Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 199, Issue 1-2, Pages 1-4Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2015.02.003
Keywords
mRNA translation; RNA-binding proteins; Trypanosoma brucei
Categories
Funding
- National Institutes of Health [AI108290]
- Wellcome Trust [085956]
- Royal Society Joint Project [2008/R2]
- Spanish Ministry of Science and Technology [TIN2009-13950/TIN2012-38805, BFU 2009-07510]
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Trypanosomes are early-branched eukaryotes that show an unusual dependence on post-transcriptional mechanisms to regulate gene expression. RNA-binding proteins are crucial in controlling mRNA fate in these organisms, but their RNA substrates remain largely unknown. Here we have analyzed on a global scale the mRNAs associated with the Trypanosoma brucei RNA-binding protein DRBD3/PTB1, by capturing ribonucleoprotein complexes using UV cross-linking and subsequent immunoprecipitation. DRBD3/PTB1 associates with many transcripts encoding ribosomal proteins and translation factors. Consequently, silencing of DRBD3/PTB1 expression altered the protein synthesis rate. DRBD3/PTB1 also binds to mRNAs encoding the enzymes required to obtain energy through the oxidation of proline to succinate. We hypothesize that DRBD3/PTB1 is a key player in RNA regulon-based gene control influencing protein synthesis in trypanosomes. (C) 2015 Elsevier B.V. All rights reserved.
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