4.5 Article

High-throughput antibody-based assays to identify and quantify radiation-responsive protein biomarkers

Journal

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Volume 86, Issue 4, Pages 321-328

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.3109/09553000903564034

Keywords

radiation; biodosimeter; immunoassays; ELISA; cytokines; IL6

Funding

  1. NCI NIH HHS [P01 CA049062, CA 49062] Funding Source: Medline
  2. NIAID NIH HHS [U19 AI067773] Funding Source: Medline

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Material and methods: Ten normal human cell lines were screened by enzyme-linked immunosorbent assay (ELISA) for the expression of a dozen secreted cytokines that have been reported to have changes in protein or mRNA levels at 1, 2, and 3 days after 0-10 Gy irradiation using

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