Shewanella indica sp nov., isolated from sediment of the Arabian Sea

A Gram-negative, facultatively anaerobic, rod-shaped, catalase- and oxidase-positive bacterium, motile by means of a single polar flagellum and designated strain KJW27(T), was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was beta-haemolytic and grew with 0-100/s (w/v) NaCl, at 10-45 degrees C and at pH 6.5-10, with optimum growth with 2% (w/v) NaCl, at 37 degrees C and at pH 7.5. The major fatty acids were iso-C-15 : 0 (22.2%), C-17 : 1 omega 8C (21%), summed feature 3 (comprising C-16 : 1 omega 7C and/or C-16 : 1 omega 6c; 10.2%), C-16 : 0 (7.1 %), iSO-C-13 : 0 (5.6%) and C-17 : 0 (4.4 %). The DNA G + C content was 51.2 mol%. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences showed that strain KJW27(T) forms a lineage within the genus Shewanella and is closely related to Shewanella algae ATCC 51192(T) (98.8 %), Shewanella haliotis DWO1(T) (98.8%) and Shewanella chilikensis JC5(T) (98.2%). Sequence identity with other members of this genus ranges from 92.2 to 96.4%. The DNA DNA relatedness of strain KJW27(T) with S. algae ATCC 51192(T), S. haliotis DWO1(T) and S. chilikensis JC5(T) was 52, 44 and 33%, respectively. The phenotypic, genotypic and DNA DNA relatedness data indicate that strain KJW27(T) should be distinguished from S. algae ATCC 51192(T), S. haliotis DWO1(T) and S. chilikensis JC5(T). On the basis of the data presented in this study, strain KJW27(T) represents a novel species, for which the name Shewanella indica sp. nov. is proposed. The type strain is KJW27(T) (=KCTC 23171(T) =BCC 41031(T) = NCIM 5388(T)).