Article
Biotechnology & Applied Microbiology
Huseyin Basim, Esin Basim, Huseyin Tombuloglu, Turgay Unver
Summary: The transcriptome responses of Cmm-resistant and -susceptible tomato lines were compared after inoculation with Clavibacter michiganensis subsp. michiganensis. Differentially expressed genes were identified, with susceptible lines showing more genes involved in biological processes and molecular functions, while resistant lines exhibited distinct defense response and stress-related genes upon inoculation. This study provides insights for molecular breeding studies aiming to control the pathogen in tomato.
Article
Horticulture
Eva Sanchez-Hernandez, Vicente Gonzalez-Garcia, Ana Palacio-Bielsa, Belen Lorenzo-Vidal, Laura Buzon-Duran, Jesus Martin-Gil, Pablo Martin-Ramos
Summary: The aim of this study was to characterize the bioactive constituents in Ginkgo biloba extracts and test their activity against phytopathogenic bacteria. The leaf extract showed bacteriostatic activity and protected tomato and pea plants from bacterial pathogens.
Article
Microbiology
Dario Arizala, Shefali Dobhal, Anne M. Alvarez, Mohammad Arif
Summary: This study assessed the taxonomic position of Clavibacter michiganensis subspecies and clarified the taxonomy of other Clavibacter strains. Based on genomic sequencing and computational methods, it proposed raising C. michiganensis subspecies to a new species, Clavibacter californiensis, and merging C. michiganensis subsp. chilensis and C. michiganensis subsp. phaseoli into a new species, Clavibacter phaseoli.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Plant Sciences
Naoki Yokotani, Yoshinori Hasegawa, Masaru Sato, Hideki Hirakawa, Yusuke Kouzai, Yoko Nishizawa, Eiji Yamamoto, Yoshiki Naito, Sachiko Isobe
Summary: Bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis is an economically important disease. The host defense response to Cmm infection in tomato mainly involves the overexpression of genes related to defense response, phosphorylation, and hormone signaling, with some resistance genes being induced post-infection, indicating SA signaling may regulate Cmm-responsive genes.
Article
Plant Sciences
Ruchi Tripathi, K. Vishunavat, Rashmi Tewari
Summary: Tomato bacterial canker caused by Clavibacter michiganensis subsp michiganensis (Cmm) is an emerging disease in the Northern hill region of India. This study investigated the presence and spread of Cmm, and identified the cause of bacterial canker using conventional, serological, and molecular diagnostic methods. The results showed a Cmm incidence of 19.57% and disease severity of 16.11% in the region. Tomato seeds were found to be a potential means of quarantine pest spread.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2022)
Article
Microbiology
Miryam Valenzuela, Marianela Gonzalez, Alexis Velasquez, Fernando Dorta, Ivan Montenegro, Ximena Besoain, Francisco Salva-Serra, Daniel Jaen-Luchoro, Edward R. B. Moore, Michael Seeger
Summary: This study characterized nine strains of Clavibacter michiganensis subsp. michiganensis isolated in Chile, revealing differences in virulence. Two highly virulent strains showed increased cellulase activity, while a strain causing mild symptoms had no cellulase activity. PCR and gene sequence analysis indicated correlations between disease severity differences and the presence/absence of specific genes. Deletions in virulence genes were also observed in the Chilean strains.
Article
Chemistry, Multidisciplinary
Gabriel Marcelino-Perez, Roberto Ruiz-Medrano, Salvador Gallardo-Hernandez, Beatriz Xoconostle-Cazares
Summary: This study evaluated the antimicrobial activity of mesoporous silica nanoparticles loaded with human beta-defensin-2 and its mutants against Clavibacter michiganensis subsp. michiganensis. The results showed that the MSN loaded with AMPs inhibited the growth of Cmm and protected AMPs from enzymatic degradation.
Article
Biochemical Research Methods
Yoko Takishita, Sowmyalakshmi Subramanian, Alfred Souleimanov, Donald L. Smith
Summary: This study investigated the interactions between Pseudomonas entomophila 23S and Clavibacter michiganensis subsp. michiganensis (Cmm) at the proteomic level. The results showed that P. entomophila 23S produced a higher amount of anti-Cmm compound when co-cultured with Cmm, but its growth was negatively affected by the presence of Cmm.
JOURNAL OF PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Deborah Kim, Alexander S. Shashkov, Andrey S. Dmitrenok, Nataliya Potekhina, Sof'ya N. Senchenkova, Lubov Dorofeeva, Lyudmila Evtushenko, Elena M. Tul'skaya
Summary: The cell wall of Clavibacter michiganensis subsp. michiganensis VKM Ac-1403(T) has been found to contain two polysaccharides, with chemical and NMR spectroscopic methods used to establish their structures. The newly obtained data on the cell wall composition of this plant pathogenic species can enhance understanding of the molecular mechanisms involved in plant colonization and infection.
CARBOHYDRATE RESEARCH
(2021)
Article
Agronomy
Meral Yilmaz, Omur Baysal, Ragip Soner Silme
Summary: This study evaluated the efficacy of seed film coating with Origanum vulgare essential oil on tomatoes against Clavibacter michiganensis subsp. michiganensis. Results showed that carvacrol, the major component of O. vulgare essential oil, inhibited the growth of pathogenic bacteria and had a strong interaction with a specific bacterial protein. The study suggests a potential method for managing Cmm disease and preventing its transmission through seeds.
PLANT PROTECTION SCIENCE
(2021)
Article
Horticulture
Caterina Morcia, Isabella Piazza, Roberta Ghizzoni, Valeria Terzi, Ilaria Carrara, Giovanni Bolli, Giorgio Chiusa
Summary: In this study, a method based on chip digital PCR (cdPCR) was developed to identify and quantify the important bacterial pathogens Clavibacter michiganensis subsp. michiganensis (Cmm) and Ralstonia solanacearum (Rs) in tomatoes. The assays were effective in detecting and quantifying the bacteria in pure samples as well as naturally contaminated or spiked tomato samples. The cdPCR assays proposed can be used for rapid and accurate detection of these high-risk quarantine bacteria to prevent the spread of pathogens and occurrence of diseases in other areas.
Article
Plant Sciences
Dimitris Malliarakis, Marianthi G. Pagoulatou, Evaggelia Mpalantinaki, Emmanouil Trantas, Filippos Ververidis, Dimitrios E. Goumas
Summary: Through multi-locus sequencing analysis, it was found that the pathogen of tomato bacterial canker in Greece has genetic variability, but the classification of strains does not match the geographical distribution, likely due to the introduction of infected propagating material. However, strains isolated from the same area in different years belong to the same group, indicating that the pathogen can remain active between growing seasons. This study provides important epidemiological information on disease outbreaks and the spread of Cmm in Greece.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Microbiology
Hwajin Jang, Sang Tae Kim, Mee Kyung Sang
Summary: The water extracts of strains H8-1 and K203 can control the proliferation of Clavibacter michiganensis subsp. michiganensis (Cmm) and reduce wilting in tomato plants caused by Cmm. These extracts inhibit the expression of certain Cmm genes, increase antioxidant enzyme activity in plants, and enhance soil enzyme activity, promoting plant growth.
Article
Plant Sciences
Isil Temel, Mesude Figen Doenmez, Esat Temtek
Summary: Essential oils obtained from five different plants were analyzed for their chemical components and antibacterial activity against C. michiganensis. The results showed that all of the essential oils demonstrated varying levels of antibacterial activity, with T. migricus exhibiting the highest activity. This suggests that essential oils can be used as a natural alternative to synthetic pesticides.
JOURNAL OF ESSENTIAL OIL BEARING PLANTS
(2023)
Article
Agronomy
Hatice Filiz Boyaci, Aylin Kabas, Yesim Aysan, Jaime Prohens
Summary: Resistance sources to Clavibacter michiganensis subsp. michiganensis (Cmm) in eggplants were identified, and the genetic control of this resistance was investigated through a series of experiments with different genotypes. The results indicate a dominant and a recessive gene model underlying the resistance to Cmm, providing valuable information for breeding programs aiming to enhance resistance in eggplants.
PLANT PROTECTION SCIENCE
(2021)
Article
Plant Sciences
Jarred Yasuhara-Bell, Fulya Baysal-Gurel, Sally A. Miller, Anne M. Alvarez
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2015)
Article
Plant Sciences
Jarred Yasuhara-Bell, Glorimar Marrero, Asoka De Silva, Anne M. Alvarez
MOLECULAR PLANT PATHOLOGY
(2016)
Article
Plant Sciences
Jarred Yasuhara-Bell, Asoka de Silva, Scott A. Heuchelin, Jennifer L. Chaky, Anne M. Alvarez
Article
Plant Sciences
Jarred Yasuhara-Bell, Glorimar Marrero, Mohammad Arif, Asoka de Silva, Anne M. Alvarez
Article
Plant Sciences
Jarred Yasuhara-Bell, Kerry F. Pedley, Mark Farman, Barbara Valent, James P. Stack
Article
Plant Sciences
C. M. Ayin, R. L. Schlub, J. Yasuhara-Bell, A. M. Alvarez
AUSTRALASIAN PLANT PATHOLOGY
(2015)
Article
Agronomy
Jarred Yasuhara-Bell, Glorimar Marrero, Anne M. Alvarez
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2014)
Article
Plant Sciences
Jarred Yasuhara-Bell, Ryo Kubota, Daniel M. Jenkins, Anne M. Alvarez
News Item
Plant Sciences
J. Yasuhara-Bell, A. S. de Silva, A. M. Alvarez, R. Shimabuku, M. Ko
Article
Plant Sciences
Jarred Yasuhara-Bell, James P. Stack
JOURNAL OF PLANT PATHOLOGY
(2019)
Article
Microbiology
Jarred Yasuhara-Bell, Mohammad Arif, Grethel Y. Busot, Rachel Mann, Brendan Rodoni, James P. Stack
Meeting Abstract
Plant Sciences
G. Marrero, J. Yasuhara-Bell, M. Melzer, A. Alvarez
Proceedings Paper
Plant Sciences
J. Yasuhara-Bell, A. M. Alvarez
IV INTERNATIONAL SYMPOSIUM ON TOMATO DISEASES
(2015)
Meeting Abstract
Plant Sciences
J. H. Yasuhara-Bell, A. M. Alvarez