Journal
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Volume 84, Issue 6, Pages 467-486Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09553000802078388
Keywords
Caco-2 cells; 2 Gy X-rays; latex microparticles; transepithelial resistance (TER); tight junctions; cell dimensions
Funding
- MRC [G0700730, MC_U142760473] Funding Source: UKRI
- Medical Research Council [G0700730, MC_U142760473] Funding Source: Medline
Ask authors/readers for more resources
Purpose:To determine the interaction between X-irradiation and invitro intestinal microparticle uptake through Caco-2 epithelial cells. Methods:Caco-2 cells were cultured on 3 m porous membranes for 21 days, X-irradiated with 2 Gy or sham-irradiated, then incubated for 5 or 30min and exposed apically for 30min to 2 m latex microparticles. Measurements included cell dimensions, from confocal microscope 'optical slices'; transepithelial resistance (TER) for tight junction (TJ) permeability; particle aggregation; and particle numbers on (adsorbed), in (intraepithelial) and through (submembranous) the epithelium. Results:Irradiation alone reduced TJ permeability more than sham-treatment, more so 5min than 30min after treatment. Irradiated epithelia were more permeable to particles than the equivalent sham-irradiated or previously untreated (particle only) groups: the latter two were similar. Irradiation altered adsorbed particle numbers and increased submembranous counts: particle uptake correlated best with cell height. Conclusions:2 Gy X-irradiation increased particle uptake and translocation through the epithelium. This correlated well with the TJ opening seen after particle exposure in irradiated samples and changes in cell morphology. New data on cell dimensions underlined the similarity in particle uptake between this invitro epithelium and that in an invivo model, highlighting the translational significance of the work.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available