Journal
MOLECULAR AND CELLULAR PROBES
Volume 29, Issue 6, Pages 511-513Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2015.09.001
Keywords
RT-qPCR; MERS; Coronavirus; TaqMan (R)-MGB
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Funding
- Defense Threat Reduction Agency (DTRA) [CB3901]
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Here we designed and tested two highly specific quantitative TaqMan (R)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection CLOD) of 0.005 plaque-forming units/PCR (pfu/PCR). Published by Elsevier Ltd.
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