Paenibacillus catalpae sp nov., isolated from the rhizosphere soil of Catalpa speciosa

A bacterial strain, designated D75(T), was isolated from the rhizosphere soil of Catalpa speciosa. Phylogenetic analysis based on the complete 16S rRNA gene sequence revealed that strain D75(T) was a member of the genus Paenibacillus. High levels of 16S rRNA gene sequence similarity were found between strain D75(T) and Paenibacillus glycanilyticus DS-1(T)(99.2%), Paenibacillus xinjiangensis B538(T) (97.5%) and Paenibacillus castaneae Ch-32(T) (97.2%). The chemotaxonomic properties of strain D75(T) were consistent with those of the genus Paenibacillus: the cell-wall peptidoglycan type was based on meso-diaminopimelic acid (A1 gamma), the predominant menaquinone was MK-7, and the major fatty acids were anteiso-C-15:0, iso-C-16:0 and C-16:0. However, levels of DNA DNA relatedness between strain D75(T) and P. glycanilyticus NBRC 16618(T), P xinjiangensis DSM 16970(T) and P. castaneae DSM 19417(T) were 35, 20 and 18%, respectively. On the basis of phenotypic and chemotaxonomic analyses, phylogenetic data and DNA DNA relatedness values, strain D75(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus catalpae sp. nov. is proposed. The type strain is D75(T) (=DSM 24714(T)=CGMCC 1.10784(T)).