri
Summary: In the UUO model, overproduction of ROS leads to alterations in signaling pathways, promoting inflammation, oxidative stress, and apoptosis, contributing to fibrosis development. Impairment of mitochondrial metabolism is also involved in this process.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Fayez T. Hammad, Loay Lubbad, Suhail Al-Salam, Javed Yasin, Mohamed Fizur Nagoor Meeran, Shreesh Ojha, Waheed F. Hammad
Summary: Both ureteral obstruction and hypertension have significant impacts on kidney functions. Reversible ureteral obstruction in hypertensive rats showed exaggerated alterations in renal functions and other parameters of renal injury compared to normotensive rats.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Integrative & Complementary Medicine
Shiguang Yang, Kui Zhao, Xiaodong Yang, Chungen Xing
Summary: This study discovered that circ-PITHD1 suppressed CRC progression by inhibiting glycolysis via the miR-590-5p/HK2 axis.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Genetics & Heredity
Zijian Zhu, Biqing Chen, Ren Na, Wan Fang, Wenxia Zhang, Qin Zhou, Shanbi Zhou, Han Lei, Ailong Huang, Tingmei Chen, Dongsheng Ni, Yuping Gu, Jianing Liu, Yi Rao, Fang Fang
Summary: The study explored the genetic basis of two subtypes of the Ebbinghaus illusion in humans, finding moderate heritability for EI overestimation but not for EI underestimation. Through quantitative genomic analyses, a number of SNPs and genes associated with EI overestimation were identified, providing consistent evidence for a substantial genetic basis of the Ebbinghaus illusion.
JOURNAL OF HUMAN GENETICS
(2021)
Article
Cell Biology
Liang Ye, Xinyuan Zhang, Qin Zhou, Bin Tan, Hao Xu, Qin Yi, Liang Yan, Min Xie, Yin Zhang, Jie Tian, Jing Zhu
Summary: Activation of AMPK improves the maturation of hiPSC-CMs by increasing the expression of CM-specific markers, improving myocardial structure, enhancing mitochondrial function, and promoting mitochondrial fusion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Hua Xia, Yamin Liu, Lei Xu, Huajie Mao, Qin Zhou, Yajun Xie
Summary: The study demonstrated that A1CF overexpression facilitated cell migration in renal cell carcinoma, while A1CF deficiency downregulated the expression of genes like SMAD3. A1CF promoted the nuclear translocation of SMAD3 and interacted with it.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2021)
Article
Cell & Tissue Engineering
Xinyuan Zhang, Liang Ye, Hao Xu, Qin Zhou, Bin Tan, Qin Yi, Liang Yan, Min Xie, Yin Zhang, Jie Tian, Jing Zhu
Summary: The study explored the role of NRF2 in the maturation of hiPSC-CMs by analyzing morphological and functional changes related to mitochondria and cell maturation through knock-down and activation of NRF2. The results showed that NRF2 inhibition led to retardation of cell maturation, while NRF2 activation resulted in a more mature hiPSC-CMs phenotype. Bioinformatics analysis indicated that NRF2 not only regulates metabolism-related genes but also activates the expression of myocardial ion channels.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Medicine, Research & Experimental
Jianhua Pan, Meng Ma, Li Qin, Zhongkui Kang, Dickson Adah, Zhu Tao, Xiaofen Li, Linglin Dai, Siting Zhao, Xiaoping Chen, Qin Zhou
Summary: The study found that Plasmodium infection inhibited tumor growth and increased survival rate in a murine triple negative breast cancer model by enhancing CD8+ T cell responses. This suggests a potential novel strategy for the treatment of triple negative breast cancer through induction of antitumor immune responses mediated by CD8+ T cells.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Electrochemistry
Feiyun Cui, Zhiru Zhou, Bin Qu, Qin Zhou, H. Susan Zhou
Summary: In this study, a low-cost and multiplexing electrochemical (EC) device named EC 6-well plate was developed. The device was fabricated using a non-photolithography method and discussed the miniaturization of reference electrodes. An immunosensor for toxin B detection was successfully developed using the EC 6-well plate, showing promising stability.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Review
Chemistry, Analytical
Jianlei Liu, Haijie Ji, Xiaoyan Lv, Chijia Zeng, Heming Li, Fugang Li, Bin Qu, Feiyun Cui, Qin Zhou
Summary: This mini review provides an overview of laser-induced graphene (LIG) and its applications in medical sensing devices. Various methods for preparing graphene are compared and discussed. The applications of LIG in biochemical sensors and wearable electronics for medical applications are highlighted. Insights into current challenges and prospects for LIG-based medical sensing devices are also presented.
Article
Biophysics
Feiyun Cui, Xiaoqun Shen, Bo Cao, Haijie Ji, Jianlei Liu, Xiwei Zhuang, Chijia Zeng, Bin Qu, Shunbo Li, Yi Xu, Qin Zhou
Summary: A biosensor integrated with mannose nano-surface was developed for the identification and adhesive strength evaluation of bacteria. S. typhimurium and E. coli JM109 (type 1 pili) were captured by the mannose nano-surface. The species of captured bacteria were identified using SERS spectra and PLS-DA. The binding affinities of the captured bacteria to the nano-surface were obtained and the results showed that S. typhimurium had a stronger adhesive strength. Normalized impedance change (NIC) was found to be positively correlated with binding affinities, indicating it can be used as an indicator for the adhesive strength of bacteria. The developed biosensor can provide both qualitative and quantitative information of selective recognition between bacteria and mannose.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Electrochemistry
Xiwei Zhuang, Xueqiong Yang, Bo Cao, Haiming Sun, Xiaoyan Lv, Chijia Zeng, Fugang Li, Bin Qu, H. Susan Zhou, Feiyun Cui, Qin Zhou
Summary: The CRISPR/Cas system has gained attention for its gene-editing capabilities. The reported trans-cleavage activity of Cas proteins has increased the system's diagnostic ability. When applied in electrochemical biosensors, the CRISPR/Cas system provides high specificity, low detection limits, and meets point-of-care testing requirements. This review introduces the history, advantages, and applications of EC biosensors based on the CRISPR/Cas system, and proposes future prospects.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Anatomy & Morphology
Xin Yan, Dayu Tian, Rui Peng, Lei Chen, Qingling He, Qin Zhou, Qianyin Li
Summary: This study reveals the role of TTC36, a chaperone for heat shock protein 70, in acute kidney injury. The down-regulation of TTC36 is negatively correlated with the severity of kidney injury, and it protects renal tubular cells from cisplatin-induced apoptosis by maintaining mitochondrial potential and expression of mitochondrial autophagy-related genes.
Article
Oncology
Qin Tang, Weichu Liu, Dan Jiang, Junying Tang, Qin Zhou, Jing Zhang
Summary: This study compared the perioperative and survival outcomes of robotic-assisted surgery, traditional laparoscopy, and laparotomy approaches in ovarian cancer. The results showed that compared to laparotomy, robotic-assisted laparoscopy and laparoscopy had shorter hospital stays, decreased blood loss, and lower rates of complications and transfusion. There were no significant differences in 5-year overall survival among the three surgical approaches.
JOURNAL OF ONCOLOGY
(2022)
Article
Genetics & Heredity
Huajie Mao, Ya Zhao, Li Lei, Yanxia Hu, Hangrui Zhu, Runzhi Wang, Dongsheng Ni, Jianing Liu, Lei Xu, Hua Xia, Zaikuan Zhang, Meng Ma, Zheng Pan, Qin Zhou, Yajun Xie
Summary: SELS is highly expressed in clear cell renal cell carcinoma (ccRCC) and associated with multiple pathological features. SELS overexpression promotes cell proliferation and inhibits apoptosis, while silence of SELS has the opposite effect. Mechanistically, SELS activates AKT/GSK3 beta/NF-kappa B signaling pathway to enhance cell proliferation and inhibit apoptosis, and stabilizes c-Myc by preventing ubiquitin-proteasome-mediated degradation. Furthermore, SELS inhibits ccRCC cell migration likely through repressing epithelial-mesenchymal transition (EMT).
Article
Biochemistry & Molecular Biology
Xin Yan, Rui Peng, Yilu Ni, Lei Chen, Qingling He, Qianyin Li, Qin Zhou
Summary: This study revealed the association between TTC36 reduction and CKD induced by unilateral ureteral obstruction (UUO), and showed that ablation of TTC36 attenuated tubular injury and subsequent EMT. TTC36 overexpression promoted EMT in TGF-I31-induced HK2 cells. Moreover, TTC36 elevated the protein expression of CEBPB, which regulated TGF-I3/SMAD3 signaling.
Article
Cell Biology
Juan Chen, Xiaoliang Hua, Heying Chen, Xiangmin Qiu, Haibing Xiao, Shengdong Ge, Chaozhao Liang, Qin Zhou
Summary: PKMYT1 expression is closely associated with the pathological features of ccRCC and may participate in the tumorigenesis and progression of ccRCC by affecting cell cycle-related pathways and the EMT process. Knockdown of PKMYT1 in vitro can reduce the growth, migration, and invasion ability of RCC cells, promote cell apoptosis, and prevent the occurrence of the EMT phenotype.
Article
Pharmacology & Pharmacy
Xie Sujuan, Zhao Limei, Xiao Di, Zhou Qin
Summary: This study assessed the effects of sFlt-1, IGFBP-3, and PAPP-A on missed abortion, finding differences in their expression levels in different abortion situations. The joint detection of these markers showed high accuracy and sensitivity, but lower specificity. Further realistic approaches, including alternative sampling methods and large populations, are needed for detecting missed abortion.
LATIN AMERICAN JOURNAL OF PHARMACY
(2021)