Journal
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
Volume 41, Issue 7, Pages 867-873Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.ijom.2011.12.032
Keywords
mandibular distraction osteogenesis; immunohistochemistry; PCNA; BMP4
Categories
Funding
- AO-ASIF Foundation (Berne, Switzerland)
- Hanson Foundation (Boston, MA)
- CMF (West Chester, PA)
- Massachusetts General Hospital Oral and Maxillofacial Surgery Education and Research Fund (Boston, MA)
- Harvard Medical School Office of Enrichment
Ask authors/readers for more resources
This study characterized sequential molecular and cellular events in the porcine mandibular distraction osteogenesis (DO) wound. Nineteen Yucatan minipigs were divided into three treatment groups: Group A, unilateral mandibular distraction with 0 day latency, 1 mm/day rate for 12 days, 24 days fixation (n = 16); Group B, acute lengthening 12 mm (n = 2); Group C, sham control (n = 1). Group A was further divided by death date: mid-DO (n = 5), end-DO (n = 4), mid-fixation (n = 5) and end-fixation (n = 2). Groups B and C were killed on postoperative day 36, corresponding to end-fixation. Specimens were stained for proliferating cell nuclear antigen (PCNA) and bone morphogenetic protein-4 (BMP4). Cellular proliferation (PCNA) was assessed quantitatively and BMP4 staining was assessed on a semi-quantitative scale. Progenitor cell proliferation was greatest during mid-DO and decreased from end-DO through end-fixation. Proliferation in the acute lengthening group was elevated relative to sham control and comparable to end-DO. BMP4 staining intensity (localized to the periosteal cambium layer) was greatest during mid- and end-DO, decreased at mid-fixation and was undetectable at end-fixation. Progenitor cell proliferation and BMP4 expression are greatest during mid-DO and decrease progressively thereafter. At the time of death of the acute lengthening group, only increased cell proliferation was demonstrated.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available