Article
Biochemistry & Molecular Biology
Hemangi Ranade, Priya Paliwal, Anis Ahmad Chaudhary, Sakshi Piplani, Hassan Ahmed Rudayni, Mohammed Al-Zharani, Ravi Ranjan Niraj, Manali Datta
Summary: This study assessed the interaction between protease and inhibitor to design an electrochemical diagnostic module for epithelial ovarian cancer. The predictions of protease candidates were confirmed experimentally, and selectivity and sensitivity of their electrochemical interactions were evaluated. The results validate the utility of dry-lab integration in the wet-lab technique for the design of electrochemical diagnostics for epithelial ovarian cancer.
Article
Oncology
Yongsheng Jiang, Lijie Han, Meilin Xue, Ting Wang, Youwei Zhu, Cheng Xiong, Minmin Shi, Hongzhe Li, Wangxi Hai, Yanmiao Huo, Baiyong Shen, Lingxi Jiang, Hao Chen
Summary: This study found that autophagy and glycolysis play important roles in tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). Cystatin B (CSTB) acts as a key player in orchestrating these processes to ensure energy supply in PDAC cells.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Abdullah Md Sheikh, Yasuko Wada, Shatera Tabassum, Satoshi Inagaki, Shingo Mitaki, Shozo Yano, Atsushi Nagai
Summary: The study found that Cystatin C aggregation in cerebral amyloid angiopathy may alter the protein's activity, leading to cellular degeneration and toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Zijun Zhang, Fenghuang Zhan
Summary: Type 2 cystatins are a group of small secreted protease inhibitors that play important roles in regulating cellular processes related to immune response and tumor progression. This review provides an overview of the biological similarities and differences of type 2 cystatins, their regulatory effect on human immune responses, and their roles in tumor progression, immune evasion, and metastasis.
Review
Pharmacology & Pharmacy
Upinder Kaur, Sankha Shubhra Chakrabarti, Bisweswar Ojha, Bhairav Kumar Pathak, Amit Singh, Luciano Saso, Sasanka Chakrabarti
Summary: The spread of SARS-CoV-2 worldwide has led to devastating consequences, with the lack of definitive therapy necessitating symptomatic management as the standard of care. Repurposing existing drugs, including antiviral drugs, is being attempted to combat the COVID-19 pandemic, with a potential focus on targeting host proteases involved in the pathogenesis of SARS-CoV-2.
CURRENT DRUG TARGETS
(2021)
Article
Neurosciences
Yixing Wu, Paige Mumford, Suzanna Noy, Karen Cleverley, Alicja Mrzyglod, Dinghao Luo, Floris van Dalen, Martijn Verdoes, Elizabeth M. C. Fisher, Frances K. Wiseman
Summary: Cathepsin B, a cysteine protease, is involved in various aspects of Alzheimer's disease. People with Down syndrome, who have an extra copy of chromosome 21, also have an additional copy of an endogenous inhibitor of this enzyme. The activity of cathepsin B is found to be decreased in the brain of individuals with both Down syndrome and Alzheimer's disease, while the abundance of the inhibitor cystatin B is significantly increased in their brains. Understanding the biology of these proteins in people with Down syndrome and Alzheimer's disease can lead to better therapeutics and insights into disease pathogenesis.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Veterinary Sciences
Ahlame Saidi, Pauline Gaboriaud, Anne-Christine Lalmanach, Lise Vanderlynden, Aurelie Fessard, Pauline Vettori, Genevieve Fort, Rodrigo Guabiraba, Catherine Schouler, Fabrice Laurent, Edouard Guitton, Fabien Lecaille, Francoise I. Bussiere, Gilles Lalmanach
Summary: During coccidiosis infection, host cysteine cathepsin activity increases, potentially contributing to the alteration of intestinal proteolytic balance. Furthermore, cysteine cathepsins may be involved in inflammation during coccidiosis by proteolytically inactivating up-regulated avian beta-defensins.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ana Mitrovic, Emanuela Senjor, Marko Jukic, Lara Bolcina, Mateja Prunk, Matic Proj, Milica Perisic Nanut, Stanislav Gobec, Janko Kos
Summary: A new potent inhibitor of cathepsin V has been identified, which significantly affects cell proliferation, elastin degradation, and immune cell cytotoxicity, suggesting its potential application in cancer therapy.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Virology
Kamal Shokeen, Shambhavi Pandey, Manisha Shah, Sachin Kumar
Summary: This study analyzed the interaction between the S protein of SARS-CoV-2 and different host proteases using molecular docking and molecular dynamics simulation. It found that mutations in the sensitive cleavage site of the S protein can disrupt the binding of cellular proteases. This mutation study may help generate an attenuated SARS-CoV-2 and targeting host proteases with inhibitors could be a practical approach to stop the cellular spread of the virus and develop antiviral treatments.
Article
Nutrition & Dietetics
Juliana A. S. Leite, Carlos A. Montoya, Simon M. Loveday, Evelyne Maes, Jane A. Mullaney, Warren C. McNabb, Nicole C. Roy
Summary: The study found that there are differences in non-specific protease activity in whey and casein fractions among different ruminant milk species. Specific thermal treatments could be utilized to retain better protease activity in all ruminant milk species.
FRONTIERS IN NUTRITION
(2021)
Review
Biochemistry & Molecular Biology
Adam Miszta, Dana Huskens, Demy Donkervoort, Molly J. M. Roberts, Alisa S. Wolberg, Bas de Laat
Summary: Fibrinolysis is a crucial process in wound healing, with plasmin playing a central role in dissolving clots. Recent advancements have enabled the quantification of plasmin generation (PG) and inhibition kinetics during fibrinolysis. Understanding plasmin kinetics aids in identifying fibrinolytic dysfunction and investigating the link between abnormal fibrin dissolution and disease pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Debora do Carmo Linhares, Fernanda Faria, Roberto Tadashi Kodama, Adriane Michele Xavier Prado Amorim, Fernanda Calheta Vieira Portaro, Dilza Trevisan-Silva, Karla Fernanda Ferraz, Ana Marisa Chudzinski-Tavassi
Summary: Cathepsin L (CatL) is a critical lysosomal cysteine protease in human diseases, including cancer and coronary artery diseases. A potent inhibitor of CatL was biochemically characterized from the leech Cystatin-Hv, suggesting potential therapeutic applications in future studies involving cellular and in vivo models.
Article
Pharmacology & Pharmacy
So-Hyeon Park, Jun-Hyuck Lee, Seong-Bin Yang, Dong-Nyeong Lee, Tae-Bong Kang, Jooho Park
Summary: A novel peptide and drug conjugate (PDC) called RR-BA was developed using a cathepsin-B-specific peptide (RR) and bile acid (BA), which could self-assemble in an aqueous solution to form stable nanoparticles. The nano-sized RR-BA conjugate exhibited significant inhibitory effects on cathepsin B and showed anticancer effects against mouse colorectal cancer cells (CT26). In vivo experiments in CT26 tumor-bearing mice also confirmed its therapeutic effect and low toxicity. Therefore, the RR-BA conjugate holds promise as an effective anticancer drug candidate for targeting cathepsin B in cancer therapy.
Review
Oncology
Gilles Lalmanach, Mariana Kasabova-Arjomand, Fabien Lecaille, Ahlame Saidi
Summary: Cystatin M/E, a low molecular mass protein, exhibits both tumor-suppressing and tumor-promoting effects in various cancers. Understanding the regulatory mechanisms of Cystatin M/E and its expression and activity in tumorigenesis is crucial in maintaining proteolytic balance and addressing its imbalance in cancer.
Review
Biochemistry & Molecular Biology
Fabien Lecaille, Thibault Chazeirat, Ahlame Saidi, Gilles Lalmanach
Summary: This review discusses the role of human cysteine cathepsin V in various biological and pathophysiological processes. It provides an overview of the molecular aspects, including genomic organization, protein structure, and substrate specificity. The dysregulation of cathepsin V expression and activity is related to the progression of malignancies and vascular disorders. The article highlights the potential of cathepsin V as a therapeutic target.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)