Cytocompatibility of Poly(L-lactide-co-glycolide) Porous Scaffold Materials for Tissue Engineering

The development of a suitable three-dimensional scaffold for the maintenance of cellular viability and differentiation is critical for applications in periodontal tissue engineering. Poly(L-lactide-co-glycolide) (PLGA) porous scaffold materials were fabricated by using NaHCO(3) particulates as progen, and the compatibility test in vitro was carried out. The scaffold shows an interconnected structure with large pore sizes ranging from 100 to 450 mu m. Cells did attach to the culture plate when cultured in PLGA extract, and the number of cells increased with the increase of culture time. Von Kossa-positive mineralization nodules were first observed after 21 days of culture time. The percent of viable cells cultured in PLGA extract was higher than that in PLLA extract at the same culture period. Nodule formation and multilayer structures were observed on the scaffold surface.