Article
Microbiology
Jiyoun Kim, Geeta Chhetri, Inhyup Kim, Yoonseop So, Taegun Seo
Summary: Three novel bacterial strains belonging to the genus Paenibacillus were isolated from rhizosphere soils and characterized. Phylogenetic analysis showed that they formed three distinct branches and represented novel genospecies. The strains were proposed as new species and given specific names.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Shan Shan Qi, Margo Cnockaert, Aurelien Carlier, Peter A. Vandamme
Summary: This study isolated six novel species within the genus Paenibacillus from the phyllosphere of Arabidopsis thaliana plants, highlighting their unexplored biotechnological potential. These bacteria were identified through MALDI-TOF mass spectra and comparative 16S rRNA gene sequencing, with distinct biochemical characteristics identified for each new species. The proposed names and type strains for each novel species were also provided.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Nar Singh Chauhan, Neetha Joseph, Shraddha Shaligram, Nikita Chavan, Amaraja Joshi, Dhiraj Dhotre, Tushar Lodha, Yogesh Shouche
Summary: A novel rod-shaped bacterial strain SM69(T) was isolated from soil samples in Rohtak, Haryana, India. It belongs to the genus Paenibacillus and has been proposed as a new species named Paenibacillus oleatilyticus sp. nov.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Kyung Hyun KIm, Ye Lin Seo, Ju Hye Baek, Hyun Mi Jin, Che Ok Jeon
Summary: The study describes the isolation of a strict aerobic bacterium, Paenibacillus agri sp. nov., from soil in Korea, with unique fatty acids and polar lipids compositions. The bacterium exhibited a distinct phyletic lineage based on phylogenetic analysis of 16S rRNA gene sequences, showing high resistance and unique characteristics.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Hyosun Lee, Dhiraj Kumar Chaudhary, Oung Bin Lim, Dong-Uk Kim
Summary: A novel bacterial strain S3N08(T) was isolated from agricultural soil, which showed white color, rod-shaped morphology and motility. It belonged to the genus Paenibacillus and had the closest relative of Paenibacillus periandrae PM10(T) with 95.6% 16S rRNA gene sequence similarity.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Microbiology
Peter Kaempfer, Andre Lipski, John A. McInroy, Dominique Clermont, Lucie Lamothe, Stefanie P. Glaeser, Alexis Criscuolo
Summary: Two Gram-stain-positive, aerobic, endospore-forming bacterial strains (JJ-7T and JJ-60T) isolated from the rhizosphere of Zea mays were classified as members of the genus Paenibacillus based on 16S rRNA gene sequence similarity comparisons. Both strains represented novel species (Paenibacillus auburnensis sp. nov. and Paenibacillus pseudetheri sp. nov.) within the genus Paenibacillus. Genomic comparisons and polar lipid profiles supported the taxonomic allocation, and physiological and biochemical characteristics further differentiated the strains from closely related species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Hongxiang Liu, Lijing Lu, Sijin Wang, Meng Yu, Xiaoyun Cao, Sufang Tang, Haijiao Bai, Shihong Ma, Ruina Liu, Rui Liu, Xiaoying Jiang, Su Yao, Jianqiang Shao
Summary: TB2019(T) is a Gram-stain-positive, facultatively anaerobic bacterium, closely related to other species of the genus Paenibacillus. It shows good growth capability within a range of temperature, pH, and NaCl concentration.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Yu-Feng Chen, Lin Ye, Hui-Qin Huang, Ming-Guo Jiang, Yong-Hua Hu, Dong-Mei Sun, Kun-Lian Mo
Summary: A new bacterial strain named Paenibacillus oceani was isolated from the South China Sea, showing unique characteristics in terms of cell wall structure and major lipid components.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Jungeun Kim, Hye Su Jung, Ju Hye Baek, Byung Hee Chun, Shehzad Abid Khan, Che Ok Jeon
Summary: Bacterial strain 5J-6(T) was isolated from soil in Cheongnyeongpo, Republic of Korea, and belonged to the genus Paenibacillus. Through 16S rRNA gene sequence analysis, 5J-6(T) was identified as a novel species and named Paenibacillus silvestris sp. nov.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Vipool Thorat, Kiran Kirdat, Bhavesh Tiwarekar, Priyanka Dhanavade, Prachi Karodi, Yogesh Shouche, Shivaji Sathe, Tushar Lodha, Amit Yadav
Summary: Two novel strains, UniB2(T) and UniB3(T), were isolated from digestive syrup and identified as new species of Paenibacillus and Niallia, respectively. These strains differed from their phylogenetically related neighbors in terms of fatty acid composition, DNA G+C content, and carbon utilization pattern.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Joo Hyun Han, Sang Eun Lee, Sathiyaraj Srinivasan
Summary: Strain 18JY21-1(T) is a novel species of Paenibacillus, named Paenibacillus albiflavus sp. nov., based on phylogenetic, chemotaxonomic, and phenotypic data analysis.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Lei Sun, Panpan Wang, Dongfang Zhao, Jiashuo Wang, Qiji Wu, Lin Liu, Zhitang Lyu
Summary: Strain R196(T) is a novel species of the genus Paenibacillus, named Paenibacillus cymbidii sp. nov., with distinctive cellular polar lipids and respiratory quinone composition. The genome of R196T contains 7617 predicted protein-coding genes, with a DNA G+C content of 57.7 mol%.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
You Ju Ham, Ji Won Jeong, Dong Hyeon Lee, Seung Bum Kim
Summary: Two novel species of Paenibacillus were isolated from plant roots, showing potential plant-growth-promoting characteristics. They were named Paenibacillus artemisiicola sp. nov. and Paenibacillus lignilyticus sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Lei Yan, Jiajia Shi, Jiahui Cao, Meng Zhang, Linxia Chen, Lixin Wang, Zhihua Bao
Summary: A newly isolated bacterium, strain WST5T, was identified as a Gram-stain-positive, motile, rod-shaped, and facultatively anaerobic bacterium. Phylogenetic analysis revealed its close relationship with Paenibacillus aestuarii CJ25T, with a similarity of 96.8%. The strain's genome size was 6.5 Mb, containing predicted protein-coding genes and having a G+C content of 46.6%. Based on multiple analyses, strain WST5T is proposed as a novel species of the genus Paenibacillus, named Paenibacillus sedimentum sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Rong Tang, Qi Zhang, Yin Ye, Shang Yang, Tao Fu, Guo-Hong Liu, Shun-Gui Zhou
Summary: Two thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost in Shanxi Province, PR China. They belong to a novel species of the genus Brevibacillus, named Brevibacillus composti, based on physiological properties, chemotaxonomic characteristics, and genetic analysis. These strains show high sequence similarity and phylogenetic relationship with Brevibacillus borstelensis.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Ines Leao, Teresa Bento de Carvalho, Valentina Henriques, Catarina Ferreira, Benedita Sampaio-Maia, Celia M. Manaia
Summary: Pseudomonadota is less prevalent in the human oral cavity despite being one of the most represented phyla in the environment. This review provides an overview of the diversity and ecology of Pseudomonadota in the oral cavity, which has been largely underexplored in scientific literature. The screening of scientific literature and human microbiome databases revealed more than 1300 reports of Pseudomonadota in the oral cavity, mainly belonging to Beta- and Gammaproteobacteria classes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Joao P. F. Venancio, Sara Ribeirinho-Soares, Luisa C. Lopes, Luis M. Madeira, Olga C. Nunes, Carmen S. D. Rodrigues
Summary: In this study, a combination of coagulation/flocculation and Fenton processes was used for tertiary treatment of urban wastewater. The results showed that the best removals of turbidity and enterobacteria were achieved with a coagulant dosage of 120 mg/L and the natural pH of the effluent. The treated water met the strictest limit for E. coli, but had a high relative abundance of potentially hazardous bacteria, emphasizing the need for microbiological characterization before reuse.
ENVIRONMENTAL RESEARCH
(2023)
Review
Environmental Sciences
Celia M. Manaia, Diana S. S. Aga, Eddie Cytryn, William H. H. Gaze, David W. W. Graham, Jianhua Guo, Anne F. C. Leonard, Liguan Li, Aimee K. K. Murray, Olga C. C. Nunes, Sara Rodriguez-Mozaz, Edward Topp, Tong Zhang
Summary: Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are prevalent environmental contaminants. Factors driving the evolution and dissemination of antibiotic resistance are not well understood. ARB and ARGs are often found in areas impacted by human activity, particularly in environments affected by fecal wastes or mixed chemical contaminants. Our review explores the relationship between chemical contaminants and ARB/ARGs, including co-occurrence, potential biostimulation, selective pressure effects, and mitigation strategies. We also discuss evidence and strategies for assessing human health risks associated with environmental antibiotic resistance.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jovana Kabic, Gianuario Fortunato, Ivone Vaz-Moreira, Dusan Kekic, Milos Jovicevic, Jovan Pesovic, Lazar Ranin, Natasa Opavski, Celia M. Manaia, Ina Gajic
Summary: This nationwide study aimed to investigate the molecular characteristics, resistance mechanisms, genetic context, and clonal relationship of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa in Serbia. The study found that 320 out of 5334 isolates collected from 2018 to 2021 were identified as P. aeruginosa, and 43.1% of carbapenem-resistant isolates carried the bla(NDM-1) gene. The study also revealed the presence of high-risk clones ST235 and ST654, which are important vectors of MBL and ESBL and are associated with multidrug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Ines Leao, Leron Khalifa, Nicolas Gallois, Ivone Vaz-Moreira, Uli Kluemper, Daniel Youdkes, Shaked Palmony, Lotan Dagai, Thomas U. Berendonk, Christophe Merlin, Celia M. Manaia, Eddie Cytryn
Summary: Antibiotic-resistant bacteria and antibiotic resistance gene (ARGs) loads dissipate through sewage treatment plants to receiving aquatic environments. The removal of ARGs in the MABR is mainly a consequence of treatment-facilitated biomass removal, whereas in the stabilization reservoir, mitigation is linked to natural attenuation associated with ecosystem functioning. The study demonstrates the importance of ecosystem functioning in removing microbial contaminants from wastewater.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Helena M. Amaro, Joana F. Sousa, Eva M. Salgado, Jose C. M. Pires, Olga C. Nunes
Summary: This study assessed the ability of Chlorella vulgaris and UWW autochthonous microalgal species (AMS) to simultaneously remove PO4-P, and reduce the proliferation of coliforms and ARGs. Results showed that AMS performed better in all aspects, indicating its potential as an effective final treatment for urban wastewater.
APPLIED SCIENCES-BASEL
(2023)
Article
Environmental Sciences
Catarina Ferreira, Lara Luzietti, Sara Ribeirinho-Soares, Olga C. Nunes, Ivone Vaz-Moreira, Celia M. Manaia
Summary: This study compared the behavior of a clinical and an environmental K. pneumoniae strain harboring the blaKPC-3 gene in water. The abundance of the genes phoE and blaKPC-3 was monitored in urban runoff water and sterile ultra-pure water microcosms to assess survival, blaKPC-3 persistence, and the effect of the water microbiota. The results showed that K. pneumoniae and the blaKPC-3 gene decreased in urban runoff water, indicating competition with native microbiota, but the bacteria could still survive for at least one week.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Andre Torres-Pinto, Inmaculada Velo-Gala, Sara Ribeirinho-Soares, Olga C. Nunes, Claudia G. Silva, Joaquim L. Faria, Adrian M. T. Silva
Summary: Graphitic carbon nitride (GCN) is a promising optical semiconductor with excellent photoactivity under visible light irradiation. This study successfully improved the photocatalytic and electrocatalytic activities of GCN through thermal and chemical modification, leading to efficient organic micropollutant removal and microorganism inactivation in contaminated water.
ENVIRONMENTAL RESEARCH
(2023)
Review
Environmental Sciences
Helena M. Amaro, Eva M. Salgado, Olga C. Nunes, Jose C. M. Pires, Ana F. Esteves
Summary: Water, the most valuable resource, faces threats from anthropogenic activities that generate high levels of pollutants. Conventional wastewater treatment systems are not efficient in removing these pollutants. Microalgal systems have been seen as an environmentally friendly solution to remove pollutants, antibiotics, coliforms, and antibiotic resistance genes. Recent studies have evaluated the effectiveness of microalgal systems in various types of wastewater treatment.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Environmental Sciences
Pedro H. Presumido, Sara Ribeirinho-Soares, Rosa Montes, Jose Benito Quintana, Rosario Rodil, Marta Ribeiro, Teresa Neuparth, Miguel M. Santos, Manuel Feliciano, Olga C. Nunes, Ana I. Gomes, Vitor J. P. Vilar
Summary: A membrane ozone contactor was used for the tertiary treatment of urban wastewater, focusing on the removal of emerging contaminants, disinfection, and toxicity reduction. The system utilized a borosilicate tubular membrane coated with titanium dioxide and four UV lamps for radiation. By optimizing ozone concentration and gas flow rate, high removal rates of contaminants and disinfection efficacy were achieved. Antibiotic resistant bacteria were reduced but not eliminated after ozone treatment, and subsequent granular activated carbon adsorption further improved the water quality.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Joaquin A. Marrero, Rui S. Ribeiro, Sara Ribeirinho-Soares, Marta Pedrosa, Adrian M. T. Silva, Olga C. Nunes
Summary: This study demonstrates the suitability of carbon-based catalytic membranes for persulfate activation and water disinfection in continuous flow mode. The PVDF membranes with and without nitrogen-doped reduced graphene oxide (rGO-M) were tested. The rGO-M-PVDF membrane showed better microbial removal efficiency and persulfate activation capacity compared to the pristine PVDF membrane. It can effectively remove harmful microbiota in drinking water production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Environmental
Joana Abreu-Silva, Sara Ribeirinho-Soares, Ines Oliveira-Inocencio, Marta Pedrosa, Adrian M. T. Silva, Olga C. Nunes, Celia M. Manaia
Summary: This study investigated the performance of three types of filtering membranes (PC, CN, and PES) for bacterial analysis in demineralized and disinfected waters. CN or PES membranes required shorter filtration periods compared to PC membranes, although they had slightly lower DNA extraction yields. However, there were no significant differences in the abundance of genes per volume of water. CN membranes are a cost-effective alternative for analyzing waters with low microbial loads.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Environmental
A. Margarida Teixeira, Ivone Vaz-Moreira, David Calderon-Franco, David Weissbrodt, Sabina Purkrtova, Stanislav Gajdos, Giulia Dottorini, Per Halkjaer Nielsen, Leron Khalifa, Eddie Cytryn, Jan Bartacek, Celia M. Manaia
Summary: Urban wastewater treatment plants (UWTPs) are crucial for reducing pollutant load and protecting water bodies, but they also emit antibiotic resistant bacteria and resistance genes, which have impacts on downstream environments. This study tested a set of biomarkers for antibiotic resistance monitoring and found that qPCR can be a cost-effective and technically accessible approach for monitoring wastewater and downstream environments.