Article
Microbiology
Daniel Maddock, Helene Kile, Sandra Denman, Dawn Arnold, Carrie Brady
Summary: This study investigates the bacterial populations in environmental samples and identifies several strains belonging to the order Enterobacterales. Three novel species of the genus Scandinavium were described, and the descriptions of the genus and the type species were emended.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Chae Yung Woo, Jaisoo Kim
Summary: A novel bacterial strain, designated II4(T), belonging to the genus Lysobacter within the family Xanthomonadaceae, was isolated from soil in Seongnam, Gyeonggi-do, Republic of Korea. The strain was proposed to be named as Lyobacter terrestris sp. nov. based on genomic, chemotaxonomic, phenotypic, and phylogenetic data.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Guo-Hong Liu, Manik Prabhu Narsing Rao, Qian-Qian Chen, Huai Shi, Jian-Mei Che, Bo Liu, Wen-Jun Li
Summary: Two Gram-staining positive strains, FJAT-49825(T) and FJAT-50051(T), isolated from a citrus rhizosphere soil sample, represent two novel species of the genus Neobacillus. These strains have some differences in growth conditions but share high genetic similarity.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Miyoung Won, Hang-Yeon Weon, Seung-Beom Hong, Byeong-Hak Han, Soon-Wo Kwon
Summary: A new species of bacteria, named Arachidicoccus terrestris, was isolated from greenhouse soil in Yeoju-si, Republic of Korea. The strain is Gram-stain-negative, aerobic, non-motile, and rod-shaped or occasionally filamentous-shaped. The bacteria showed high similarity to the genus Arachidicoccus, especially to Arachidicoccus rhizosphaerae. Phenotypic, phylogenetic, and genomic data support the identification of this strain as a novel species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Daniel Maddock, Carrie Brady, Sandra Denman, Dawn Arnold
Summary: Bacterial strains belonging to the family Enterobacteriaceae were isolated from rhizosphere soil and could not be assigned to an existing genus. Through phenotypic testing and genetic analysis, 16 strains were found to form two distinct clusters, leading to the proposal of two new species: Dryocola boscaweniae and Dryocola clanedunesis.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Xianfeng Ge, Shenrong Yang, Cheng Zhen, Wenzheng Liu
Summary: This study isolated two novel species of Actinophytocola and Streptomyces from cotton field soils, and identified them using a polyphasic approach. The strains showed high similarities to Actinophytocola xinjiangensis and Streptomyces iconiensis based on 16S rRNA gene sequences. Phylogenetic and genomic analyses supported the taxonomic delimitation of these two species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Selma Vieira, Katharina J. Huber, Meina Neumann-Schaal, Alicia Geppert, Manja Luckner, Gerhard Wanner, Jorg Overmann
Summary: The two novel species Usitatibacter rugosus sp. nov and Usitatibacter palustris sp. nov, belonging to the new genus Usitatibacter gen. nov, together form the new family Usitatibacteraceae fam. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Carina M. Hall, Anthony L. Baker, Jason W. Sahl, Mark Mayo, Holger C. Scholz, Mirjam Kaestli, James Schupp, Madison Martz, Erik W. Settles, Joseph D. Busch, Lindsay Sidak-Loftis, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Herbert P. Schweizer, Jeffrey M. Warner, Paul Keim, Bart J. Currie, David M. Wagner
Summary: Distinct Burkholderia strains were isolated from soil samples collected in northern Australia, and were identified as two novel species within the Burkholderia pseudomallei complex. These species, named Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov., display unique genomic characteristics and biochemical profiles. The discovery of these new species expands our understanding of this significant bacterial complex.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Minji Bang, Myung Kyum Kim, Hee-Young Jung
Summary: Two novel bacterial strains, BT175(T) and BT728(T), belonging to the genus Hymenobacter, were isolated from soil in the Republic of Korea. They were found to possess distinct molecular and biological characteristics.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Soil Science
Awais Maqsood, Muhammad Shahid, Sabir Hussain, Faisal Mahmood, Farrukh Azeem, Muhammad Tahir, Temoor Ahmed, Muhammad Noman, Irfan Manzoor, Farwa Basit
Summary: The interaction between ACC deaminase producing bacteria and plants can alleviate abiotic stress effects through regulation of ethylene concentration and activation of physiological and genetic mechanisms. In this study, inoculation of rice plants with Burkholderia sp. AQ12 helped improve growth, antioxidative defense system, and expression of tillering-responsive genes under salt stress conditions.
APPLIED SOIL ECOLOGY
(2021)
Article
Environmental Sciences
M. Rasheeda Shameem, J. Mary Isabella Sonali, P. Senthil Kumar, Gayathri Rangasamy, K. Veena Gayathri, V. Parthasarathy
Summary: The study isolated five nitrogen-fixing bacteria from Tea and Groundnut rhizosphere soil, and identified RSKVG 02 as the most optimal strain. The conditions for RSKVG 02 were determined to be pH 7 at 30 degrees C, with 1% glucose and 0.05% ammonium sulfate as carbon and nitrogen sources. This bacterium exhibited plant growth-promoting traits and the potential to enhance soil fertility. Therefore, Rhizobium mayense could be a valuable biofertilizer for crop production, increasing yield, and improving soil fertility.
ENVIRONMENTAL RESEARCH
(2023)
Article
Microbiology
Inhyup Kim, Taegun Seo
Summary: A novel bacterial strain RMG13T was isolated from soil in Siheung-si, Republic of Korea. Through polyphasic analysis, it was determined that RMG13T belongs to the genus Pseudarthrobacter and is most closely related to Pseudarthrobacter sulfonivorans ALLT and Pseudarthrobacter psychrotolerans YJ56T. RMG13T exhibits unique phenotypic, genotypic, and chemotaxonomic characteristics, leading to its proposed classification as Pseudarthrobacter humi sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Daniel Maddock, Dawn Arnold, Sandra Denman, Carrie Brady
Summary: Acute Oak Decline (AOD) is a decline disease affecting native oaks in the UK, with increasing distribution and host range. In this study, the relationship between AOD and Enterobacterales was investigated, leading to the discovery of isolates closely related to the genus Leclercia. These isolates showed potential as plant growth-promoting bacteria and were differentiated from existing species based on genotypic and phenotypic analysis. New species descriptions were proposed.
Article
Microbiology
Onnicha Thayanuwadtanawong, Thitikorn Duangupama, Nantiya Bunbamrung, Pattama Pittayakhajonwut, Chakapong Intaraudom, Sarin Tadtong, Chanwit Suriyachadkun, Ya-Wen He, Somboon Tanasupawat, Chitti Thawai
Summary: A novel actinomycete strain, Streptomyces telluris AA8(T), was isolated from the rhizosphere soil of Mangifera indica in Bangkok, Thailand. The strain showed similarities to Streptomyces roseifaciens MBT76(T) based on the 16S rRNA gene tree, but genome-based analysis revealed differences in nucleotide and amino acid identities. Physiological and biochemical properties further distinguished strain AA8(T) from other Streptomyces species. Additionally, compound 7 (3,4-dihydroxybenzaldehyde) isolated from the strain exhibited potent antioxidant activity comparable to ascorbic acid.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Microbiology
Qinyuan Li, Guiding Li, Lei Lang, Defeng An, Chenglin Jiang, Yi Jiang
Summary: YIM 121974(T) is a novel actinobacterium belonging to the genus Glycomyces, isolated from arid soil in Yunnan Province, China, which can be distinguished from closely related species by a combination of phenotypic and phylogenetic features.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Entomology
Kiran Gurung, Stefanie Nicoline Vink, Joana Falcao Salles, Bregje Wertheim
Summary: The bacterial and fungal communities of the invasive fly D. suzukii and the fruits it infests vary, with fruit types showing different microbial compositions. Bacteria communities of flies remain relatively uniform regardless of fruit source, while fungal communities show a larger resemblance between wild flies and the fruits they develop on. The flies may have formed persistent associations with some bacteria and transmit these across generations.
JOURNAL OF PEST SCIENCE
(2023)
Article
Environmental Sciences
Ademir Sergio Ferreira Araujo, Xiu Jia, Ana Roberta Lima Miranda, Arthur Prudencio de Araujo Pereira, Vania Maria Maciel Melo, Sandra Mara Barbosa Rocha, Romario Martins Costa, Talyta Carine da Silva Saraiva, Lucas William Mendes, Joana Falcao Salles
Summary: This study investigated the impact of different concentrations of composted tannery sludge on bacterial communities. It found that rare bacterial communities were more sensitive to the sludge application, with certain genera increasing in abundance under higher concentrations.
Article
Microbiology
Bram Danneels, Monique Blignaut, Guillaume Marti, Simon Sieber, Peter Vandamme, Marion Meyer, Aurelien Carlier
Summary: The symbiotic relationships between plants of the Rubiaceae and Primulaceae families and Burkholderia bacteria are unique and intimate. This study assembled and analyzed 17 new endophyte genomes, revealing that all endophytes, despite their taxonomic diversity, contained gene clusters linked to the production of specialized metabolites. Acquisition of secondary metabolite gene clusters through horizontal gene transfer is proposed to be a prerequisite for the evolution of a stable association between these endophytes and their hosts.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Amanda Hettiarachchi, Margo Cnockaert, Marie Joossens, David Laureys, Jessika De Clippeleer, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, Dirk C. de Graaf, Peter Vandamme
Summary: This study analyzed the cultivable bacterial communities of different compartments in the invasive insect Vespa velutina, as well as the nest samples, using both cultivation and 16S rRNA amplicon sequencing. The bacterial symbionts in Vespa velutina were dominated by Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas, and Spiroplasma. Lactococcus lactis and Lactiplantibacillus plantarum were generalist core lactic acid bacteria (LAB) symbionts, while Convivina species and Fructobacillus fructosus were highly specialized core LAB symbionts with reduced genome sizes. Sphingomonas and Spiroplasma were non-LAB core symbionts, but they were not isolated. Convivina bacteria were particularly enriched in the hornet crop and included Convivina intestini, a species adapted to amino acid metabolism, and Convivina praedatoris sp. nov., which was adapted to carbohydrate metabolism.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tina Tuerlings, Amanda Hettiarachchi, Marie Joossens, Benoit Geslin, Nicolas J. J. Vereecken, Denis Michez, Guy Smagghe, Peter Vandamme
Summary: The study characterized the gut microbiota of invasive bee Megachile sculpturalis from native and invaded regions. The microbiota communities in bees from invaded regions were similar and different from those in Japan. Core amplicon sequence variants (ASVs) represented micro-organisms commonly present in bee-associated niches. The absence of known bee pathogens and presence of microparasite infections suggest a potential role of pathogen pressure and natural enemies in the invasion success of M. sculpturalis.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Food Science & Technology
Louise Vermote, Jonas De Roos, Margo Cnockaert, Peter Vandamme, Stefan Weckx, Luc De Vuyst
Summary: Belgian lambic beers are produced using traditional craftsmanship, relying on wooden barrels for spontaneous fermentation and maturation. These barrels play a crucial role in establishing a stable microbial ecosystem, minimizing batch-to-batch variations, and preventing flavor deviations. They provide an inoculation source of necessary microorganisms and a microaerobic environment for successful lambic beer production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Juan Guzman, Miyoung Won, Anja Poehlein, Atena Sadat Sombolestani, Daniela Mayorga-Ch, David Laureys, Jessika De Clippeleer, Peter Kaempfer, Rolf Daniel, Andreas Vilcinskas, Peter Vandamme, Soon- Wo Kwon
Summary: In this study, two strains of bacteria were isolated from wasps in Germany and Korea. Molecular and phenotypic analysis showed that these strains are closely related to known species but also have distinct characteristics. Therefore, it is proposed to name these strains Aristophania vespae.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Peter Vandamme, Charlotte Peeters, Amanda Hettiarachchi, Margo Cnockaert, Aurelien Carlier
Summary: Strain LMG 31809 T was isolated from a top soil sample in Belgium and classified as a rare biosphere bacterium that occurs at low abundances in multiple soil and water ecosystems. It represents a new species within a new genus, Govania unica gen. nov., sp. nov, belonging to the class Alphaproteobacteria. The strain is strictly aerobic and uses organic acids and possibly aromatic compounds as growth substrates. Its whole-genome sequence has a size of 3.21 Mbp with a G + C content of 58.99 mol%.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nanzhen Qiao, Julia Bechtner, Margo Cnockaert, Eliza Depoorter, Christian Diaz-Munoz, Peter Vandamme, Luc De Vuyst, Michael G. Ganzle
Summary: This study characterized the genus Periweissella through phylogenetic analysis and genomic analysis, revealing its evolutionary relationship with closely related genera Weissella and Furfurilactobacillus. The study also determined the genetic and physiological traits of the genus. Results showed that Periweissella is the link between rod-shaped heterofermentative lactobacilli and the coccoid Leuconostoc clade, and it is the only heterofermentative genus in the Lactobacillaceae that comprises predominantly motile strains.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Ecology
Amanda Hettiarachchi, Margo Cnockaert, Marie Joossens, Antoine Gekiere, Ivan Meeus, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, Peter Vandamme
Summary: We characterized the microbial communities in different parts of four wild solitary bee species and found that the dominant bacteria were endosymbionts such as Wolbachia and Spiroplasma. Other bacterial and yeast genera were associated with environmental sources. The bacterial communities varied between bee species, gut compartments, and ovaries, suggesting a selective process influenced by floral and host traits. Many of the gut symbionts identified in this study exhibited metabolic versatility.
Article
Biotechnology & Applied Microbiology
Atena Sadat Sombolestani, Dries Bongaerts, Eliza Depoorter, Ilse Cleenwerck, Anneleen D. Wieme, Scott J. Britton, Stefan Weckx, Luc De Vuyst, Peter Vandamme
Summary: Polyphasic taxonomic and comparative genomic analyses revealed a novel species among acetic acid bacteria, named Brytella acorum gen. nov., sp. nov., represented by lambic beer isolates including strain LMG 32668T and the kombucha isolate LMG 32879. This species has Acidomonas methanolica as its closest phylogenomic neighbor and possesses metabolic characteristics typical of acetic acid bacteria. The absence of glycolysis pathway and the presence of both aerobic respiration and oxidative fermentation for energy metabolism are notable features. Antibiotic resistance genes and virulence factors are absent.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Biology
Luc Cornet, Benoit Durieu, Frederik Baert, Elizabet D'hooge, David Colignon, Loic Meunier, Valerian Lupo, Ilse Cleenwerck, Heide-Marie Daniel, Leen Rigouts, Damien Sirjacobs, Stephane Declerck, Peter Vandamme, Annick Wilmotte, Denis Baurain, Pierre Becker
Summary: A bioinformatics toolbox called GEN-ERA has been developed by the Belgian Coordinated Collections of Microorganisms. It allows researchers to perform robust phylogenomic analyses on bacteria and small eukaryotes without specific training in bioinformatics. The toolbox provides workflows for genome downloading, quality assessment, contamination estimation, tree reconstruction, average nucleotide identity comparisons, and metabolic modeling. It has been tested on various microorganisms and used in a case study on Gloeobacterales for microbial taxonomy.
Article
Microbiology
Juliana Botero, Atena Sadat Sombolestani, Margo Cnockaert, Charlotte Peeters, Wim Borremans, Luc De Vuyst, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, German Bonilla-Rosso, Philipp Engel, Peter Vandamme
Summary: This study provides insights into the diversity and function of Commensalibacter bacteria in various insect species. Through analysis of their whole-genome sequences, it was found that Commensalibacter strains can be classified into four species, which share similar genetic pathways for central metabolism. However, there are differences in genome size, G+C content, amino acid metabolism, and carbohydrate-utilizing enzymes among these species. Notably, the study on the Western honey bee symbiont, C. melissae, revealed its unique characteristics such as reduced genome size and a large number of species-specific gene clusters.
Article
Environmental Sciences
Leon Marshall, Nicolas Leclercq, Timothy Weekers, Insafe El Abdouni, Luisa G. Carvalheiro, Michael Kuhlmann, Denis Michez, Pierre Rasmont, Stuart P. M. Roberts, Guy Smagghe, Peter Vandamme, Thomas Wood, Nicolas J. Vereecken
Summary: Visitation by wild bee species is essential for apple production, but they are vulnerable to climate change. We modeled the distribution range shifts of key apple-pollinating bee species under different climate change scenarios and found that overall contractions in range are limited. However, under the worst-case scenario, some countries such as France and Italy may experience significant loss in bee populations and potential pollination services. Climate change is not the only threat to apple pollination, and future deficits will also depend on orchard management and ecological infrastructure.
GLOBAL ENVIRONMENTAL CHANGE-HUMAN AND POLICY DIMENSIONS
(2023)
Article
Microbiology
H. Pieter J. van Veelen, Joana Falcao Salles, Kevin D. Matson, G. Sander van Doorn, Marco van der Velde, B. Irene Tieleman
Summary: The microbial environment of birds can shape maternal effects on egg immune function. Maternal regulation of yolk IgY levels based on cues from the parental microbial environment may prove important for offspring to thrive in the anticipated microbial environment.
