Article
Chemistry, Multidisciplinary
David C. C. Luther, Ritabrita Goswami, Yi-Wei Lee, Taewon Jeon, Rui Huang, James L. L. Elia, Harini Nagaraj, Jetta J. E. Bijlsma, Martin Piest, Martijn A. A. Langereis, Vincent M. M. Rotello
Summary: Vaccination through cellular transfection of nucleotide-based vaccines using versatile guanidinium-functionalized poly(oxanorbornene)imide (PONI-Guan) homopolymers as vectors has shown strong immune responses against Newcastle disease virus (NDV) in white leghorn chickens. This approach presents a highly flexible method for targeted immunomodulation with translatability potential as a non-viral vaccine platform.
Article
Biotechnology & Applied Microbiology
Aishwarya Saraswat, Ketan Patel
Summary: This study aimed to find the optimal lipid-based nanovector for gene delivery by testing various types. The study found that lipid nanoparticles (LNPs) were more effective at delivering plasmid DNA than nanocomplexes and also provided better protection. The best performing LNP contained the biodegradable lipid sphingomyelin 102. Therefore, the optimized LNP formulation using sphingomyelin 102 could be a highly efficient nonviral gene-delivery system for targeting drug-resistant melanoma.
Article
Chemistry, Physical
Sergey Stolyar, Roman N. Yaroslavtsev, Anna Tyumentseva, Sergey Komogortsev, Ekaterina S. Tyutrina, Alina T. Saitova, Yulia Gerasimova, Dmitriy A. Velikanov, Michael Rautskii, Rauf S. Iskhakov
Summary: Iron oxide nanoparticles were synthesized through coprecipitation method and the effects of different Fe3O4/tetraethoxysilane ratios on their properties were investigated. The results showed that the magnetite core in nanoparticles had higher magnetization compared to stoichiometric magnetite nanoparticles, which was caused by the interaction with the silicate coating. Additionally, the thickness of the nanoparticle shell was found to be related to the efficiency of DNA isolation.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Physical
Paurnima Talele, Anand Jadhav, Shivaji Tayade, Saugata Sahu, Kiran Kumar Sharma, Navinchandra Shimpi
Summary: Solid lipid nanoparticles (SLNs) loaded with hydroquinone (HQ) were successfully prepared using stearic acid and cationic/anionic emulsifiers. The physicochemical characterization of SLNs was performed, and the release behavior of HQ from SLNs was studied using an electrochemical method. The results showed that the concentration of emulsifier had a significant impact on the encapsulation and release of HQ.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Medicine, Research & Experimental
Jaidev Chakka, Mohammed Maniruzzaman
Summary: In this study, a novel extrusion-based Sprayed Multi Adsorbed-droplet Reposing Technology (SMART) was used to prepare 150 nm plasmid DNA nanoparticles, which were successfully applied in vitro transfection experiments with cancer cells.
MOLECULAR PHARMACEUTICS
(2023)
Article
Microbiology
Wenyue Hu, Yuli Wang, Bingxu Yang, Chen Lin, Hao Yu, Guang Liu, Zixin Deng, Hong-Yu Ou, Xinyi He
Summary: SRAP is an ancient protein superfamily found in various domains of life. YedK has a high affinity to DNA duplex with single strand segments. Mutants of YedK in Escherichia coli do not show differences in response to UV and DNA damaging reagents, indicating its non-essentiality or redundancy in E. coli.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Polymer Science
Joana S. Correia, Sofia Miron-Barroso, Charlotte Hutchings, Silvia Ottaviani, Birsen Somuncuoglu, Leandro Castellano, Alexandra E. Porter, Jonathan Krell, Theoni K. Georgiou
Summary: The influence of polymer architecture on cytotoxicity was investigated in this study. It was found that block-like polymer structures had lower cytotoxicity compared to statistical or gradient/tapered architectures. These findings have implications for the development of nucleic acid delivery vectors.
Article
Engineering, Biomedical
Yue Qin, Adam A. Walters, Nadia Rouatbi, Julie Tzu-Wen Wang, Hend Mohamed Abdel-Bar, Khuloud T. Al-Jamal
Summary: This study established a model for predicting the effects of different lipid compositions on particle size, TNA encapsulation, and transfection efficiency using a minimum run of 24 SNALP formulations. The results showed that particle size, in vitro and in vivo transfection efficiency, and encapsulation efficiency were affected by lipid compositions. However, in vitro transfection efficiency could not predict in vivo transfection effects. The Design of Experiments approach described in this study can provide comprehensive optimization of LNPs for various applications.
Article
Chemistry, Multidisciplinary
Takeshi Terada, Jayesh A. Kulkarni, Ariel Huynh, Sam Chen, Roy van der Meel, Yuen Yi C. Tam, Pieter R. Cullis
Summary: This study investigated the impact of preparation parameters on the physicochemical properties of LNP-siRNA systems, finding that lipid concentration and flow rate ratio significantly affected particle size and PDI, while dialysis resulted in a decrease in PDI and an increase in particle size.
Article
Pharmacology & Pharmacy
Wei Xu, Zhaoyou Deng, Yifei Xiang, Dujuan Zhu, Dandan Yi, Yihao Mo, Yu Liu, Lanqian Qin, Ling Huang, Bingjie Wan, Liqin Wu, Xin Feng, Jiakang He
Summary: In this study, a tolfenamic acid solid lipid nanoparticle (TA-SLN) suspension was prepared to improve the sustained release and bioavailability of nonsteroidal anti-inflammatory drugs. The TA-SLN suspension showed improved sustained drug release in vitro and increased pharmacokinetic parameters in pigs compared with TA injection, indicating its potential as a promising new formulation.
Article
Chemistry, Multidisciplinary
Pabitra Mondal, Joykrishna Dey, Sadhana Roy, Somdeb Bose Dasgupta
Summary: Bolaamphiphiles or bolaforms have drawn attention in drug and gene delivery, and this study focuses on the development of biodegradable cationic bolaforms with improved gene transfection ability. The self-assembly behavior of a new cationic bolaform (Bola-1) with hydroxyl (OH) groups was compared to its precursor molecule's hydrochloride salt (Bola-2). Both bolaforms were found to form micelles at low critical aggregation concentrations (CAC), with the presence of OH groups significantly affecting the CAC value. Despite higher binding efficiency with DNA, both Bola-1 and Bola-2 showed comparable gene transfection efficiency to turbofectamine standard in vitro. Additionally, both bolaforms exhibited significant cytotoxicity and beneficial antibacterial activity at higher concentrations.
Article
Pharmacology & Pharmacy
Yue Qin, Adam A. Walters, Khuloud T. Al-Jamal
Summary: This study compared the transfection efficiency and cytotoxicity of three non-viral vectors (PEI, LPF, and SNALPs) for delivering pDNA expressing immunostimulatory molecules. PEI and LPF showed high transfection efficiency but upregulated PD-L1 expression and induced cytotoxicity. SNALPs, on the other hand, had lower transfection efficiency but did not upregulate PD-L1 expression or induce cytotoxicity, making them a viable non-viral vector for pDNA delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Chemistry, Multidisciplinary
Arne Matteo Joergensen, Richard Wibel, Andreas Bernkop-Schnuerch
Summary: Cationic and ionizable cationic lipids have been widely used as auxiliary agents with associated adverse effects. However, their toxic potential can be minimized if they are rapidly degraded to endogenously occurring metabolites. Biodegradable cationic and ionizable cationic lipids have shown promising results in terms of functionality and safety. This review provides an overview of these lipids, including their synthesis, cleavage by endogenous enzymes, toxicity, application in drug delivery systems, and use as preservatives, as well as guidance for their design and future developments.
Article
Biophysics
Annita Katopodi, Kyriaki Safari, Alexandros Kalospyros, Konstantinos Politopoulos, Eleni Alexandratou, Anastasia Detsi
Summary: In this study, daphnetin and its synthetic analogue DHC were encapsulated in solid lipid nanoparticles (SLNs) and showed enhanced antioxidant and photodynamic therapy (PDT) efficacy compared to their free form. The study also found that the cellular uptake of the coumarin analogues was improved when loaded in SLNs.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Engineering, Chemical
Yahya F. Jamous, Najla A. Altwaijry, Mohamed T. S. Saleem, Aljoharah F. Alrayes, Sara M. Albishi, Mashael A. Almeshari
Summary: This study focuses on troxerutin-loaded solid lipid nanoparticles (TXR-SLNs) and their physicochemical properties to enhance drug release. The TXR-SLNs exhibited optimized nanoparticles with improved drug-loading capacity and extended drug release. The study highlights the potential of SLNs as effective carriers and encourages further in vivo investigations to optimize therapeutic interventions.
Article
Cell Biology
Silvia Prieto-Sanchez, Cristina Moreno-Castro, Cristina Hernandez-Munain, Carlos Sune
JOURNAL OF CELL SCIENCE
(2020)
Review
Genetics & Heredity
Marc Sune-Pou, Maria J. Limeres, Cristina Moreno-Castro, Cristina Hernandez-Munain, Josep M. Sune-Negre, Maria L. Cuestas, Carlos Sune
FRONTIERS IN GENETICS
(2020)
Article
Chemistry, Physical
Ewelina Juszczyk, Piotr Kulinowski, Ewelina Baran, Artur Birczynski, Dorota Majda, Encarna Garcia-Montoya, Pilar Perez-Lozano, Josep Maria Sune-Negre, Wladyslaw P. Weglarz, Przemyslaw Dorozynski
Summary: The study aimed to develop methodology for spatiotemporal characterization of water transport and properties in alginate tablets under hydration. Sampling using different instruments provided key parameters of water content, nonfreezing pool percentage, and T-2 relaxation time in different hydration layers. A hybrid destructive/nondestructive analysis method for alginate matrices under hydration was proposed to assess temporal changes of water distribution and its interaction with matrices in identified layers.
Review
Pharmacology & Pharmacy
Khadija Rouaz, Blanca Chiclana-Rodriguez, Anna Nardi-Ricart, Marc Sune-Pou, Debora Mercade-Frutos, Josep Maria Sune-Negre, Pilar Perez-Lozano, Encarna Garcia-Montoya
Summary: This theoretical study critically reviews the use of excipients in paediatric medicine, emphasizing the importance of safety checks and toxicity studies. It identifies common excipients used in pediatric formulations and explores the orally disintegrating tablets, ODT, as a promising pharmaceutical form for children.
Article
Biochemistry & Molecular Biology
Laura Payan-Bravo, Sara Fontalva, Xenia Penate, Ildefonso Cases, Jose Antonio Guerrero-Martinez, Yerma Pareja-Sanchez, Yosu Odriozola-Gil, Esther Lara, Silvia Jimeno-Gonzalez, Carles Sune, Mari Cruz Munoz-Centeno, Jose C. Reyes, Sebastian Chavez
Summary: The study demonstrates that perturbations in prefoldin lead to transcriptional alterations in the human genome, particularly affecting splicing and expression of long genes with a high number of introns.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Marta Marquez Valls, Alejandra Martinez Labrador, Lyda Halbaut Bellowa, Domenica Bravo Torres, Paulo C. Granda, Montserrat Minarro Carmona, David Limon, Ana C. Calpena Campmany
Summary: Inflammatory processes in the oral cavity can be effectively treated with semisolid formulations containing active pharmaceutical ingredients such as triamcinolone acetonide and lidocaine hydrochloride. These formulations show potential for symptomatically treating painful buccal sores caused by diseases like diabetes, AIDS, and leukemia, demonstrating anti-inflammatory activity in both buccal and sublingual mucosa under dynamic conditions.
Article
Chemistry, Physical
Ewelina Juszczyk, Piotr Kulinowski, Ewelina Baran, Artur Birczynski, Jolanta Klaja, Dorota Majda, Encarna Garcia-Montoya, Wladyslaw P. Weglarz, Przemyslaw Dorozynski
Summary: The study demonstrated the unique hydration patterns of polymer-based matrices, showing features such as internal locking, lamellar regions, water resolution, and water penetration. These patterns were influenced by matrix composition and molecular properties of constituents.
Article
Pharmacology & Pharmacy
Lola Amoros-Galicia, Anna Nardi-Ricart, Clara Verdugo-Gonzalez, Carmen Martina Arroyo-Garcia, Encarna Garcia-Montoya, Pilar Perez-Lozano, Josep Ma Sune-Negre, Marc Sune-Pou
Summary: Although there is currently no standardized method for measuring bioadhesion/mucoadhesion, this study developed a single/systematic in vitro method that can be applied to various pharmaceutical dosage forms. By comparing the results of different formulations, the adhesive capacity of bioadhesive formulations was demonstrated. This method allows for comparisons of results across studies and facilitates the selection of suitable formulations and adhesive products based on their bioadhesive properties.
Article
Chemistry, Physical
Ronny Vargas, Miquel Romero, Tomas Berasategui, David A. Narvaez-Narvaez, Patricia Ramirez, Anna Nardi-Ricart, Encarna Garcia-Montoya, Pilar Perez-Lozano, Josep M. Sune-Negre, Cristina Moreno-Castro, Cristina Hernandez-Munain, Carlos Sune, Marc Sune-Pou
Summary: Manufacturing lipid nanoparticles using microfluidic mixing can be optimized by considering critical process parameters, including total flow, flow rate ratio, and dialysis. This study found that dialysis is a crucial parameter that strongly affects particle size and zeta potential, with moderate impact on polydispersity index. The relevance of flow rate ratio decreases when dialysis is performed. Purification should be integrated into the manufacturing process of lipid nanoparticles to account for the influence of other process parameters.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2023)
Article
Pharmacology & Pharmacy
Khadija Rouaz-El Hajoui, Helena Herrada-Manchon, David Rodriguez-Gonzalez, Manuel Alejandro Fernandez, Enrique Aguilar, Marc Sune-Pou, Anna Nardi-Ricart, Pilar Perez-Lozano, Encarna Garcia-Montoya
Summary: This paper presents the application of semi-solid extrusion 3D printing technology in developing patient-tailored medicinal gummies with an innovative omeprazole pharmaceutical form for paediatric use. The study compares the gastro-resistance and dissolution profiles of 3D printed hydrogels with omeprazole pellets, highlighting the effectiveness of 3D printed chewable semi-solid doses with enteric pellets in addressing the challenge of paediatric medication.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Alba Figuera-Figuera, Marc Sune-Pou, Pilar Perez-Lozano, Encarna Garcia-Montoya, Joaquim Amela-Navarro, Josep M. Sune-Negre
Summary: During the study, the manufacturing process of the drug Linezolid from three different sources was validated, and differences in critical material attributes between the API manufacturers were identified. The scalability factor and suitability for direct compression were also evaluated. The results showed that SeDeM methodology successfully validated the API manufacturing process, assessed scalability, and distinguished between sources, making it highly valuable for selecting the best API source in the formulation phase.
Article
Chemistry, Medicinal
David A. Narvaez-Narvaez, Maria Duarte-Ruiz, Sandra Jimenez-Lozano, Cristina Moreno-Castro, Ronny Vargas, Anna Nardi-Ricart, Encarna Garcia-Montoya, Pilar Perez-Lozano, Josep Ma Sune-Negre, Cristina Hernandez-Munain, Carlos Sune, Marc Sune-Pou
Summary: PEGylation is crucial in enhancing the physicochemical and biological characteristics of cholesteryl-oleate cationic solid-lipid nanoparticles (CO-cSLNs), resulting in small, stable, and non-toxic nanoparticles suitable for gene and RNA therapies.
Review
Biochemistry & Molecular Biology
Alonso Rodriguez-Caparros, Jesus Alvarez-Santiago, Maria Jesus del Valle-Pastor, Carlos Sune, Jennifer Lopez-Ros, Cristina Hernandez-Munain
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)