Article
Multidisciplinary Sciences
Nikolay Zolotarev, Marc Bayer, Rudolf Grosschedl
Summary: The continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Carolin Goebel, Shweta Godbole, Melanie Schoof, Doerthe Holdhof, Catena Kresbach, Carolin Loose, Julia Neumann, Ulrich Schueller
Summary: This study demonstrates the important role of SMARCA4 deficiency in the development of Group 3 medulloblastoma and provides insights into the deregulated gene expression involved in SMARCA4/MYC-driven tumorigenesis.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Oncology
Takaaki Yoshikawa, Akihisa Fukuda, Mayuki Omatsu, Mio Namikawa, Makoto Sono, Yuichi Fukunaga, Tomonori Masuda, Osamu Araki, Munemasa Nagao, Satoshi Ogawa, Kenji Masuo, Norihiro Goto, Yukiko Hiramatsu, Yu Muta, Motoyuki Tsuda, Takahisa Maruno, Yuki Nakanishi, Kenji Kawada, Shigeo Takaishi, Hiroshi Seno
Summary: Brg1 is crucial for intestinal TSCs in mice by inhibiting apoptosis and is critical for cell survival and stem cell features in human CRC cells, representing a new therapeutic target for human CRC.
JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Donghui Zhang, Yanmei Zhu, Yanmin Ju, Hongyong Zhang, Xiaopeng Zou, Shangrong She, Danping Zhu, Yiting Guan
Summary: Dramatic changes in chromatin structure occur during cellular senescence, affecting genome accessibility and transcription. This study investigates the redistribution of accessible chromatin regions during senescence and identifies the transcription factor TEAD4 as a key regulator of chromatin state and SASP gene transcription.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Reproductive Biology
Atefeh Abedini, David A. Landry, Angus D. Macaulay, Het Vaishnev, Ashna Parbhakar, Dalia Ibrahim, Reza Salehi, Vincent Maranda, Elizabeth Macdonald, Barbara C. Vanderhyden
Summary: The study investigates the role of BRG1 in female fertility using mutant mice. Depletion of Brg1 in granulosa cells causes sterility, while depletion in oocytes results in subfertility. These findings highlight the importance of BRG1 in regulating granulosa and oocyte functions during follicle growth and its necessity for oocyte developmental competence.
BIOLOGY OF REPRODUCTION
(2023)
Article
Immunology
Dominik Schmiedel, Hadas Hezroni, Amit Hamburg, Ziv Shulman
Summary: The research reveals that Brg1 plays a critical role in B cell proliferation and germinal center formation through selective interactions with enhancers. Recruitment of Brg1 to enhancers increases chromatin accessibility, leading to the regulation of gene expression associated with cell cycle and supporting B cell proliferation and the formation of germinal center reactions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Bongwoo Kim, Yi Luo, Xiaoming Zhan, Zilai Zhang, Xuanming Shi, Jiaqing Yi, Zhenyu Xuan, Jiang Wu
Summary: The core subunit BRG1 of the SWI/SNF-like BAF ATP-dependent chromatin remodeling complexes regulates neuronal activity-induced enhancers by affecting enhancer activities and inducibility. BRG1 phosphorylation induced by neuronal stimulations modulates its interaction with transcription co-factors, potentially influencing transcription outcomes. Non-phosphorylatable BRG1 fails to efficiently induce activity-dependent genes, while phosphomimic BRG1 increases enhancer activity and inducibility.
Article
Cell Biology
Ming Kong, Yuwen Zhu, Jing Shao, Zhiwen Fan, Yong Xu
Summary: BRG1 deficiency in mice results in reduced processing and nuclear accumulation of SREBP in the liver, affecting lipid metabolism. BRG1 plays a crucial role in regulating SREBP by activating SCAP transcription, ultimately influencing SREBP target gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jinshu Xu, Xianxiao Zhou, Ting Zhang, Bin Zhang, Pin-Xian Xu
Summary: This study investigates the role of chromatin remodeling factor Smarca4 in kidney development and disease. The results demonstrate that deficiency of Smarca4 leads to tubular defects, medulla shortening, altered cell characteristics, and dysregulated gene expression, which eventually result in fibrosis. Furthermore, Smarca4 loss upregulates the oncogene Pttg1 and promotes hyperproliferation of Wnt4(Cre) labeled cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Mallory Wiggans, Shu Jun Zhu, Alyssa M. Molinaro, Bret J. Pearson
Summary: Our study provides functional insights into how the BAF complex contributes to cell fate decisions in planarian ASCs in vivo, showing that it promotes chromatin accessibility and facilitates transcription at target loci, enabling access to genes required for the generation of certain cell lineages. However, BAF complex knockdowns do not affect the formation of endodermal lineages.
Article
Biology
Nicholas Dietrich, Kevin Trotter, James M. Ward, Trevor K. Archer
Summary: The study identifies BCL7 proteins as important members of the SWI/SNF complex, working with BRG1 to regulate gene expression by remodeling chromatin structure. The interaction between BCL7 and BRG1 is mediated by the HSA domain, highlighting its role in forming a functional SWI/SNF complex. The correct formation of the complex is crucial for driving critical biological functions.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biochemistry & Molecular Biology
Aditi Singh, Xyrus X. Maurer-Alcala, Therese Solberg, Lilia Haeussermann, Silvan Gisler, Michael Ignarski, Estienne C. Swart, Mariusz Nowacki
Summary: Recruiting the chromatin remodeler ISWI1 facilitates IES recognition and precise excision in Paramecium, ensuring efficient DNA elimination.
Review
Biochemistry & Molecular Biology
Sarah Larrigan, Sujay Shah, Alex Fernandes, Pierre Mattar
Summary: During brain development, the genome is repeatedly reconfigured to aid neuronal and glial differentiation, with a range of chromatin remodeling complexes facilitating the process. The non-redundancy of these complexes suggests a need for a variety of remodelers with different specificities and activities in neurodevelopment. The nucleosome remodeling and deacetylase (NuRD) complex plays a key role in the biochemistry, genetics, and functions of neural progenitors and neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Beatriz Monterde, Ignacio Varela
Summary: SWI/SNF gene alterations are common in lung cancer and are associated with prognosis. These alterations can be explored for potential therapeutic strategies to improve the treatment of lung cancer patients.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Eunhye Lee, Chanshin Kang, Pasi Purhonen, Hans Hebert, Karim Bouazoune, Sungchul Hohng, Ji-Joon Song
Summary: CHD7 is a crucial chromatin remodeler, and its N-CRD region is essential for its ATPase stimulation and nucleosome remodeling activity. Mutations in this region can lead to defects in CHD7 function, affecting remodeling activity.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Cell Biology
Julia R. Wilkerson, Joseph P. Albanesi, Kimberly M. Huber
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2018)
Article
Neurosciences
Sonal Goswami, Sheridan Cavalier, Vinay Sridhar, Kimberly M. Huber, Jay R. Gibson
NEUROBIOLOGY OF DISEASE
(2019)
Article
Biochemistry & Molecular Biology
Botao Liu, Gemma Molinaro, Huan Shu, Emily E. Stackpole, Kimberly M. Huber, Joel D. Richter
NUCLEIC ACIDS RESEARCH
(2019)
Article
Neurosciences
Darya Gonzalez, Madison Tomasek, Seth Hays, Vinay Sridhar, Simon Ammanuel, Chia-wei Chang, Karen Pawlowski, Kimberly M. Huber, Jay R. Gibson
JOURNAL OF NEUROSCIENCE
(2019)
Article
Cell Biology
Patrick K. McCamphill, Laura J. Stoppel, Rebecca K. Senter, Michael C. Lewis, Arnold J. Heynen, David C. Stoppel, Vinay Sridhar, Katie A. Collins, Xi Shi, Jen Q. Pan, Jon Madison, Jeffrey R. Cottrell, Kimberly M. Huber, Edward M. Scolnick, Edward B. Holson, Florence F. Wagner, Mark F. Bear
SCIENCE TRANSLATIONAL MEDICINE
(2020)
Article
Cell Biology
Sneha Shah, Gemma Molinaro, Botao Liu, Ruijia Wang, Kimberly M. Huber, Joel D. Richter
Article
Genetics & Heredity
Annie Hien, Gemma Molinaro, Botao Liu, Kimberly M. Huber, Joel D. Richter
Article
Multidisciplinary Sciences
Seung-Kyoon Kim, Xihui Liu, Jongmin Park, Dahun Um, Gokhul Kilaru, Cheng-Ming Chiang, Mingon Kang, Kimberly M. Huber, Keunsoo Kang, Tae-Kyung Kim
Summary: This study demonstrates the different roles of three major BET family proteins in transcription, and their sensitivity to the bromodomain inhibitor JQ1. In an FXS model, BRD2/3 and BRD4 show opposite changes in expression and chromatin binding, correlating with transcriptional dysregulation. Acute inhibition of CBP/p300 HAT activity can restore the altered binding patterns of BRD2 and BRD4, and rescue memory impairment in FXS.
Article
Psychiatry
Tori L. Schaefer, Amy A. Ashworth, Durgesh Tiwari, Madison P. Tomasek, Emma V. Parkins, Angela R. White, Andrew Snider, Matthew H. Davenport, Lindsay M. Grainger, Robert A. Becker, Chandler K. Robinson, Rishav Mukherjee, Michael T. Williams, Jay R. Gibson, Kimberly M. Huber, Christina Gross, Craig A. Erickson
Summary: Fragile X syndrome is the most common cause of inherited intellectual disability, resulting from the loss of Fragile X Protein. While modulation of the GABAergic system has been suggested as a treatment strategy, clinical trials using broad spectrum GABA receptor-specific agonists have not shown broad improvements in FXS phenotypes. The experimental drug BAER-101, a selective GABA(A) alpha 2/alpha 3 agonist, has shown promising results in reducing hyperexcitability in cortical circuits and improving certain phenotypes in Fmr1 KO mice.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Biology
Zhe Zhang, Jay R. Gibson, Kimberly M. Huber
Summary: Reduced interhemispheric connectivity is correlated with severity of ASD behaviors, and Fmr1 plays a cell-autonomous role in promoting maturation of callosal synapses. Deletion of Fmr1 results in weakened callosal synaptic function, which can be normalized by sensory deprivation. Fmr1 has an input-specific role in regulating synaptic connectivity within local and callosal neocortical circuits, with implications for underconnectivity observed in FXS patients.
Editorial Material
Neurosciences
Kimberly M. Huber, Pierre Paoletti, P. Jesper Sjostrom
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Brianne Dentel, Lidiette Angeles-Perez, Chongyu Ren, Vikram Jakkamsetti, Andrew J. Holley, Daniel Caballero, Emily Oh, Jay Gibson, Juan M. Pascual, Kimberly M. Huber, Benjamin P. Tu, Peter T. Tsai
Summary: In this study, a mouse model was used to investigate the mechanisms underlying epilepsy associated with the GATOR1 signaling negative regulator NPRL2. Mutant mice showed increased mTORC1 signaling, spontaneous seizures, and abnormal synaptic function. Proteomic and metabolomic studies revealed alterations in epilepsy-implicated proteins and metabolic pathways, providing potential therapeutic targets for epilepsy.
Article
Neurosciences
Andrew J. Holley, Aleya Shedd, Anna Boggs, Jonathan Lovelace, Craig Erickson, Christina Gross, Miranda Jankovic, Khaleel Razak, Kimberly Huber, Jay R. Gibson
Summary: The study found that Fmr1 deletion in brainstem neurons is necessary for certain aspects of the decreased phase-locking phenotype in the Fmr1 KO mouse, but not necessary for the increase in non-phase-locked power induced by sound. Reinstating FMRP in brainstem neurons can rescue specific EEG phenotypes, but not all.
NEUROBIOLOGY OF DISEASE
(2022)
Article
Neurosciences
Barbara Barylko, Per Niklas Hedde, Clinton A. Taylor, Derk D. Binns, Yu-Kai Huang, Gemma Molinaro, Kimberly M. Huber, David M. Jameson, Joseph P. Albanesi
Summary: CaMKv, a pseudokinase expressed predominantly in brain and neural tissue, may play a role in synaptic strengthening by promoting the stabilization and enrichment of dendritic spines. This study confirms that CaMKv is palmitoylated on Cys5 and interacts with another synaptic plasticity regulator, Arc/Arg3.1.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Barbara Barylko, Julia R. Wilkerson, Sheridan H. Cavalier, Derk D. Binns, Nicholas G. James, David M. Jameson, Kimberly M. Huber, Joseph P. Albanesi