Article
Chemistry, Physical
Catarina Costa, Isabel S. Oliveira, Joao P. N. Silva, Sandra G. Silva, Claudia Botelho, M. Luisa C. do Vale, Maria Elisabete C. D. Real Oliveira, Andreia C. Gomes, Eduardo F. Marques
Summary: The study focused on developing nanovectors for siRNA delivery based on three gemini amino acid-based surfactants combined with MO as helper lipid. The systems showed efficient complexation of siRNA and high levels of gene silencing, with minimal cytotoxicity observed. These gemini:MO systems are promising candidates as lipofection vectors for RNA interference (RNAi)-based therapies.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Nataliya Gretskaya, Mikhail Akimov, Dmitry Andreev, Anton Zalygin, Ekaterina Belitskaya, Galina Zinchenko, Elena Fomina-Ageeva, Ilya Mikhalyov, Elena Vodovozova, Vladimir Bezuglov
Summary: The purpose of this study was to develop multi-component cationic lipid nanoparticles (LNPs) for efficient delivery of RNA to mammalian cells. The study found that LNPs containing DOTAP were more effective than those containing Ol-Ch. Core LNPs showed lower transfection activity compared to bilayer LNPs. The type of phospholipid in LNPs significantly affected the transfection of cancer cells but not normal cells, and LNPs containing GM3 gangliosides were the most efficient for delivering mRNA and siRNA.
Article
Pharmacology & Pharmacy
Erica Quagliarini, Serena Renzi, Luca Digiacomo, Francesca Giulimondi, Barbara Sartori, Heinz Amenitsch, Valentina Tassinari, Laura Masuelli, Roberto Bei, Lishan Cui, Junbiao Wang, Augusto Amici, Cristina Marchini, Daniela Pozzi, Giulio Caracciolo
Summary: Fine-tuning of formulation and microfluidic parameters plays a vital role in the generation of highly efficient DNA-loaded LNPs. Factors such as PEGylation and post-synthesis sample concentration facilitate the preparation of homogeneous LNPs with high transfection efficiency, while increasing total flow rate has a detrimental effect on the physicochemical properties and transfection properties of LNPs. The effect of particle concentration at the cell surface on transfection efficiency and cell viability varies depending on the cell line, indicating a need for case-by-case optimization.
Article
Materials Science, Multidisciplinary
Wen Song, Qingyuan Ye, Zhe Li, Yajie Hou, Lina Niu, Zhiwei Ma, Chuanxu Yang
Summary: This study reveals the significant impact of transfection medium on chitosan-mediated siRNA delivery, with even disturbed physical parameters of nanoparticles still achieving high knockdown efficiency.
MATERIALS & DESIGN
(2022)
Article
Polymer Science
Matthew R. Bockman, Rishad J. Dalal, Ramya Kumar, Theresa M. Reineke
Summary: This study presents an improved synthetic route to incorporate a GalNAc-derived monomer into polymers targeting hepatocytes, showing good performance in terms of toxicity, uptake, and transfection efficiency against liver cells.
Article
Pharmacology & Pharmacy
Claudia Lotter, Claudio Luca Alter, Jan Stephan Bolten, Pascal Detampel, Cornelia G. Palivan, Tomaz Einfalt, Jorg Huwyler
Summary: In this study, phosphatidylserine (PS) was incorporated into non-viral lipid nanoparticle (LNP) DNA/RNA constructs in a virus-inspired biomimetic approach to enhance cellular transfection. The results showed that PS-decorated LNPs exhibited significantly increased transgene expression in vitro and enhanced transfection efficiency in vivo. This method provides a new avenue for efficient gene therapy and can be extended to other therapeutic systems.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Materials Science, Multidisciplinary
Pengkai Wu, Shuai Yin, Tianqing Liu, Dan Ding, Kaikai Wang
Summary: By incorporating different functional ligands and linked reductive disulfides into low-molecular-weight PEI 1.8k, this study constructed reductive-responsive PEI-X-SS-X-PEI copolymers to enhance siRNA transfection efficiency. The strategy, named building-block crosslinking, showed a simple and smart way to synthesize new materials and improve gene delivery systems.
SCIENCE CHINA-MATERIALS
(2021)
Article
Multidisciplinary Sciences
Yuan Rui, David R. Wilson, Stephany Y. Tzeng, Hannah M. Yamagata, Deepti Sudhakar, Marranne Conge, Cynthia A. Berlinicke, Donald J. Zack, Anthony Tuesca, Jordan J. Green
Summary: Nanoparticle-based mRNA therapeutics show great promise, but cellular internalization and endosomal escape pose challenges for cytosolic delivery. Researchers developed a dual nanoparticle uptake and endosomal disruption assay, which can detect cellular internalization and endosomal disruption. The study found that this assay has higher predictive capacity for mRNA delivery compared to conventional polymer and nanoparticle characteristics.
Article
Genetics & Heredity
Fereydoon Abedi Gaballu, William Chi-Shing Cho, Gholamreza Dehghan, Amir Zarebkohan, Behzad Baradaran, Behzad Mansoori, Soheil Abbaspour-Ravasjani, Ali Mohammadi, Nader Sheibani, Ayuob Aghanejad, Jafar Ezzati Nazhad Dolatabadi
Summary: The study successfully delivered specific siRNA targeting HMGA2 using G4/MTX-siRNA nanocomplex, inducing apoptosis in breast cancer cells, suggesting a promising therapeutic strategy for breast cancer treatment.
Article
Biochemistry & Molecular Biology
Jixian Luo, Jiangfeng Chen, Yan Liu, Yongji He, Wenjuan Dong
Summary: The modification of low-molecular-weight chitosan with arginine resulted in the successful creation of arginine-chitosan nanoparticles. These nanoparticles were able to encapsulate and deliver siRNA safely to leukemia cells, making them a potential tool for future therapies targeting specific genes. This study identified arginine-chitosan nanoparticles for the first time and explored their ability to deliver Rhoa siRNA into T-cell acute lymphoblastic leukemia cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Erica Quagliarini, Junbiao Wang, Serena Renzi, Lishan Cui, Luca Digiacomo, Gianmarco Ferri, Luca Pesce, Valentina De Lorenzi, Giulia Matteoli, Heinz Amenitsch, Laura Masuelli, Roberto Bei, Daniela Pozzi, Augusto Amici, Francesco Cardarelli, Cristina Marchini, Giulio Caracciolo
Summary: Lipid nanoparticles (LNPs) have shown promise as effective gene delivery vehicles, with a specific formulation (LNP15) demonstrating successful transfection and potential application in cancer vaccines.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Junjie Pan, Chi-ling Chiang, Xinyu Wang, Paul Bertani, Yifan Ma, Junao Cheng, Vishank Talesara, Ly James Lee, Wu Lu
Summary: Nanochannel electroporation (NEP) is a new technology for cell transfection that offers improved gene delivery and cell viability compared to conventional bulk electroporation (BEP). NEP uses an asymmetric electric field to induce asymmetric membrane poration of cells laid on a porous substrate. This study systematically investigates the transfection window of NEP for different sized molecules and shows that small molecules can be delivered with minimal impact on the non-transfection membrane, while larger molecules require higher voltages but can result in lower cell viability. Numerical analysis of transmembrane potential allows for prediction of membrane damage and cargo delivery in NEP, providing a comprehensive understanding of the process.
Article
Polymer Science
Beste Cagdas Tunali, Omer Akturk, Durukan Sahingoz, Mustafa Turk, Ayten Celebi Keskin
Summary: In this study, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized using the pulsed laser ablation in liquid (PLAL) technique, and then combined with Bombyx mori silk fibroin (SF) to form nanocomposites (PEI-SF-AuNPs). The biocompatibility of PEI-AuNPs and PEI-SF-AuNPs in breast cancer cells MCF-7 and MDA-MB231 were tested, and it was found that PEI-AuNP1 showed the least cytotoxicity. Therefore, PEI-AuNP1 nanocomposites can be suggested as siRNA carriers in gene-based breast cancer therapies.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Multidisciplinary Sciences
Krishnapriya Syama, Zygmunt J. Jakubek, Sam Chen, Josh Zaifman, Yuen Yi C. Tam, Shan Zou
Summary: This study evaluated the toxicity of six lipid-based formulations on cells and found that anionic, cationic, and neutral lipid formulations were non-toxic. These findings suggest that these lipid-based formulations have potential as drug carriers.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Viriato M'Bana, Aparajita Lahree, Sofia Marques, Ksenija Slavic, Maria M. Mota
Summary: The UIS4 protein interacts with host cell actin, suppressing filamentous actin formation to avoid parasite elimination. Host cell actin dynamics increase around UIS4-deficient parasites, leading to parasite elimination.
Article
Microbiology
Somayeh Azami, Ehsan Arefian, Nasim Kashef
Summary: The study showed that the postbiotics of Lactobacillus casei could effectively reduce the virulence and biofilm formation of Pseudomonas aeruginosa, suggesting a promising natural alternative for anti-virulence treatments without cytotoxic effects on cells.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Anousha Khamsavi, Yaser Abdi, Zahra Fekrirad, Ehsan Arefian
Summary: A glioblastoma cancer cell biosensor based on mono-layer graphene/(n-type)Si Schottky junction is proposed in this study. The sensor can distinguish different glioblastoma cancer cell lines and differentiate them from healthy cells, providing a new avenue for cancer cell detection and differentiation.
IEEE SENSORS JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Mohammad Reza Kalhori, Masoud Soleimani, Ehsan Arefian, Ali Mohammad Alizadeh, Kamran Mansouri, Javier Echeverria
Summary: miR-1290 is dysregulated in many cancers and plays a crucial role in their development, making it a potential diagnostic and therapeutic target. This systematic review study also discussed how miR-1290 alters the expression of genes related to cancer development and resistance to chemo-radiation, as well as the regulatory effect of natural products on miR-1290 expression and its interaction with lncRNAs.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Cell Biology
Zeinab Dehghani-Ghobadi, Shahrzad Sheikh Hasani, Ehsan Arefian, Ghamartaj Hossein
Summary: This study investigates the association of Wnt5A with the TGF-beta 1/Smad2/3 and Hippo-YAP1/TAZ-TEAD pathways in epithelial ovarian cancer, and suggests that Wnt5A may be a critical mediator of EMT-associated pathways.
Article
Biochemistry & Molecular Biology
Faezeh Sahebdel, Azita Parvaneh Tafreshi, Ehsan Arefian, Eleni Roussa, Samad Nadri, Bahman Zeynali
Summary: The study found that trabecular meshwork-derived mesenchymal stem cells (TM-MSCs) have higher neurogenic differentiation potential compared to adipose tissue-derived mesenchymal stem cells (AD-MSCs) and bone marrow-derived stem cells (BMSCs). The activation of the Wnt/beta-catenin pathway directs the neurally induced TM-MSCs towards a dopaminergic fate.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Shokoofeh Ghaemi, Zahra Fekrirad, Nina Zamani, Rana Rahmani, Ehsan Arefian
Summary: The treatment of brain tumors, especially glioblastoma multiforme (GBM), remains challenging. Both intra- and inter-tumor heterogeneity contribute to resistance and tumor recurrence. However, the discovery of non-coding RNAs, such as microRNAs and long non-coding RNAs, has shed light on their potential to regulate tumor growth and improve therapeutic outcomes. Combining these non-coding RNAs with chemotherapeutic compounds and suicide genes offers an innovative therapeutic approach for managing GBM.
JOURNAL OF DRUG TARGETING
(2022)
Article
Reproductive Biology
Atieh Hajarizadeh, Akram Eidi, Ehsan Arefian, Eva Tvrda, Abdollah Mohammadi-Sangcheshmeh
Summary: Aflatoxin B1 (AFB1) negatively affects the development of sheep oocytes and embryonic development, leading to decreased meiotic and cytoplasmic maturation, increased intracellular reactive oxygen species (ROS) production, decreased mitochondrial membrane potential, and altered gene expression related to apoptosis and DNA methylation.
Article
Biochemistry & Molecular Biology
Morteza Ahmadzadeh-Darinsoo, Mojtaba Ahmadzadeh-Darinsoo, Shahsanam Abbasi, Ehsan Arefian, Claude Bernard, Azita Parvaneh Tafreshi
Summary: This study examined the effects of the specific GSK3 beta inhibitor 7-BIO on cell survival and expression of miR-29a-3p and miR-34a-5p in neurotoxin MPP+ treated cells. The findings showed that 7-BIO increased cell survival and regulated the expressions of miR-29a-3p and miR-34a-5p.
NON-CODING RNA RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Behnaz Bakhshandeh, Nika Ranjbar, Ardeshir Abbasi, Elahe Amiri, Ali Abedi, Mohammad-Reza Mehrabi, Zahra Dehghani, Cristian Pablo Pennisi
Summary: Tissue engineering is a cutting-edge discipline that combines cells, scaffolds, and induction factors to regenerate and repair damaged tissue. Understanding the effects of biochemical and biophysical factors on cell fate is crucial for improving the efficiency of tissue engineering approaches. Biophysical signals provide a robust set of stimuli to manipulate cell signaling pathways during tissue formation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Engineering, Biomedical
Zohreh Jahanafrooz, Behnaz Bakhshandeh, Seyyed Behnam Abdollahi, Ehsan Seyedjafari
Summary: The human amniotic membrane, or amnion, is a potential tissue graft with wide clinical applications. Its collagen-based chemical composition and mechanical properties make it suitable for tissue engineering. The presence of cells and growth factors, along with its easy processing and storage, anti-inflammatory, antioxidant, antibacterial, and non-immunogenic properties, make amnion an ideal choice in regenerative medicine. From simple skin or cornea repair to complex regeneration of cartilage, muscle, tendon, and heart, amnion has evolved as a high-tech material. It also has anticancer properties and can be used for drug and cell delivery.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Immunology
Mona Sadat Mirtaleb, Reza Falak, Jalal Heshmatnia, Behnaz Bakhshandeh, Ramezan Ali Taheri, Hoorieh Soleimanjahi, Reza Zolfaghari Emameh
Summary: The global spread of SARS-CoV-2 has led to the development of novel vaccine platforms for prolonged immunity against respiratory viral infections. Despite concerns about mRNA-based vaccines, they have been effective in reducing the severity of COVID-19 and meeting global demand.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Andrology
Mohammad Mehdi Naghizadeh, Behnaz Bakhshandeh, Farshid Noorbakhsh, Marjan Yaghmaie, Ali Masoudi-Nejad
Summary: The traditional approach to constructing the miRNA-mRNA network involves differential expression and direct targeting of mRNA by miRNA. However, this approach may lead to the loss of information and challenges in direct targeting. To overcome these problems, we analyzed rewiring networks and constructed miRNA-mRNA expression bipartite networks for normal and prostate cancer tissue. We identified significant changes in regression coefficients between normal and cancer states, defined rewired edges and nodes, and analyzed the enriched network. Our findings highlighted the centrality of specific mRNAs and miRNAs, as well as the enrichment of molecular functions such as SMAD and beta-catenin binding.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2023)
Article
Multidisciplinary Sciences
Behnaz Bakhshandeh, Zohreh Jahanafrooz, Shiva Allahdadi, Shiva Daryani, Zahra Dehghani, Mahya Sadeghi, Mir Sepehr Pedram, Mohammad Mehdi Dehghan
Summary: Induced pluripotent stem cells (iPSCs) can secrete microvesicles (MVs) that have the potential for skin healing and rejuvenation. This study evaluated the possibility, safety, and effectiveness of using iPSCs-derived MVs for skin tissue engineering and regeneration. The results showed that MVs can promote skin cell growth and repair, and have beneficial effects on skin regeneration in rat burn wound models.
SCIENTIFIC REPORTS
(2023)
Review
Oncology
Behnaz Bakhshandeh, Shokufeh Ghasemian Sorboni, Nika Ranjbar, Roham Deyhimfar, Maryam Sadat Abtahi, Mehrnaz Izady, Navid Kazemi, Atefeh Noori, Cristian Pablo Pennisi
Summary: Stem cells are influenced by biochemical and biophysical cues from the extracellular matrix and neighboring cells. Biomechanical forces play a crucial role in regulating stem cell behavior and fate through mechanotransduction. Recent studies have highlighted the importance of biomechanical signals in tissue regeneration and shown the existence of precise control mechanisms in stem cell niches.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Zohreh Jahanafrooz, Behnaz Bakhshandeh, Erfan Shirzadi
Summary: The aim of this study was to determine the transfection yields of two commercially available transfection reagents (Lipofectamine 2000 and PAMAM G5) in breast cancer cells and non-cancer cells. The results showed that Lipofectamine 2000 had significantly better efficiency for short RNA transfection in both cell types compared to PAMAM G5. This research provides a route for comprehensive epigenetic modification of cancer cells and depicts an approach to efficient drug delivery.
ADVANCED PHARMACEUTICAL BULLETIN
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
