Brevibacterium salitolerans sp nov., an actinobacterium isolated from salt-lake sediment

A novel bacterium, designated TRM 415(T), belonging to the genus Brevibacterium, was isolated from a sediment sample from a salt lake in Xinjiang province, China. Comparative 16S rRNA gene sequence analysis indicated that strain TRM 415(T) was phylogenetically most closely related to Brevibacterium album YIM 90718(T) (98.4 % sequence similarity) and had low similarity (<95.5%) to other species of the genus Brevibacterium; however, DNA-DNA hybridization studies between strain TRM 415(T) and B. album YIM 90718(T) showed only 41.3% relatedness. Strain TRM 415(T) possessed meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, MK-8(H-2) as the major menaquinone and polar lipids including phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were anteiso-C-17:0 and anteiso-C-15:0 The genomic DNA G + C content was 69 mol%. Based on the evidence from this polyphasic study, strain TRM 415(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium salitolerans sp. nov. is proposed. The type strain is TRM 415(T) (=JCM 15900(T) =CCTCC AB 208328(T) =KCTC 19616(T)).