Article
Biochemistry & Molecular Biology
Sarah G. Cook, Nicole L. Rumian, K. Ulrich Bayer
Summary: CaMKII T286 autophosphorylation is required for both forms of hippocampal LTD, but with differential requirements for heterosynaptic communication of excitatory signals to inhibitory synapses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Neurosciences
Archana G. Mohanan, Sowmya Gunasekaran, Reena Sarah Jacob, R. Omkumar
Summary: Glutamatergic synapses contain abundant Ca2+/calmodulin-dependent protein kinase type II (CaMKII) with multiple functions, including kinase activity and binding to membrane proteins. The abundance of CaMKII in synapses is similar to scaffolding proteins, but its prominent function is still as a kinase. The multimeric structure of CaMKII enables it to have various functional capabilities, such as Ca2+ signal transduction, memory molecule function, and scaffolding. This article reviews the multiple roles of CaMKII in glutamatergic synapses and how they are affected in disease conditions.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Anastasiia Bohush, Wieslawa Lesniak, Serge Weis, Anna Filipek
Summary: Parkinson's disease is a neurodegenerative disorder characterized by the formation of Lewy bodies and loss of dopaminergic neurons. Changes in intracellular Ca2+ levels can have serious consequences on cells, with calmodulin playing a crucial role in regulating neuronal plasticity. Therefore, alterations in the activity of calmodulin and its binding proteins may be linked to the development and progression of PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ohm Prakash, Marie Held, Liam F. McCormick, Nitika Gupta, Lu-Yun Lian, Svetlana Antonyuk, Lee P. Haynes, N. Lowri Thomas, Nordine Helassa
Summary: This study reveals the molecular mechanisms of CaM variants associated with catecholaminergic polymorphic ventricular tachycardia (CPVT), providing new insights for the development of future therapeutic strategies.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Jaeho Yoon, Jian Sun, Moonsup Lee, Yoo-Seok Hwang, Ira O. Daar
Summary: Apical constriction is a crucial cell shape change for neural tube closure, and it is driven by actin-myosin networks. The WERDS complex, consisting of Wnt4, ephrinB2, Ror2, Dsh2, and Shroom3, plays an instructive role in this process. Disruption of the WERDS complex impairs apical constriction and neural tube closure, and the mechanism involves the interaction of ephrinB2 with Dsh2 to activate Rho-associated kinase and induce apical constriction. Furthermore, the ephrinB2/Dsh2 interaction promotes non-canonical Wnt signaling and coordinates the morphogenesis of the neural tube through crosstalk with the Eph/ephrin pathway.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Monika Jozsa, Tihol Ivanov Donchev, Rodolphe Sepulchre, Timothy O'Leary
Summary: Research has shown that a self-regulating signaling motif can generate reliable size-regulated switches by exploiting the breakdown of mass-action kinetics in chemical reaction systems. In synaptic and other small compartments, fluctuations are large due to low copy numbers of species, leading to a different behavior than typical mass-action kinetics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Omid Haji-Ghassemi, Yu Seby Chen, Kellie Woll, Georgina B. Gurrola, Carmen R. Valdivia, Wenxuan Cai, Songhua Li, Hector H. Valdivia, Filip Van Petegem
Summary: Calcins are peptides from scorpion venom that can cross cell membranes and target intracellular ion channels called Ryanodine Receptors (RyR). A study using cryo-electron microscopy found that imperacalcin, a type of calcin, binds to RyR and opens the channel pore, resulting in asymmetry and extended ion conduction pathways. Phosphorylation of imperacalcin by protein kinase A prevents its binding to RyR, highlighting the role of posttranslational modifications in determining the fate of a natural toxin. The structure of imperacalcin provides a template for developing analogs with potential to treat RyR-related disorders.
Article
Neurosciences
Zi-Fan Zhuang, Hong-Yun Wu, Ya-Yi Song, Lei Li, Xia Cui, Jie Yang, Xiang-Qing Xu, Wen-Qiang Cui
Summary: The activation of NMDA/CaMKII signaling in the lateral habenula (LHb) is involved in the occurrence and development of trigeminal neuralgia (TN) and related anxiety-like behaviors. Targeting NMDA/CaMKII to suppress the activity of CaMKII+ neurons in the bilateral LHb may represent a novel strategy for treating pain and anxiety associated with TN.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Andrew C. Hedman, Zhigang Li, Laetitia Gorisse, Swetha Parvathaneni, Chase J. Morgan, David B. Sacks
Summary: AMP-activated protein kinase (AMPK) is crucial in maintaining cellular energy homeostasis through regulating catabolic and anabolic pathways, with IQGAP1 identified as a novel regulator of AMPK signaling. Lack of IQGAP1 leads to impaired AMPK activation and metabolic dysregulation, highlighting the importance of the functional interplay between IQGAP1 and AMPK.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Yixi Liu, Qun Shao, Heng-Jie Cheng, Tiankai Li, Xiaowei Zhang, Michael F. Callahan, David Herrington, Dalane Kitzman, David Zhao, Che-Ping Cheng
Summary: CaMKII inhibition can improve cardiomyocyte function and [Ca2+](i) regulation in patients with CHF, thereby enhancing the cardiac beta-adrenergic reserve.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2021)
Article
Pharmacology & Pharmacy
Yun Ling, Jiajun Shi, Quanxin Ma, Qinqin Yang, Yili Rong, Jiangmin He, Minli Chen
Summary: GXNT exerts vasodilatory effects by activating the endothelial CaMKII/eNOS signaling pathway and regulating calcium-related ion channels.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
B. Zhou, Y. Lin, S. Chen, J. Cai, Z. Luo, S. Yu, J. Lu
Summary: This study found that lidocaine induced pyroptosis of U87-MG glioblastoma cells by activating CaMKII, leading to calcium overload and upregulating caspase-3 and GSDME proteins.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Qixu Cai, Xiumin Chen, Shihan Zhu, Roger A. Nicoll, Mingjie Zhang
Summary: Calcium calmodulin-dependent kinase II (CaMKII) is crucial for synaptic transmission and plasticity. The length of the unstructured linker between the kinase domain and the oligomerizing hub plays a crucial role in determining the ability of CaMKII to rescue defects in synaptic transmission and long-term potentiation. The phase separation capacity of CaMKII with GluN2B is critical for its cellular functions in the brain.
Article
Biochemistry & Molecular Biology
Nathalia Rocco-Machado, Lo Lai, Geumsoo Kim, Yi He, Elizabeth D. Luczak, Mark E. Anderson, Rodney L. Levine
Summary: Calcium/calmodulin-dependent protein kinase II 8 (CaMKII8) plays a pivotal role in cardiac signaling, and oxidative stress can induce its autonomous activation. This study demonstrates that covalent oxidation leads to the formation of a disulfide bond between Cys273 and Cys290 in the regulatory domain, causing autonomous activity. Additionally, the MMVV mutant of CaMKII protects the heart from oxidative stress-induced cardiac decompensation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Maria Florencia Racioppi, Juan Ignacio Burgos, Malena Morell, Luis Alberto Gonano, Martin Vila Petroff
Summary: The study compared the cardiotoxic effects of classic pharmacologic Na+/K+ ATPase inhibition versus istaroxime. Results showed that istaroxime did not activate calcium/calmodulin-dependent kinase II or lead to cardiomyocyte death, in contrast to ouabain. Additionally, istaroxime had a lower impact on cardiac Ca2+ handling, suggesting a unique mechanism for its low arrhythmogenic profile.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Biochemical Research Methods
Mukesh Kumar, Shai R. Joseph, Martina Augsburg, Aliona Bogdanova, David Drechsel, Nadine L. Vastenhouw, Frank Buchholz, Marc Gentzel, Andrej Shevchenko
MOLECULAR & CELLULAR PROTEOMICS
(2018)
Article
Biochemistry & Molecular Biology
D. Mielenz, M. Reichel, T. Jia, E. B. Quinlan, T. Stoeckl, M. Mettang, D. Zilske, E. Kirmizi-Alsan, P. Schoenberger, M. Praetner, S. E. Huber, D. Amato, M. Schwarz, P. Purohit, S. Brachs, J. Spranger, A. Hess, C. Buettner, A. B. Ekici, F. Perez-Branguli, B. Winner, V. Rauschenberger, T. Banaschewski, A. L. W. Bokde, C. Buechel, P. J. Conrod, S. Desrivieres, H. Flor, V. Frouin, J. Gallinat, H. Garavan, P. Gowland, A. Heinz, J-L Martinot, H. Lemaitre, F. Nees, T. Paus, M. N. Smolka, A. Schambony, T. Baeuerle, V. Eulenburg, C. Alzheimer, A. Lourdusamy, G. Schumann, C. P. Mueller
MOLECULAR PSYCHIATRY
(2018)
Article
Cell Biology
Dominic B. Bernkopf, Kowcee Jalal, Martina Brueckner, Karl X. Knaup, Marc Gentzel, Alexandra Schambony, Juergen Behrens
JOURNAL OF CELL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Maria Kotini, Elias H. Barriga, Jonathan Leslie, Marc Gentzel, Verena Rauschenberger, Alexandra Schambon, Roberto Mayor
NATURE COMMUNICATIONS
(2018)
Correction
Multidisciplinary Sciences
Maria Kotini, Elias H. Barriga, Jonathan Leslie, Marc Gentzel, Verena Rauschenberger, Alexandra Schambony, Roberto Mayor
NATURE COMMUNICATIONS
(2018)
Article
Biochemical Research Methods
Marc Gentzel, Mercedes Pardo, Sivaraman Subramaniam, A. Francis Stewart, Jyoti S. Choudhary
Article
Optics
Richard W. Taylor, Reza Gholami Mahmoodabadi, Verena Rauschenberger, Andreas Giessl, Alexandra Schambony, Vahid Sandoghdar
Article
Multidisciplinary Sciences
Jakub Harnos, Maria Consuelo Alonso Canizal, Miroslav Jurasek, Jitender Kumar, Cornelia Holler, Alexandra Schambony, Katerina Hanakova, Ondrej Bernatik, Zbynek Zdrahal, Kristina Gomoryova, Tomas Gybel, Tomasz Witold Radaszkiewicz, Marek Kravec, Lukas Trantirek, Jan Rynes, Zankruti Dave, Ana Iris Fernandez-Llamazares, Robert Vacha, Konstantinos Tripsianes, Carsten Hoffmann, Vitezslav Bryja
NATURE COMMUNICATIONS
(2019)
Article
Biology
Janelle Lauer, Sandra Segeletz, Alice Cezanne, Giambattista Guaitoli, Francesco Raimondi, Marc Gentzel, Vikram Alva, Michael Habeck, Yannis Kalaidzidis, Marius Ueffing, Andrei N. Lupas, Christian Johannes Gloeckner, Marino Zerial
Article
Immunology
Benjamin W. Dorschner, Ralf Wiedemuth, Ann-Christin Funke, Marc Gentzel, Mary-Louise Rogers, Sebastian Brenner, Sebastian Thieme
Summary: This study introduces a comprehensive workflow and an optimized IP protocol for investigating neurotrophin receptor p75NTR at endogenous, low abundance levels, providing detailed instructions from lysate preparation to successful detection of protein-protein interactions.By utilizing human melanoma cell line A375 and human leukemic plasmacytoid dendritic cell line PMDC05, specific antibodies and IP conditions were validated, revealing 14-3-3 epsilon:p75NTR interactions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Alastair W. Skeffington, Marc Gentzel, Andre Ohara, Alexander Milentyev, Christoph Heintze, Lorenz Boettcher, Stefan Goerlich, Andrej Shevchenko, Nicole Poulsen, Nils Kroeger
Summary: This study extracted silica proteins from three diatom species and found that there were no common proteins among them, and most proteins showed low similarity in sequence alignments. Bioinformatic analysis grouped these proteins into different classes based on unconventional sequence motifs, but their functions are still unclear. In vivo localization results of selected proteins suggest that proteins lacking sequence homology but sharing unconventional sequence motifs may have similar functions in diatom silica cell wall morphogenesis.