Article
Multidisciplinary Sciences
Keijiro Ohshima, Tomoko Ohshima, Karen Meyer, Eisuke Takai, Shunsuke Yoshizawa, Kentaro Shiraki, Nobuko Maeda
Summary: There are differences in saliva protein composition between the two strains of mice, BALB/cA and C3H/HeN, and this may contribute to their varying sensitivity to caries. Proteomic analysis revealed that C3H/HeN mice have a higher quantity of high-affinity saliva proteins and higher CAVI activity compared to BALB/cA mice, suggesting a relationship between these factors and caries susceptibility.
Article
Biology
Zuzana Kovacova, Ingrid Hodorova
Summary: This study focused on detecting CAIX and survivin in colorectal adenocarcinoma cells in the Slovakian population. The results showed that the expression of these proteins did not significantly correlate with clinicopathological parameters, suggesting they could be useful as potential tumor diagnostic markers. CAIX and survivin may serve as negative independent prognostic markers for colorectal cancer.
Article
Biochemistry & Molecular Biology
Mingli Hsieh, Pei-Ju Huang, Pei-Yu Chou, Shih-Wei Wang, Hsi-Chi Lu, Wei-Wen Su, Yuan-Chiang Chung, Min-Huan Wu
Summary: Research has shown that CAVIII is overexpressed in colorectal cancer tissues and more aggressive cancer cells. It may facilitate the release of VEGF by inhibiting miRNA16-5p. The CAVIII/miR-16-5p signaling pathway appears to function as a metastasis suppressor in CRC.
Article
Oncology
Hsien-Cheng Huang, Bei-Hao Shiu, Yasser Nassef, Chi-Chou Huang, Ying-Erh Chou, Wen-Chien Ting, Lun-Ching Chang, Jian-Cheng Lin, Li-Kai Hsiao, Shun-Fa Yang, Shih-Chi Su
Summary: The study found that CA9 gene polymorphisms were not significantly associated with the occurrence of CRC, but the association analysis indicated that the rs2071676 genotype had a specific impact on the development of colon cancer.
Article
Chemistry, Analytical
Wen Li, Baofang Zhang, Zilin Chen
Summary: The study developed an EMMA method which combines TDLFP and rapid polarity switching technology to achieve rapid mixing of different reactants. By optimizing the enzyme reaction and separation conditions, the effectiveness of this method for screening carbonic anhydrase IX inhibitors was successfully validated.
Article
Biochemistry & Molecular Biology
Giulia Nannini, Viviana De Luca, Chiara D'Ambrosio, Andrea Scaloni, Antonio Taddei, Maria Novella Ringressi, Fabio Cianchi, Fabio Staderini, Clemente Capasso, Amedeo Amedei, Claudiu T. Supuran
Summary: This study investigated the expression of carbonic anhydrase isoforms in tumour-infiltrating lymphocytes (TILs) in colorectal cancer. The results showed that T lymphocytes isolated from healthy surrounding mucosa had higher carbonic anhydrase activity compared to those in tumour and peripheral blood in the same patients, suggesting a dysregulation of the local immune response and a loss of effective anticancer mechanisms mediated by carbonic anhydrase in CRC tissues.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Afsana Mahim, David H. Petering
Summary: This study investigated the impact of glutathione (GSH) on zinc (Zn) trafficking from proteome to apo-carbonic anhydrase. The results showed that depletion of GSH stimulated cellular Zn accumulation and the proteome and metallothionein (MT) served as functional sources of Zn for generating Zn-proteins. The study also supported the hypothesis that enhanced transport of Zn into cells drives the conversion of apo-Zn-proteins to Zn-proteins.
Article
Cell Biology
Monika Wolny, Svitlana Rozanova, Cornelius Knabbe, Kathy Pfeiffer, Katalin Barkovits, Katrin Marcus, Ingvild Birschmann
Summary: This study aims to further understand the role of platelets in COVID-19 progression and identify predictive biomarkers for disease outcomes. Comparative proteome analysis of platelets from critically ill COVID-19 patients showed significant changes in proteins associated with protein folding and an increase in proteins with isomerase activity, which influenced platelet activity via non-genomic properties of the glucocorticoid receptor and the nuclear factor κB.
Review
Biochemistry & Molecular Biology
Mirfath Sultana Mesbahuddin, Aravindhan Ganesan, Subha Kalyaanamoorthy
Summary: Researchers are bioengineering carbonic anhydrases (CAs) with adaptabilities to extreme conditions, using modern technologies to predict stable variants. Through protein engineering and molecular dynamics simulations, more stable CA variants have been obtained under extreme conditions, providing a new avenue for CO2 capture and sequestration.
PROTEIN ENGINEERING DESIGN & SELECTION
(2021)
Article
Entomology
Tomas Erban, Elena Shcherbachenko, Pavel Talacko, Karel Harant
Summary: Despite extensive studies on honey bee honey, there is limited information on bumblebee honey. This study compared the proteomes of Bombus terrestris honey and Apis mellifera honey, and identified substantial differences, particularly in protein composition, between the two types of honey. These differences may explain variations in their eusocial organization.
Article
Cell Biology
Ya Peng, Zhaoyu Zhang, Ailing Zhang, Changhong Liu, Yingnan Sun, Zixuan Peng, Yang Liu
Summary: Annexin A4 (ANXA4) is highly expressed and translocated to the membrane and cytoplasm in colorectal carcinoma (CRC). CAMK2 gamma is important for high ANXA4 expression, while CA1 promotes ANXA4 aggregation on the cell membrane. Increased Ca2+ concentration attenuates SUMO modification of cytoplasmic ANXA4, leading to ANXA4 stabilization and promotion of CRC tumorigenesis and EMT.
Article
Pharmacology & Pharmacy
Siao-Syun Guan, Cheng-Tien Wu, Tse-Zung Liao, Kun-Liang Lin, Cheng-Liang Peng, Ying-Hsia Shih, Mao-Feng Weng, Chun-Tang Chen, Chung-Hsin Yeh, Ying-Chieh Wang, Shing-Hwa Liu
Summary: A dual CA9-targeted probe for detecting hypoxic CRC cells was synthesized and evaluated, showing good CA9-targeting ability, high stability, and affinity both in vitro and in vivo.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Medicine, Research & Experimental
Shuo Yang, Wenbo Liao
Summary: The study revealed that hydroxysafflor yellow A (HSYA) plays a protective role in intervertebral disc degeneration by alleviating oxidative stress injury and apoptosis, as well as improving the regulation of extracellular matrix balance.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Multidisciplinary Sciences
Timothy Samuel, Sara Rapic, Cristiana O'Brien, Michael Edson, Yuan Zhong, Ralph S. DaCosta
Summary: Pancreatic cancer is a deadly disease with limited treatment options. This study developed a novel intravital fluorescence microscopy platform to study tumor cell hypoxia within the pancreatic tumor microenvironment (TME) in vivo. The study used a hypoxia-response element (HRE)/green fluorescent protein (GFP) reporter to demonstrate that HRE/GFP is a reliable biomarker of pancreatic tumor hypoxia, responding dynamically to changing oxygen concentrations. The study also analyzed the spatial relationships between tumor hypoxia, microvasculature, and tumor-associated collagen structures using in vivo second harmonic generation microscopy.
Article
Multidisciplinary Sciences
Laura Capasso, Manuel Aranda, Guoxin Cui, Melanie Pousse, Sylvie Tambutte, Didier Zoccola
Summary: This study identified calcification-related ion transporters and other candidate genes in ahermatypic non-symbiotic scleractinian corals using tissue micro-dissection and RNA-sequencing. The research found several known calcification-related candidate genes in these corals and also identified several ion transporter candidate genes that play a role in scleractinian calcification for the first time.
SCIENTIFIC REPORTS
(2022)
Article
Physics, Multidisciplinary
Fei Li, Yuqin Yao
Summary: Based on the high-order restricted flows of the extended fifth-order KdV equation, the efKdV equation with self-consistent sources (efKdVESCS) is presented and its Lax pair is constructed. Two types of Nth Darboux transformations for the efKdVESCS are constructed. Various types of solutions including one-soliton, two-soliton, and rational solution are explicitly obtained using Darboux transformations.
THEORETICAL AND MATHEMATICAL PHYSICS
(2022)
Article
Cell Biology
Xiaolin Ai, Zengpanpan Ye, Chaoxin Xiao, Jian Zhong, Joseph J. Lancman, Xuelan Chen, Xiangyu Pan, Yu Yang, Lin Zhou, Xiang Wang, Huashan Shi, Dongmei Zhang, Yuqin Yao, Dan Cao, Chengjian Zhao
Summary: Accurate prediction of intracranial infiltration tendency and drug response of individual GBM cells is crucial for personalized prognosis and treatment. The zebrafish PDOX model provides a novel avenue for precision medicine of GBM, allowing for prediction of long-term outcomes within a short period of time.
DISEASE MODELS & MECHANISMS
(2022)
Article
Immunology
Manyu Zhao, Liqun Wang, Mengzhu Wang, Qixue Bao, Rui Qian, Lijun Peng, Aiping Fang, Wen Du, Linshen Xie, Zunzhen Zhang, Yuqin Yao, Ben Zhang
Summary: This study demonstrated that PGRN levels were significantly increased in a silicosis mouse model. PGRN was mainly expressed in alveolar macrophages and promoted the transcription of pro-inflammatory cytokines, with IL-6 showing the most significant increase. Knockdown of PGRN blocked the elevation of IL-6 in silicosis-induced alveolar macrophages. Furthermore, PGRN was found to increase the phosphorylation of Creb1, which is a transcriptional regulator of IL-6. These findings suggest that PGRN may play a key role in silica-induced inflammation and provide new insights for the diagnosis and drug therapy of silicosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Physics, Applied
Yehui Huang, Jingjing Di, Yuqin Yao
Summary: This paper studies a 2 x 2 matrix partial derivative problem and obtains the Lax pair by constructing a spectral transformation matrix. The Hirota equation with self-consistent sources is derived by considering the nonanalytic part of the dispersion relation. Furthermore, explicit solutions including N-soliton solutions are computed by the partial derivative-dressing method, and N-soliton solutions of the Hirota equation with self-consistent sources are obtained using the properties of the Cauchy matrix. The dynamic behavior of soliton solutions is analyzed.
INTERNATIONAL JOURNAL OF MODERN PHYSICS B
(2022)
Article
Chemistry, Medicinal
Hao Cui, Zan Hu, Kang Yang, Jingkun Huang, Yichao Wu, Quanwei Chen, Ran Wei, Penfeng Wang, Hui Wang, Hongmei Li, Yadong Chen, Tao Lu, Yuqin Yao, Yong Zhu
Summary: Activation of the TRAIL proapoptotic pathway can promote cancer cell apoptosis. Histone deacetylases (HDACs) are promising drug targets for cancers. The development of highly TRAIL-sensitive HDAC inhibitors that can synergize with TRAIL may be a promising strategy for cancer treatment. Compound IIc showed good inhibitory activity against HDAC1 and HCT116 cells and displayed higher sensitivity than ONC201 in activating TRAIL expression and promoting apoptosis. In a xenograft model, compound IIc exhibited significantly greater inhibitory activity compared to positive control drugs. These findings suggest the potential of highly TRAIL-sensitive HDAC inhibitors as colorectal tumor cancer drugs.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Engineering, Mechanical
Yehui Huang, Jingjing Di, Yuqin Yao
Summary: The defocusing Hirota equation with nonzero boundary condition is studied using the partial derivative-dressing method. A partial derivative-problem with non-canonical normalization conditions is introduced. The Lax pair of the defocusing Hirota equation with nonzero boundary condition is derived using an asymptotic expansion method. N-soliton solutions are constructed under a selected special spectral transformation matrix, and the dynamic behavior of soliton solutions is analyzed.
NONLINEAR DYNAMICS
(2023)
Article
Chemistry, Medicinal
Xingping Su, Zui Tan, Guan Wang, Zhihao Liu, Cailing Gan, Lin Yue, Hongyao Liu, Yuting Xie, Yuqin Yao, Tinghong Ye
Summary: This study suggests that compounds 44 and 52 may serve as potential lead compounds for the treatment of idiopathic pulmonary fibrosis.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Rheumatology
Li Zhang, Wenqiang Zhang, Chenghan Xiao, Xueyao Wu, Huijie Cui, Peijing Yan, Chao Yang, Mingshuang Tang, Yutong Wang, Lin Chen, Yunjie Liu, Yanqiu Zou, Lars Alfredsson, Lars Klareskog, Yanfang Yang, Yuqin Yao, Jiayuan Li, Zhenmi Liu, Chunxia Yang, Xia Jiang, Ben Zhang
Summary: A comprehensive genome-wide cross-trait analysis revealed the genetic overlaps, pleiotropic loci, and causal relationships between obesity, serum urate, and gout.
Article
Toxicology
Shanshan Zhao, Qixue Bao, Guochen Ma, Yuqin Yao, Linshen Xie, Jingyuan Xiong
Summary: In this study, we investigated the effects of benzo[b]fluoranthene (B[b]F), a main component of polycyclic aromatic hydrocarbons (PAHs), on blood-brain barrier (BBB) in an in vitro model. The results showed that B[b]F inhibited cell proliferation, induced apoptosis, increased levels of reactive oxygen species (ROS), and disrupted mitochondrial membrane potential (MMP) in mouse brain microvascular endothelial cells. Moreover, B[b]F treatment reduced the expression of BBB marker proteins Occludin and Claudin-5, suggesting that it may damage the BBB.
TOXICOLOGY IN VITRO
(2023)
Article
Nutrition & Dietetics
Liqun Wang, Manyu Zhao, Rui Qian, Mengzhu Wang, Qixue Bao, Xuxi Chen, Wen Du, Ling Zhang, Tinghong Ye, Yongmei Xie, Ben Zhang, Lijun Peng, Yuqin Yao
Summary: In this study, it was found that NMN alleviates silica-induced oxidative stress and lung injury by reducing reactive oxygen species levels, increasing glutathione levels, inhibiting the recruitment of inflammatory cells and inflammatory infiltration, as well as regulating the expression and nuclear translocation of antioxidant genes through the interaction with nuclear factor-erythroid 2 related factor 2 (Nrf2).
Article
Biochemistry & Molecular Biology
Ying Lu, Mengzhu Wang, Manyu Zhao, Qianru Zhang, Rui Qian, Zan Hu, Qi Ke, Lin Yu, Liqun Wang, Qinhuai Lai, Zhenmi Liu, Xia Jiang, Ben Zhang, Jinliang Yang, Yuqin Yao
Summary: Non-alcoholic steatohepatitis (NASH) is a chronic liver disease characterized by steatosis, inflammation, and fibrosis. This study found that the expression of Filamin A (FLNA) was increased in liver tissues of NASH patients and mice with NAFLD/NASH. Knocking down FLNA reduced the inflammatory response in macrophages and decreased fibrotic cytokines in hepatic stellate cells, suggesting FLNA's role in the pathogenesis of NASH.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Computer Science, Information Systems
Yuqin Yao, Hua Meng, Yang Gao, Zhiguo Long, Tianrui Li
Summary: Dimensionality reduction is a significant data preprocessing technique. This article analyzes the drawbacks of the traditional linear unsupervised dimensionality reduction method LPP and proposes an improved model to resolve them. Furthermore, the article enhances LPP to maintain topological connectivity of data. Experimental results demonstrate that the new model outperforms the original LPP model and other classic linear or non-linear dimensionality reduction methods.
INFORMATION SCIENCES
(2023)
Article
Toxicology
Qixue Bao, Shanshan Zhao, Yongqi Liu, Yuqin Yao, Jia You, Jingyuan Xiong
Summary: This study investigated the cytotoxicity of BbF on human lung epithelial cells and bronchial epithelial cells, and found that BbF inhibited cell growth and induced apoptosis through the ROS-mediated mitochondria disruption pathway.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Engineering, Mechanical
Yuqin Yao, Hui Zhou, Fei Li
Summary: Based on higher-order restricted flows, the first type of integrable deformed fourth-order matrix NLS equations, called FMNLSSCS, is derived. By using the partial derivative-dressing method, the second type of integrable deformed fourth-order matrix NLS equations, called FMNLS-MB, is presented. The equivalence between FMNLSSCS and FMNLS-MB is successfully proved and N-soliton solutions are obtained explicitly using the Cauchy matrix method.
NONLINEAR DYNAMICS
(2023)
Article
Respiratory System
Cailing Gan, Qianyu Zhang, Hongyao Liu, Guan Wang, Liqun Wang, Yali Li, Zui Tan, Wenya Yin, Yuqin Yao, Yongmei Xie, Liang Ouyang, Luoting Yu, Tinghong Ye
Summary: Nifuroxazide (NIF) shows potential as a treatment option for idiopathic pulmonary fibrosis (IPF) by inhibiting and reversing the development of pulmonary fibrosis. The findings of this study are important for exploring pharmaceutical treatments for pulmonary fibrosis.
RESPIRATORY RESEARCH
(2022)
