Nocardioides soli sp nov., a carbendazim-degrading bacterium isolated from soil under the long-term application of carbendazim

The taxonomic status of a carbendazim-degrading strain, mbc-2(T), isolated from soil under the long-term application of carbendazim in China was determined by means of a polyphasic study. The cells were Gram-stain-positive, motile and rod-shaped. Strain mbc-2(T) grew optimally at pH 7.0, 30-35 degrees C and in the presence of 1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain mbc-2(T) fell within the genus Nocardioides, forming a coherent cluster with the type strain of Nocardioides hankookensis, with which it exhibited 16S rRNA gene sequence similarity values of 97.9%. The chemotaxonomic properties of strain mbc-2(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on LL-2,6-diaminopimelic acid, the predominant menaquinone was MK-8 (H-4) and the major fatty acid was iso-C-16:0. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unknown phospholipids and an unknown aminolipid. The DNA G+C content was 72 mol%. Strain mbc-2(T) exhibited DNA DNA relatedness values of 12.5 +/- 1.5%, 23.7 +/- 2.7% and 26.3 +/- 3.2% with respect to Nocardioides hankookensis DS-30(T), Nocardioides aquiterrae GW-9(T) and Nocardioides pyridinolyticus OS4(T). On the basis of the data obtained, strain mbc-2(T). represents a novel species of the genus Nocardioides, for which the name Nocardioides soli sp. nov. is proposed. The type strain is mbc-2(T) (=KACC 17152(T) =CCTCC AB 2012934(T)).