Roseomonas soli sp nova, isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris)

A bacterial strain, designated 5N26(T), was isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Cells of this strain were Gram-reaction-negative, strictly aerobic, motile, non-spore-forming rods, and catalase- and urease-negative. The major fatty acids of strain 5N26(T) were C-16:0 (7.5%), C-18:1 2-OH (13.4%) and summed feature 8 (C-18:1 omega 6c and/or C-18:1 omega 7c; 63.2%). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The G+C content of the genomic DNA of strain 5N26(T) was 68.3 mol%. 16S rRNA gene sequence analysis showed that strain 5N26(T) was phylogenetically related to Roseomonas lacus TH-G33(T) and Roseomonas terrae DS-48(T) (97.0 % and 96.6% sequence similarity, respectively). The results of genotypic and phenotypic data showed that strain 5N26(T) could be distinguished from phylogenetically related species, and that this strain represented a novel species within the genus Roseomonas, for which the name Roseomonas soli sp. nov. (type strain 5N26(T)=KACC 16376(T)=NBRC 109097(T)) is proposed.