Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 448, Issue 1, Pages 19-27Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2013.03.015
Keywords
Alginate; Chitosan derivatives; Microparticles; Oral vaccine delivery; Ovalbumin
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Funding
- Silpakorn University Research and Development Institute
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The purpose of this study was to prepare microparticles entrapping ovalbumin (OVA) as a model antigen to induce immune responses in mice following oral vaccination. In this study, calcium-alginate and calcium-yam-alginate microparticles were prepared by crosslinking alginate with calcium chloride solution using an electrospraying technique. 0.1% (w/v) of methylated N-(4-N,N-dimethylaminocinnamyl) chitosan (TM65CM50CS) was used to coat microparticles entrapping an initial OVA of 20% w/w to polymer. The results indicated that the coated microparticles were spherical and had a smooth surface, with an average size of 1-3 mu m, and were positively charged. In addition, the particles demonstrated a greater swelling and mucoadhesive properties than did uncoated microparticles. The in vitro release from the microparticles indicated that the coated microparticles resulted in more sustained release than uncoated microparticles. The cytotoxicity results showed that all of the formulations were safe. The in vivo oral administration demonstrated that at the same amount of 250 mu g OVA, coated microparticles exhibited the highest in vivo adjuvant activity in both IgG and IgA immunogenicity. (C) 2013 Elsevier B.V. All rights reserved.
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