Article
Biotechnology & Applied Microbiology
Pavel Svec, Hans-Juergen Busse, Karel Sedlar, Jana Musilova, Stanislava Kralova, Eva Stankova, Ondrej Sedo, Daniel Krsek, Vendula Koublova, Lucie Kristofova, Ivo Sedlacek
Summary: A taxonomic study of bacterial strains isolated from wild Adelie penguins in Antarctica identified four new species in the genus Corynebacterium.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Manon Boxberger, Sibylle Magnien, Angeline Antezack, Clara Rolland, Marine Makoa Meng, Cheikh Ibrahima Lo, Bernard La Scola, Nadim Cassir
Summary: By studying the human skin microbiota, we isolated a new bacterium called Leucobacter manosquensis sp. nov. Marseille-Q4368 strain from the forehead of a healthy woman. This bacterium showed phenotypical and genomic differences from its closely related species.
Article
Microbiology
Lena Schaffert, Matthias Ruwe, Johanna Milse, Katharina Hanuschka, Vera Ortseifen, Julian Droste, David Brandt, Laura Schluter, Yvonne Kutter, Svenja Vinke, Prisca Viehofer, Lucas Jacob, Nils-Christian Lubke, Eva Schulte-Berndt, Carsten Hain, Marten Linder, Pascal Schmidt, Lars Wollenschlager, Tobias Luttermann, Eric Thieme, Julia Hassa, Markus Haak, Manuel Wittchen, Almut Mentz, Marcus Persicke, Tobias Busche, Christian Ruckert
Summary: Three novel corynebacterial species were isolated from soil in Germany, characterized by their coccoid or irregular rod-shaped morphology and unique genetic structure. They were named Corynebacterium kalinowskii sp. nov., Corynebacterium comes sp. nov., and Corynebacterium occultum sp. nov. based on their phenotypic and genotypic characteristics.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Gui Zhang, Jing Yang, Xin-He Lai, Dong Jin, Shan Lu, Zhihong Ren, Tian Qin, Ji Pu, Yajun Ge, Yanpeng Cheng, Caixin Yang, Xianglian Lv, Yifan Jiao, Ying Huang, Jianguo Xu
Summary: Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria isolated from animal tissues and human faeces were identified as belonging to the genus Corynebacterium. They were named Corynebacterium zhongnanshanii, Corynebacterium lujinxingii, and Corynebacterium wankanglinii based on phylogenetic analyses. These novel species showed distinctive characteristics in terms of DNA content, relatedness with other species, fatty acids composition, and optimal growth conditions.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Juan Zhou, Mingchao Xu, Wentao Guo, Jing Yang, Ji Pu, Xin-He Lai, Dong Jin, Shan Lu, Sihui Zhang, Yuyuan Huang, Wentao Zhu, Ying Huang, Han Zheng, Jianguo Xu
Summary: Four new Gram-stain-positive, non-motile and asporous bacilli from the Qinghai-Tibet Plateau were taxonomically characterized as two novel species of the genus Corynebacterium based on 16S rRNA and rpoB gene sequence analyses. The two novel type strains shared high similarities in gene sequences but were differentiated by TAP-PCR DNA fingerprint and MALDI-TOF MS spectrum patterns.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sonja Kittl, Eveline Studer, Isabelle Brodard, Andreas Thomann, Jorg Jores
Summary: Several strains of an undescribed Corynebacterium species were isolated from bovine mastitic milk samples. Genotypic and phenotypic analysis confirmed the identification of a new species named Corynebacterium uberis.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Environmental Sciences
Ya-Ping Sun, Bei-Bei Wang, Zhang-Ping Wu, Xi-Wen Zheng, Jing Hou, Heng-Lin Cui
Summary: Five novel halophilic archaeal strains were isolated from coastal saline sediments and sea salt in China. They demonstrated typical morphology and growth characteristics and showed high similarities with existing species. However, based on genetic and phylogenetic analyses, it is confirmed that these strains represent five new species. This is the first report of a novel haloarchaeon isolated from a marine intertidal zone.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Microbiology
Jihane Kabtani, Muriel Militello, Stephane Ranque
Summary: The genus Coniochaeta belongs to the class Ascomycota and the family Coniochaetaceae. Some species are plant and animal pathogens, while others are involved in human diseases. A new species, Coniochaeta massiliensis, was described and characterised, with genetic and phenotypic analysis showing its uniqueness compared to other species in the genus.
Article
Multidisciplinary Sciences
Afaf Hamame, Reham Magdy Wasfy, Cheikh Ibrahima Lo, Florence Fenollar, Didier Raoult, Pierre-Edouard Fournier, Linda Houhamdi
Summary: Two new bacterial strains, Marseille-P2698(T) and Marseille-P2260(T), were isolated from human stools using the culturomic method. These two strains were fully described using the taxonogenomic approach. Marseille-P2698(T) was identified as a Gram-negative, motile, non-spore-forming, rod-shaped bacterium, while Marseille-P2260(T) was identified as a Gram-positive, motile, spore-forming rod-shaped bacterium.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Qiang Luo, Qianming Chen, Junhui Feng, Tianqi Zhang, Li Luo, Cha Chen, Xiaoyan Liu, Ning Xu, Pinghua Qu
Summary: By characterizing and analyzing the isolates, researchers identified two novel species that were closely related to but hard to distinguish from C. kroppenstedtii. These isolates were mainly obtained from breast specimens and may play a role in the pathogenesis of mastitis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Genetics & Heredity
Hairui Wang, Yixin Gu, Guilan Zhou, Xiaoli Chen, Xin Zhang, Zhujun Shao, Maojun Zhang
Summary: In 2019, two different bacterial isolates were obtained from the fecal samples of separate dogs in Beijing, China. These isolates exhibit unique morphological and genetic characteristics, suggesting that they represent a novel species within the genus Helicobacter.
FRONTIERS IN GENETICS
(2023)
Article
Multidisciplinary Sciences
Yuanqiang Zou, Xiaoqian Lin, Wenbin Xue, Li Tuo, Ming-Sheng Chen, Xiao-Hui Chen, Cheng-hang Sun, Feina Li, Shao-wei Liu, Ying Dai, Karsten Kristiansen, Liang Xiao
Summary: Two novel anaerobic strains, designated AF52-21(T) and CM04-06(T), were isolated from faeces of healthy Chinese donors using a pure culture strategy. Phylogenetic analysis revealed that these strains clustered with Faecalibacterium species, supporting their recognition as two new species within the genus. Genomic analysis showed differences below the proposed species threshold, confirming their uniqueness.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Aurelie Morand, Mamadou Lamine Tall, Edmond Kuete Yimagou, Issa Isaac Ngom, Cheikh Ibrahima Lo, Florent Cornu, Michel Tsimaratos, Jean-Christophe Lagier, Anthony Levasseur, Didier Raoult, Pierre-Edouard Fournier
Summary: This study describes a new species Anaerococcus urinomassiliensis, isolated from the urine of a 17-year-old boy with autoimmune hepatitis and membranoproliferative glomerulonephritis. The strain exhibits specific genetic characteristics, is primarily of human origin, and belongs to the genus of anaerobic bacteria.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Jung-Yun Lee, Dong-Hun Lee, Dong-Hun Kim
Summary: Gram-stain-negative bacterial strains NC18(T) and NC20, isolated from sediment near-vertical borehole effluent originating 714 m below the subsurface in Minnesota, USA, belong to the genus Martelella and are proposed as a novel species, Martelella soudanensis sp. nov.
Article
Microbiology
Ping-Hua Qu, Hai-Min Luo, Jun-Hui Feng, Song Li, Cha Chen, Lei Dong, Yu-Zhen Ming, Wen-Jun Li, Ying Lin
Summary: Strain SZY PN-1(T) is a novel Gram-negative, yellow-pigmented bacterium isolated from a skin sample of a healthy Chinese male. It shares high similarities with two invalid-published species, forming a separate branch within the family Sphingosinicellaceae based on phylogenetic analysis. A new genus and species, Sandaracinobacteroides hominis gen. nov., sp. nov., is proposed based on its phenotypic and genotypic features.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Ecology
Stefanie P. Glaeser, Liliana M. R. Silva, Rui Prieto, Monica A. Silva, Angel Franco, Peter Kampfer, Carlos Hermosilla, Anja Taubert, Tobias Eisenberg
Summary: The intestinal microbiomes of large baleen whales and toothed whales show significant differences at the family and genus levels, while similarities were observed at the phylum level. The most abundant phyla found were Firmicutes, Bacteroidetes, Proteobacteria, Tenericutes, and Spirochaeta.
Review
Microbiology
Marta Prygiel, Ewa Mosiej, Paulina Gorska, Aleksandra A. Zasada
Summary: The DTP vaccine prevents diphtheria, tetanus, and pertussis, initially as monovalent vaccines and later evolving into multiple effective vaccines. Efforts to combine DTP with other routine vaccines for infants were quickly stimulated as development progressed.
FUTURE MICROBIOLOGY
(2022)
Article
Food Science & Technology
Kostas Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Michaela Hempen, Lieve Herman
Summary: The qualified presumption of safety (QPS) approach is a generic pre-evaluation method for the safety of biological agents. It assesses published data for each agent based on taxonomic identity, relevant knowledge, and safety concerns. The QPS list has been updated to reflect the revised taxonomy, synonyms, and specific safety concerns.
Article
Food Science & Technology
Soren Saxmose Nielsen, Dominique Joseph Bicout, Paolo Calistri, Elisabetta Canali, Julian Ashley Drewe, Bruno Garin-Bastuji, Jose Luis Gonzales Rojas, Christian Gortazar Schmidt, Mette Herskin, Virginie Michel, Miguel Angel Miranda Chueca, Barbara Padalino, Paolo Pasquali, Helen Clare Roberts, Hans Spoolder, Karl Stahl, Antonio Velarde, Arvo Viltrop, Christoph Winckler, Jeroen Dewulf, Luca Guardabassi, Friederike Hilbert, Rodolphe Mader, Jesus L. Romalde, Peter Smith, Francesca Baldinelli, Lisa Kohnle, Julio Alvarez
Summary: This article evaluates antimicrobial-resistant bacteria that pose a threat to the health of kept fish species and provides an overview of the global status of resistance in certain bacteria. Based on limited scientific evidence, no specific bacteria were identified as the most relevant antimicrobial-resistant species in the EU's kept fish species.
Article
Microbiology
Yina Cifuentes, Andreas Vilcinskas, Peter Kampfer, Stefanie P. Glaeser
Summary: Hermetia illucens larvae, also known as black soldier fly larvae (BSFL), are efficient in converting organic waste into high-quality biomass. This study explored the role of gut microbes in this process by culturing members of the core gut microbiota. Two cultivation strategies were employed, dilution-to-extinction cultivation and direct plating, resulting in the isolation of a diverse range of bacterial strains. Phylogenetic analysis identified predominant genera, such as Providencia, Proteus, Morganella, Enterococcus, Bacillus, and members of the family Enterobacteriaceae, which have been previously associated with the BSFL gut microbiota. Genomic fingerprinting provided insights into the genetic diversity of the cultured bacteria that could not be obtained solely through 16S rRNA gene sequencing.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Peter Kaempfer, Stefanie P. Glaeser, Hans-Juergen Busse, John A. McInroy, Dominique Clermont, Alexis Criscuolo
Summary: A novel bacterium belonging to the genus Pseudoneobacillus was isolated from the rhizosphere of maize roots, showing similarities to Neobacillus species but with distinct differences. Therefore, we propose the name Pseudoneobacillus rhizosphaerae gen. nov., sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernandez Escamez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Sandra Correia, Lieve Herman
Summary: The qualified presumption of safety (QPS) approach is a regularly updated generic pre-evaluation of the safety of microorganisms in the food or feed chains. It is used to support the work of EFSA's Scientific Panels. In this study, 52 microorganisms were evaluated between April and September 2022.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Sara Bover-Cid, Marianne Chemaly, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Bojan Blagojevic, Inge Van Damme, Michaela Hempen, Winy Messens, Declan Bolton
Summary: This study examines the impact of dry-ageing of beef and wet-ageing of beef, pork, and lamb on microbiological hazards and spoilage bacteria, and describes current practices. It differentiates between 'standard fresh' and wet-aged meat based on duration. The study collates data on key parameters (time, temperature, pH, and a(w)) using a literature survey and questionnaires. Various microbiological hazards and spoilage bacteria are identified in aged meats, and methods to prevent their growth are discussed. The study concludes that ageing meat under defined and controlled conditions can achieve similar or lower levels of microbiological hazards and spoilage bacteria than standard fresh meat preparation.
Article
Biochemistry & Molecular Biology
Tomasz Wolkowicz, Katarzyna Zacharczuk, Aleksandra Anna Zasada
Summary: Infections caused by non-toxigenic Corynebacterium diphtheriae have been increasing in Poland, with the most common strains being ST8 biovar gravis. This study analyzed 30 strains isolated between 2017 and 2022, as well as six previously isolated strains, using classic methods and whole genome sequencing. The appearance of new strains in 2022 and the isolation of the first non-toxigenic tox gene-bearing strain suggest that C. diphtheriae should be considered a pathogen of special public health concern in Poland.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Waldemar Rastawicki, Rafal Gierczynski, Aleksandra Anna Zasada
Summary: This study investigated the dynamics of antibody avidity and IgG subclasses in individuals vaccinated with the BNT162B2 mRNA vaccine and in COVID-19 patients. The results showed that IgG1 was the predominant subclass in both groups. IgG4 levels and IgG avidity significantly increased 7 months after the first two doses of the vaccine and after the third dose. IgG2 and IgG3 levels were generally low.
Article
Infectious Diseases
Catia Pacifico, Marc M. S. M. Woesten, Friederike Hilbert
Summary: Campylobacter (C.) spp. are significant foodborne, bacterial, and zoonotic pathogens globally. It is crucial to monitor the resistance of foodborne bacterial pathogens as a part of the One Health approach to control antimicrobial resistance. The discovery and functionality of new resistance genes are important for improving screening methods using whole genome sequencing (WGS). The tet(O)_3 gene, similar to tet(O) in Streptococcus pneumoniae, has been identified in C. jejuni isolates, conferring tetracycline resistance below the epidemiological cut-off value. These isolates also possess other resistance genes, such as fluoroquinolone resistance and a multidrug efflux pump.
Article
Veterinary Sciences
Elfriede Wilhelm, Irina Korschineck, Michael Sigmund, Peter Paulsen, Friederike Hilbert, Wigbert Rossmanith
Summary: American foulbrood is a deadly disease specific to honey bees caused by the spore-forming bacterium Paenibacillus larvae. Detecting the disease early is crucial in controlling its impact. A voluntary monitoring program in Lower Austria utilized traditional pathogen detection and DNA-based detection to identify infected colonies without the need for spore germination. The results showed comparable efficacy to culture-based methods with a significant time advantage.
VETERINARY SCIENCES
(2023)
Review
Virology
Aleksandra Anna Zasada, Aniela Darlinska, Aldona Wiatrzyk, Katarzyna Woznica, Kamila Forminska, Urszula Czajka, Malgorzata Glowka, Klaudia Lis, Paulina Gorska
Summary: The development of COVID-19 vaccines has been unprecedented in terms of speed, with various vaccine platforms receiving approval for human application.
Article
Biochemistry & Molecular Biology
Marta Prygiel, Ewa Mosiej, Karol Wdowiak, Daniel Rabczenko, Aleksandra Anna Zasada
Summary: There is a growing interest in developing new-generation purified antigen vaccines with higher safety profiles. Subunit vaccines have lower immunogenicity and induce weaker and shorter-lasting immune responses compared to whole-cell vaccines.
Article
Immunology
Paulina Gorska, Malgorzata Glowka, Katarzyna Woznica, Aleksandra A. Zasada
Summary: This study developed a modified shake test for prequalifying potentially frozen vaccines. The results showed significant differences in the precipitation time between frozen and non-frozen vaccines.
CLINICAL AND EXPERIMENTAL VACCINE RESEARCH
(2022)
