Comamonas humi sp nova, isolated from soil

A bacterial strain, designated GAU11(T), was isolated from soil in Japan. Cells of the strain were Gram-stain-negative, aerobic, non-motile rods. The 16S rRNA gene sequence of strain GAU11(T) showed high similarity to those of Comamonas zonglianii BF-3(T) (98.8%), Pseudacidovorax intermedius CC21(T) (96.4 %), Acidovorax caeni R-24608(T) (96.2 %), Alicycliphilus denitrificans K601(T) (96.20/o), Pseudorhodoferax soli TBEA3T (95.9 %) and Comamonas terrigena LMG 1253T (95.9%). Strain GAU11(T) contained ubiquinone 8 as the sole ubiquinone and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Its major cellular fatty acids were C-16:0, C-18:1 omega 7c and summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH). The DNA G+C content of strain GAU11(T) was 68.2 mol%. The DNA DNA relatedness between strain GAU11(T) and C. zonglianii DSM 22523(T) was 52 or 68% (reciprocal value). Phenotypic characterization indicated that strain GAU11(T) represents a member of the genus Comamonas, but at the same time distinguished it from C. zonglianii DSM 22523(T). From polyphasic characterization, this strain should be classified as representing a novel species of the genus Comamonas, for which the name Comamonas humi sp. nov. (type strain GAU11(T)=JCM 19903T=DSM 28451(T)) is proposed.