Article
Plant Sciences
Srijan Jhingan, Avneesh Kumar, Hans-Joachim Harloff, Felix Dreyer, Amine Abbadi, Katrin Beckmann, Christian Obermeier, Christian Jung
Summary: Induced mutations are important sources of genetic variation in plant breeding. In this study, a rapeseed M-2 population was obtained by treating the parent cultivar 'Express' with EMS, and whole genomes were sequenced from 497 M-2 families. The mutation frequencies varied across chromosomes, and the majority of detected mutations were canonical transitions characteristic of EMS mutagenesis. The sequenced resource described in this study provides valuable information for functional gene studies in rapeseed breeding.
Article
Plant Sciences
Jared M. Simons, Tim C. Herbert, Coleby Kauffman, Marc Y. Batete, Andrew T. Simpson, Yuka Katsuki, Dong Le, Danielle Amundson, Elizabeth M. Buesche, Clifford Weil, Mitch Tuinstra, Charles Addo-Quaye
Summary: This study focuses on improving the accuracy of detecting EMS-induced mutations in a mutant population by implementing new methods, demonstrating high concordance between different variant-calling algorithms, and uncovering additional false-negative mutations. The final dataset contains a substantial increase in SNP detection, providing a valuable genetic resource for sorghum research.
Article
Genetics & Heredity
Zhe Yan, Michela Appiano, Ageeth van Tuinen, Fien Meijer-Dekens, Danny Schipper, Dongli Gao, Robin Huibers, Richard G. F. Visser, Yuling Bai, Anne-Marie A. Wolters
Summary: Three SlMLO genes in tomato act as susceptibility genes for powdery mildew, with SlMLO1 playing a major role due to a natural mutant allele ol-2. A novel allele m200 was identified in the SlMLO1 gene, conferring profound resistance to powdery mildew. The mutant allele carries a point mutation resulting in premature stop codon, providing a valuable genetic resource for powdery mildew resistance breeding.
Review
Plant Sciences
Miriam Szurman-Zubrzycka, Marzena Kurowska, Bradley J. Till, Iwona Szarejko
Summary: Since its introduction in 2000, the TILLING strategy has been widely used in plant research to create novel genetic diversity. TILLING mutants may be used for functional analysis of genes and being nontransgenic, they may be directly used in pre-breeding programs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agricultural Engineering
Jinesh Patel, Rahul Chandnani, Sameer Khanal, Jeevan Adhikari, Nino Brown, Peng W. Chee, Don C. Jones, Andrew H. Paterson
Summary: This study improved cotton fiber quality by using chemically induced mutants, enhancing traits such as fiber length, strength, and uniformity. The mutants showed potential for fiber quality beyond the levels of current elite varieties.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Ming Li Wang, Brandon Tonnis, Xianran Li, John Bradly Morris
Summary: Sesame is an important oilseed crop with natural genetic variation. The identification and utilization of genetic allele variation from the germplasm collection can improve seed quality.
Article
Energy & Fuels
Yimeng Lin, Yunye Zhang, Yang Fan, Chang Li, Hongzhi Ling, Wenxiang Ping, Jingping Ge
Summary: The study investigated the allocation of carbon flow from starch to lipid synthesis in algal cells. A mutant strain showed enhanced lipid content and productivity, as well as a favorable fatty acid composition for biodiesel production.
BIOENERGY RESEARCH
(2023)
Article
Plant Sciences
Karen Thulasi Devendrakumar, Madeleine Goldstein, James Kronstad, Xin Li
Summary: Agrobacterium-mediated transformation is a method that randomly inserts T-DNA into plant genomes, but this insertion may disrupt gene function. We report a phenomenon of reversion of intronic T-DNA mutant phenotypes, highlighting the need for caution when using such mutants and emphasizing the importance of using irreversible null mutants in genetic analysis.
Article
Plant Sciences
Natsume Koshika, Naohiro Shioya, Takashi Fujimura, Rina Oguchi, Chie Ota, Emi Kato, Reiko Takahashi, Shuichi Kimura, Shinsuke Furuno, Koichi Saito, Kazuhiro Okabe, Masanori Watanabe, Tomoki Hoshino
Summary: Induced mutation is an effective breeding strategy widely used in the development of elite plant varieties. In this study, a novel mutant population of soybeans was constructed using ethyl methanesulfonate to improve the variety of edamame. The TILLING method was employed to identify DNA mutations in the mutant populations, leading to the discovery of mutants with early flowering and harvest dates. These mutants can serve as DNA markers and are detectable using high-resolution melting analysis.
Article
Biochemistry & Molecular Biology
Dongzhi Wang, Yongpeng Li, Haojie Wang, Yongxin Xu, Yiman Yang, Yuxin Zhou, Zhongxu Chen, Yuqing Zhou, Lixuan Gui, Yi Guo, Chunjiang Zhou, Wenqiang Tang, Shuzhi Zheng, Lei Wang, Xiulin Guo, Yingjun Zhang, Fa Cui, Xuelei Lin, Yuling Jiao, Yuehui He, Junming Li, Fei He, Xigang Liu, Jun Xiao
Summary: In this study, a library for the popular wheat variety KN9204 was created using EMS mutagenesis, providing a comprehensive understanding of its genome, transcriptome, and epigenome. Exome capture sequencing revealed a high mutation rate in coding genes, with an average of 1383 EMS-type SNPs per line. New allelic variations for important agronomic trait-related genes were identified, and altered sensitivity to drought and salinity stress was observed in certain NAC transcription factors. This research provides valuable resources for functional genomics research and genetic manipulation of wheat.
PLANT COMMUNICATIONS
(2023)
Article
Agronomy
Zhou Zhou, Naoufal Lakhssassi, Dounya Knizia, Mallory A. Cullen, Abdelhalim El Baz, Mohamed G. Embaby, Shiming Liu, Oussama Badad, Tri D. Vuong, Amer AbuGhazaleh, Henry T. Nguyen, Khalid Meksem
Summary: The soybean acyl-ACP thioesterase gene family was characterized, leading to the discovery of mutants that can alter the fatty acid content of soybeans. These mutants provide a potential avenue for improving soybean oil composition.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Agronomy
Shaomei Wang, Xinru Wu, Yongfeng Guo, Dawei Wang, Lirui Cheng, Yuanying Wang, Aiguo Yang, Guanshan Liu
Summary: The study characterized a mutant of tobacco with spiral leaves and dwarfing phenotype, which displayed wrinkled, spiral, and miniature leaves and shortened internodes. Through gene mapping and genetic distance analysis, the mutant gene was located and the inheritance pattern was determined.
Article
Biochemistry & Molecular Biology
Xiaoxue Sun, Xing Li, Yin Lu, Shan Wang, Xiaomeng Zhang, Kang Zhang, Xiangjie Su, Mengyang Liu, Daling Feng, Shuangxia Luo, Aixia Gu, Yu Fu, Xueping Chen, Shuxin Xuan, Yanhua Wang, Donghui Xu, Shumin Chen, Wei Ma, Shuxing Shen, Feng Cheng, Jianjun Zhao
Summary: By utilizing a large-scale EMS mutant collection of Chinese cabbage, we successfully identified genes associated with yellow leaf mutant traits and variations in glucosinolate content, and established the Chinese cabbage EMS mutation database. This will facilitate gene mining of Chinese cabbage and the study of other Brassica crops.
Article
Agronomy
Boluwatife M. OlaOlorun, Hussein A. Shimelis, Isack Mathew
Summary: Genetic variation in wheat plays a crucial role in breeding for drought tolerance. This study evaluated genetic variation among 180 M-3 mutant families of wheat and selected top performing families for improved drought tolerance and biomass allocation to roots. Significant genotypic variation was observed for yield and biomass traits, highlighting the importance of genetic diversity in plant breeding programs.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Mason J. Appel, Scott A. Longwell, Maurizio Morri, Norma Neff, Daniel Herschlag, Polly M. Fordyce
Summary: New high-throughput biochemistry techniques provide quantitative kinetic and thermodynamic data for thousands of protein variants in parallel, requiring mutant libraries that are currently difficult to produce at needed scale and specificity. The approach presented in this study, uPIC-M, offers a scalable, rapid, and inexpensive solution for creating such libraries using recent advances in oligo synthesis, high-throughput sample preparation, and next-generation sequencing.