Flaviramulus ichthyoenteri sp nov., an N-acylhomoserine lactone-degrading bacterium isolated from the intestine of a flounder (Paralichthys olivaceus), and emended descriptions of the genus Flaviramulus and Flaviramulus basaltis

A Gram-stain-negative, strictly aerobic, yellow-pigmented, rod-shaped and N-acylhomoserine lactone-degrading bacterium, designated strain Th78(T), was isolated from the intestine of a cultured flounder (Paralichthys olivaceus). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Th78(T) belonged to the genus Flaviramulus (family Flavobacteriaceae) and showed the highest 16S rRNA gene sequence similarity to Flaviramulus basaltis H35(T) (96.70%). Optimal growth occurred in the presence of 2-3% (w/v) NaCl, at pH 7.0-8.0 and at 28 degrees C. The major fatty acids were iso-C-15:0 3-OH, iso-C-15:1 G and iso-C-17:0 3-OH. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and three unidentified polar lipids. Menaquinone 6 (MK-6) was the only respiratory quinone. The DNA G+C content of strain Th78(T) was 31.5 mol%. On the basis of polyphasic analysis, strain Th78(T) is considered to represent a novel species of the genus Flaviramulus, for which the name Flaviramulus ichthyoenteri sp. nov. is proposed. The type strain is Th78(T) (=JCM 18634(T)=KCTC 32142(T)=DSM 26285(T)). Emended descriptions of the genus Flaviramulus and Flaviramulus basaltis are also proposed.