Article
Biochemistry & Molecular Biology
Hannah L. L. Smith, Juliet C. C. Gray, Stephen A. A. Beers, Janos M. M. Kanczler
Summary: Osteosarcoma, a bone cancer typically found in adolescents, is closely related to the conversion of bone marrow during adolescence. This study compared the differentiation potential of bone marrow cells from different regions with osteosarcoma cell lines. The results showed distinct differences in the differentiation abilities of hematopoietic and adipocyte-rich bone marrow, which correlated with specific characteristics of the osteosarcoma cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Viktorija Avilkina, Damien Leterme, Guillaume Falgayrac, Jerome Delattre, Flore Miellot, Veronique Gauthier, Christophe Chauveau, Olfa Ghali Mhenni
Summary: This study demonstrates, for the first time, that the duration and severity of energy deficits are critical for changes in bone parameters, BMSC differentiation, and Sirt1 expression.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biology
Zengdi Zhang, Zan Huang, Mohamed Awad, Mohammed Elsalanty, James Cray, Lauren E. Ball, Jason C. Maynard, Alma L. Burlingame, Hu Zeng, Kim C. Mansky, Hai-Bin Ruan
Summary: In mammals, the interaction between bone marrow stroma and hematopoietic progenitors contributes to bone-BM homeostasis, but the mechanisms and interactions between the development of skeletal and hematopoietic systems are largely unknown. This study reveals that the intracellular O-GlcNAc modification plays a role in dictating the fate and function of early BM stromal cells (BMSCs). O-GlcNAcylation promotes osteogenic differentiation of BMSCs and supports lymphopoiesis, while inhibiting adipogenesis and myelopoiesis. Impaired bone formation and defective hematopoiesis were observed when O-GlcNAc transferase (OGT) was ablated in BMSCs.
Article
Biology
Zheting Liao, Yu Jin, Yuhao Chu, Hansen Wu, Xiaoyu Li, Zhonghao Deng, Shuhao Feng, Nachun Chen, Ziheng Luo, Xiaoyong Zheng, Liangxiao Bao, Yongqing Xu, Hongbo Tan, Liang Zhao
Summary: A study using single-cell RNA sequencing explored the transcriptomic landscape of alcohol-induced osteonecrosis of the femoral head (ONFH). The study revealed the relevance of stromal and endothelial cells to the pathogenesis of the disease and provided resources for developing cell type-specific therapeutic strategies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rui D. Alvites, Mariana V. Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Justina Prada, Isabel Pires, Giulia Ronchi, Stefania Raimondo, Ana L. Luis, Stefano Geuna, Artur Severo P. Varejao, Ana Colette Mauricio
Summary: Cell secretome is of great interest in Regenerative Medicine. This study analyzed the secretome collected from Olfactory Mucosa Mesenchymal Stem Cells and Olfactory Ensheathing Cells and applied it to promote peripheral nerve regeneration. The results showed that this therapeutic combination had beneficial effects on nerve and muscle regeneration, but further research is needed to address irregularities in the outcomes.
Article
Cell & Tissue Engineering
Angela W. Xie, Nicholas A. Zacharias, Bernard Y. K. Binder, William L. Murphy
Summary: This study compared MSC aggregates generated via three different methods and found differences in their structure and immunomodulatory phenotype under resting conditions and in response to inflammatory stimulus. The methods with faster aggregation kinetics formed aggregates with higher cell packing density and reduced ECM synthesis.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Polymer Science
Ci Li, Meng Zhang, Song-Yang Liu, Feng-Shi Zhang, Teng Wan, Zhen-Tao Ding, Pei-Xun Zhang
Summary: This study demonstrated the effectiveness of constructing peripheral nerve conduits based on chitosan acetylation, showing good biocompatibility and mechanical properties. Culturing bone marrow mesenchymal stem cells (BMSCs) as spheroids improves therapeutic potential, with BMSCs spheroids exhibiting better results in the PNI rat model with small gap tubulization. Chitin nerve conduits with BMSCs spheroids provide a promising therapy option for peripheral nerve regeneration.
Article
Biochemistry & Molecular Biology
Ye Xiao, Guang-Ping Cai, Xu Feng, Yu-Jue Li, Wan-Hui Guo, Qi Guo, Yan Huang, Tian Su, Chang-Jun Li, Xiang-Hang Luo, Yong-Jun Zheng, Mi Yang
Summary: Senescence and altered differentiation potential of bone marrow stromal cells (BMSCs) lead to age-related bone loss. The splicing factor Y-box binding protein 1 (YBX1) regulates BMSC senescence and differentiation shift during aging by controlling RNA splicing. Deletion of Ybx1 in BMSCs accelerates bone loss in mice, while its overexpression stimulates bone formation. Sciadopitysin, a small compound, attenuates the degradation of YBX1 and prevents bone loss in old mice.
Article
Engineering, Biomedical
Anamika Singh, Alok Raghav, Parvaiz Ahmad Shiekh, Ashok Kumar
Summary: This study developed a treatment approach for diabetic peripheral neuropathy (DPN) using a fused exosomal system combined with electrical stimulation, showing normalization of nerve conduction velocity and compound muscle action potential, muscle recovery, and observed paracrine effects in controlling hyperglycemia and tissue damage attenuation. It provides a promising effective treatment for DPN and contributes to cutting-edge therapeutic approaches.
BIOACTIVE MATERIALS
(2021)
Review
Endocrinology & Metabolism
Yuki Matsushita, Wanida Ono, Noriaki Ono
Summary: Bone marrow contains precursor cells for osteoblasts and adipocytes. Recent studies have identified subsets of bone marrow stromal cells (BMSCs) that can differentiate into marrow adipocytes and possess diverse functions in homeostasis and regeneration. The trajectory of BMSCs towards marrow adipocytes is influenced by multiple modes of cell-autonomous and non-cell autonomous regulations.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
C. Yue, J. Cao, A. Wong, J. H. Kim, S. Alam, G. Luong, S. Talegaonkar, Z. Schwartz, B. D. Boyan, W. Giannobile, S. E. Sahingur, Z. Lin
Summary: This study found that exosomes derived from human bone marrow stromal cells can suppress the progression of periodontitis and reduce inflammation and tissue destruction. This suggests that hBMSC exosomes have the potential to be used as a therapeutic approach in the management of periodontitis.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Immunology
Chiara Tomasoni, Corinne Arsuffi, Samantha Donsante, Alessandro Corsi, Mara Riminucci, Andrea Biondi, Alice Pievani, Marta Serafini
Summary: Acute myeloid leukemia (AML) cells have a negative impact on the osteogenic differentiation of normal bone marrow stromal cells (BMSCs), and direct interaction between AML cells and BMSCs plays a vital role in this process. AML-mediated activation of Notch signaling is involved in the ineffective osteogenic commitment of BMSCs, resulting in the formation of a pre-osteoblast-rich niche supportive of leukemia development in the bone marrow.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jian-Tong Cui, Xin-Yuan Wang, Xiao-Dan Mu, Meng Huang, Ya-Di Wang, Qiang Luo, Hui-Xia He
Summary: This study establishes a sustainably releasing system of exosomes-fibrin combinate loaded on tantalum-coating titanium implants. The system has potential effects on osseointegration between tantalum coating titanium implants and the surrounding bone tissue. The study demonstrates that the exosome sustained-release system with fibrin loading on the tantalum coating successfully promotes adhesion, proliferation, and osteogenic differentiation of BMSCs, and significantly enhances the osseointegrative capacity of the implants in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cheng-Chang Lu, Cheng-Jung Ho, Hsuan-Ti Huang, Sung-Yen Lin, Shih-Hsiang Chou, Pei-Hsi Chou, Mei-Ling Ho, Yin-Chun Tien
Summary: Both bone marrow mononuclear cells (BMMNCs) and bone marrow stromal cells (BMSCs) have comparable effects on cell repopulation and interfacial allograft-bone healing after allograft anterior cruciate ligament reconstruction (ACLR). Intra-articular delivery of BM cells has limited benefits on graft cell repopulation and may cause higher inflammation in the early stage, with fibrocartilage formation in the tendon-bone interface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Clinical Neurology
Onur Erdogan, Mustafa Sakar, Sueheyla Bozkurt, Fatih Bayrakli
Summary: We present a case of a 45-year-old female patient with a recurrent grade II oligodendroglioma in the wall of the fourth ventricle.
BRITISH JOURNAL OF NEUROSURGERY
(2023)
Article
Neurosciences
Ozkan Alan, Tugba Akin Telli, Tugba Basoglu, Rukiye Arikan, Nazim Can Demircan, Ozlem Ercelep, Mustafa Sakar, Suheyla Bozkurt, Beste Melek Atasoy, Faysal Dane, Mustafa Ibrahim Ziyal, Perran Fulden Yumuk
Summary: This study evaluated the prognostic value of the Prognostic Nutritional Index (PNI) in patients with recurrent glioblastoma who received systemic treatment. The results showed that patients with higher PNI had significantly longer overall survival, and PNI was found to be an independent prognostic factor.
Article
Cell Biology
Ilgin Cagnan, Mustafa Keles, Ayse Gokce Keskus, Melike Tombaz, Ozge Burcu Sahan, Fatima Aerts-Kaya, Duygu Uckan-Cetinkaya, Ozlen Konu, Aysen Gunel-Ozcan
Summary: Fanconi anemia (FA) is a rare genetic disorder characterized by genomic instability and bone marrow failure. This study investigated the miRNA profile of FA BM-MSCs before and after BM transplantation, with specific focus on miRNAs that may modulate BM mRNA networks in FA patients, revealing potential candidates for further research.
Article
Hematology
Michael H. Albert, Tiarlan Sirait, Dirk-Jan Eikema, Katerina Bakunina, Claudia Wehr, Felipe Suarez, Maria Laura Fox, Nizar Mahlaoui, Andrew R. Gennery, Arjan C. Lankester, Rita Beier, Maria Ester Bernardo, Venetia Bigley, Caroline A. Lindemans, Siobhan O. Burns, Ben Carpenter, Jaroslaw Dybko, Tayfun Gungor, Fabian Hauck, Su Han Lum, Dmitry Balashov, Roland Meisel, Despina Moshous, Ansgar Schulz, Carsten Speckmann, Mary A. Slatter, Brigitte Strahm, Duygu Uckan-Cetinkaya, Isabelle Meyts, Tanja C. Vallee, Robert Wynn, Benedicte Neven, Emma C. Morris
Summary: Allogeneic hematopoietic stem cell transplantation (HSCT) is the gold standard curative therapy for infants and children with inborn errors of immunity (IEI), but its adoption for adolescents and adults with IEI has been delayed due to lack of outcome data and perceived high risk of transplant-related mortality. This large retrospective, multicenter study reports on 329 IEI patients who underwent HSCT between 2000 and 2019. The study found that the overall survival (OS) and event-free survival (EFS) rates were 78% and 65% at 1 year post-HSCT, and 71% and 62% at 5 years post-HSCT, respectively. The study also identified risk factors for adverse outcomes, providing important evidence for patient selection.
Letter
Biophysics
Baris Kuskonmaz, Deniz Ayvaz, Fatma Visal Okur, Burca Aydin, Ilhan Tezcan, Duygu Uckan Cetinkaya
BONE MARROW TRANSPLANTATION
(2022)
Article
Biophysics
M. Akif Yesilipek, Vedat Uygun, Alphan Kupesiz, Gulsun Karasu, Gulyuz Ozturk, Mehmet Ertem, Ilgen Sasmaz, Hayriye Daloglu, Elif Guler, Volkan Hazar, Tunc Fisgin, Gulay Sezgin, Savas Kansoy, Baris Kuskonmaz, Burcu Akinci, Namik Ozbek, Elif Unal Ince, Seda Ozturkmen, Funda Tayfun Kupesiz, Koray Yalcin, Sema Anak, Ceyhun Bozkurt, Musa Karakukcu, Serhan Kupeli, Davut Albayrak, Haldun Oniz, Serap Aksoylar, Fatma Visal Okur, Canan Albayrak, Fatma Demir Yenigurbuz, Ikbal Ok Bozkaya, Talia Ileri, Orhan Gursel, Barbaros Sahin Karagun, Gulen Tuysuz Kintrup, Suna Celen, Murat Elli, Basak Adakli Aksoy, Ebru Yilmaz, Atila Tanyeli, Sule Turan Akyol, Zuhal Onder Sivi, Gulcihan Ozek, Duygu Uckan, Ibrahim Kartal, Didem Atay, Arzu Akyay, Ozlem Arman Bilir, Hasan Fatih Cakmakl, Emin Kurekci, Baris Malbora, Sinan Akbayram, Haci Ahmet Demir, Suar Caki Kilic, Adalet Meral Gunes, Emine Zengin, Salih Ozmen, Ali Bulent Antmen
Summary: The study analyzed national data on hematopoietic stem cell transplantation for thalassemia major patients in Turkey, finding that transplanting before the age of 7 with a matched donor can improve outcomes, and that different time points and donor types have varying impacts on transplant results.
BONE MARROW TRANSPLANTATION
(2022)
Article
Genetics & Heredity
Basak Torun, Ahmet Bilgin, Diclehan Orhan, Rahsan Gocmen, Sebnem Sara Kilic, Baris Kuskonmaz, Duygu Cetinkaya, Ilhan Tezcan, Deniz Cagdas
Summary: PNP deficiency caused by mutations in the PNP gene can result in immunodeficiency and various clinical symptoms. Hematopoietic stem cell transplantation is the only available treatment and can improve neurological symptoms and developmental delay.
EUROPEAN JOURNAL OF MEDICAL GENETICS
(2022)
Article
Pediatrics
Anar Gurbanov, Bora Gulhan, Baris Kuskonmaz, Fatma Visal Okur, Fatih Ozaltin, Ali Duzova, Duygu Uckan Cetinkaya, Rezan Topaloglu
Summary: This study aimed to determine the incidence of kidney complications in pediatric allogeneic hematopoietic stem cell transplantation patients. The results showed a higher incidence of acute kidney injury, with primary malignant disease and viral infection being associated with an increased risk. Additionally, 24-hour ambulatory blood pressure monitoring played an important role in monitoring hypertension in these patients.
PEDIATRIC NEPHROLOGY
(2023)
Article
Genetics & Heredity
Burak Kahveci, Selin Onen, Fuat Akal, Petek Korkusuz
Summary: In this study, a deep learning method was used to detect and count seminiferous tubules and spermatogonial stem/progenitor cells (SSPCs) in testis sections of newborn mice. This provides a rapid and easy detection method for clinicians dealing with male infertility caused by prepubertal testicular damage.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Clinical Neurology
Fatma Gokcem Yildiz, Cagri Mesut Temucin
Summary: This study aimed to investigate corticospinal excitability using multi-modal conditioning paradigms of combined somatosensory electrical and visual stimulation to understand sensory-motor integration. The results showed that visual inputs have significant modulatory effects on corticospinal excitability, and the integration of visual and somatosensory stimuli may occur via different neural circuits and/or interneuron populations.
NEUROPHYSIOLOGIE CLINIQUE-CLINICAL NEUROPHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Eda Ciftci, Naz Bozbeyoglu, Ihsan Gursel, Feza Korkusuz, Feray Bakan Misirlioglu, Petek Korkusuz
Summary: This study compares the efficiency of magnetically activated cell sorting and ultracentrifugation for isolating exosomes derived from human mesenchymal stem cells. The results show that magnetically activated cell sorting provides higher purity and quantity of exosomes, and offers a quick and reliable method for potential cellular therapeutic approaches.
Article
Cell & Tissue Engineering
Selin Onen, Ali Can Atik, Merve Gizer, Sevil Kose, Onder Yaman, Haluk Kulah, Petek Korkusuz
Summary: A new microfluidic device based on bone marrow-derived mesenchymal stem cells has been found to support the maintenance of stem cells and in vitro spermatogenesis, offering potential protection and expansion of the stem cell pool for future fertility preservation in pediatric cancer survivors.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Orthopedics
Eda Ciftci Dede, Merve Gizer, Feza Korkusuz, Zeynep Bal, Hiroyuki Ishiguro, Hideki Yoshikawa, Takashi Kaito, Petek Korkusuz
Summary: In this study, we combined nano-hydroxyapatite-polyethylene glycol/poly(lactic acid) (nHAp-PEG/PLA) with recombinant human bone morphogenetic protein 2 (rhBMP2) and evaluated the effects of 0.5, 1.0, and 3.0 μg/mL rhBMP2 on the proliferation, differentiation, and osteogenic genes of human bone marrow mesenchymal stem cells (hBMMSCs). The results showed that nHAp-PEG/PLA composites with 1.0 μg/mL and 3.0 μg/mL rhBMP2 promoted hBMMSC proliferation and differentiation.
Article
Chemistry, Analytical
Ozge Boyacioglu, Tuba Recber, Sedef Kir, Petek Korkusuz, Emirhan Nemutlu
Summary: Synthetic and natural cannabinoid derivatives have potential as drug candidates for various diseases, including cancer. ACPA, a synthetic cannabinoid, shows anticancer effects on lung, pancreatic, and endometrial cancer. Therefore, it is crucial to quantify ACPA in biological samples for monitoring its effects. However, the short half-life and lack of UV signal of ACPA present challenges in its quantification. In this study, a sensitive and selective LC-MS/MS method was developed for quantification of ACPA in cell culture medium and intracellular matrix.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2023)
Article
Clinical Neurology
Mustafa Sakar, Onur Erdogan, Suheyla Uyar Bozkurt, Adnan Dagcinar
Summary: This study presents the first case of intermediate-grade meningeal melanocytoma (IGM) in a 19-month-old girl. The surgery was complicated and required a second surgery for reconstruction and dural repair. Close monitoring with radiological imaging is crucial for such cases.
TURKISH NEUROSURGERY
(2022)