Enterobacter arachidis sp nov., a plant-growth-promoting diazotrophic bacterium isolated from rhizosphere soil of groundnut

A methylotrophic nitrogen-fixing bacterial strain, Ah-143(T), isolated from the rhizosphere soil of field-grown groundnut was analysed by a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated strain Ah-143(T) to the family Enterobacteriaceae, with Enterobacter radicincitans and Enterobacter cowanii as the closest relatives. The strain is Gram-stain-negative, non-spore-forming, aerobic and motile, having straight rod-shaped cells with a DNA G+C content of approximately 53.2 mol%. The strain utilizes methanol as a carbon source and the mxaF gene was closely related to the mxaF gene of members of the genus Methylobacterium. The fatty acid profile consisted of C(16:0), C(17:0) cyclo, C(18:1)omega 7c, summed feature 2 (iso-C(16:1) I and/or C(14:0) 3-OH) and summed feature 3 (iso-C(15:0) 2-OH and/or C(16:1)omega 7c) as the major components. DNA DNA relatedness of strain Ah-143(T) with its close relatives was less than 20%. On the basis of the phylogenetic analyses, DNA DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the strain represents a novel species of the genus Enterobacter and should be named Enterobacter arachidis sp. nov. The type strain is Ah-143(T) (=NCIMB 14469(T) =KCTC 22375(T)).