Article
Engineering, Biomedical
Sang-Yoon Lee, Bongju Kim, Sung-Ho Lee, Kyungwon Ju, Soung-Min Kim, Jong-Ho Lee, KangMi Pang
Summary: This study evaluated the stimulating effect of pulsed electromagnetic field (PEMF) on the regeneration of crush-injured rat sciatic nerve when combined with bone marrow mesenchymal stem cells (BMSCs) and recombinant human nerve growth factor (rhNGF-beta). The results showed that the group treated with PEMF + NGF-Ad + BMSC had the highest number of axons. Therefore, combining PEMF with BMSC and NGF-Ad can be considered as a treatment method for nerve regeneration.
BIOMEDICAL ENGINEERING LETTERS
(2023)
Article
Cell & Tissue Engineering
Jie Chen, Huaxing Xu, Kun Xia, Shuhua Cheng, Qi Zhang
Summary: In this study, it was found that RvE1 promotes pulp inflammation resolution and dentin regeneration, and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. The effects of RvE1 on DPSCs were mediated at least partially through AKT, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 shows promising application potential in regenerative endodontics.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jonas Flatscher, Elizabeth Pavez Lorie, Rainer Mittermayr, Paul Meznik, Paul Slezak, Heinz Redl, Cyrill Slezak
Summary: Environmental biophysical interactions have a significant impact on trauma recovery processes in the human body. Studies on Pulsed Electromagnetic Fields (PEMF) have provided insights into their effects on the human body, cells, and biological systems. However, the diverse study setups have made it challenging to interpret commonalities and understand the underlying mechanisms of this treatment. This review provides a comprehensive overview of the published literature on PEMF, offering a scaffolded summary for future research and clinical use, and highlighting the need for common reporting parameters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Julia K. Bar, Anna Lis-Nawara, Tomasz Kowalczyk, Piotr G. Grelewski, Sandra Stamnitz, Hanna Gerber, Aleksandra Klimczak
Summary: This study investigates the osteogenic potential of human dental pulp stem cells (hDPSCs) grown on different scaffolds. Results show that a hydrolytically modified poly (L-lactide-co-caprolactone) (PLCL) electrospun scaffold provides a better environment for hDPSCs attachment and osteogenic differentiation compared to a non-woven hyaluronic acid (HYAFF-11 (TM)) mesh scaffold. The findings suggest that hDPSC/PLCL constructs could be a potential innovative approach for bone defect repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Orthopedics
Ming Wang, Yucong Li, Lu Feng, Xiaoting Zhang, Haixing Wang, Nianli Zhang, Ingmar Viohl, Gang Li
Summary: PEMF treatment promotes meniscal healing and prevents osteoarthritis progression in a rat model.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Matej Kranjc, Tamara Polajzer, Vitalij Novickij, Damijan Miklavcic
Summary: High-Intensity Pulsed Electromagnetic Fields (HI-PEMF) treatment, a noninvasive technique, has shown potential for transferring genetic materials and activating immune response through remote induction of electric field. This study investigates the release of DAMP molecules after HI-PEMF treatment and suggests minimal activation of immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Anatomy & Morphology
Marzieh Darvishi, Hatef Ghasemi Hamidabadi, Maryam Nazm Bojnordi, Sara Saeednia, Maria Zahiri, Ali Niapour, Rafieh Alizadeh
Summary: Stem cell therapy is a potential treatment for spinal cord injury, with research focusing on the differentiation of Human Dental Pulp Stem Cells (hDPSCs) into functional motor neuron like cells (MNLCs) and promoting neuroregeneration in adult spinal cord slice cultures. The study found that hDPSCs can be differentiated into motor neuron phenotype and successfully integrate into spinal slices, showing potential for future therapeutic applications.
Review
Biochemistry & Molecular Biology
Lorenzo Moretti, Davide Bizzoca, Alessandro Geronimo, Andrea Michele Abbaticchio, Francesco Luca Moretti, Arianna Carlet, Francesco Fischetti, Biagio Moretti
Summary: Chondropathy of the knee is a common degenerative cartilage condition that affects aging individuals. Recent scientific research has identified new therapies that target adenosine A2 receptors, which have protective effects against cell damage. Intra-articular injections of polydeoxyribonucleotides (PDRN) and Pulsed Electromagnetic Fields (PEMF) have shown regenerative and healing effects by stimulating the adenosine signal. This review highlights the beneficial effects of PDRN injections in reducing pain and improving functional scores, as well as the potential of PEMF therapy in treating various knee pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Mattia Di Bartolomeo, Francesco Cavani, Arrigo Pellacani, Alexis Grande, Roberta Salvatori, Luigi Chiarini, Riccardo Nocini, Alexandre Anesi
Summary: Pulsed electromagnetic fields (PEMFs) are a type of biophysical stimulation that have shown promising results in improving bone regeneration and preventing bone loss. However, there is limited understanding of the underlying biological mechanisms and the effects on bone healing. The variability in the characteristics of electromagnetic fields and the responsiveness of cells involved in bone healing pose challenges in drawing definitive conclusions. This review summarizes the current research on PEMF stimulation in animal models with bone impairment, highlighting the need for standardized experimental guidelines and further controlled trials.
Article
Cell Biology
Masaki Hata, Maiko Omi, Yasuko Kobayashi, Nobuhisa Nakamura, Megumi Miyabe, Mizuho Ito, Tasuku Ohno, Yuka Imanishi, Tatsuhito Himeno, Hideki Kamiya, Jiro Nakamura, Hitoshi Miyachi, Shogo Ozawa, Ken Miyazawa, Akio Mitani, Toru Nagao, Shigemi Goto, Jun Takebe, Tatsuaki Matsubara, Keiko Naruse
Summary: The study evaluated the long-term effects of a single transplantation of hDPSCs on diabetic polyneuropathy. The analysis at 16 weeks post-transplantation showed that DPSC transplantation significantly improved nerve conduction velocity, decreased blood flow, and increased sensory perception thresholds. Additionally, the DPSC-conditioned medium promoted neurite outgrowth of neurons.
Article
Dentistry, Oral Surgery & Medicine
L. Yao, F. Li, C. Yu, H. Wang, Y. Wang, L. Ye, F. Yu
Summary: The influence of chronological and replicative senescence on CD51(+)/PDGFR-alpha(+) hDPSCs was investigated using various experimental methods. Chronological senescence led to decreased CD51(+)/PDGFR-alpha(+) hDPSCs, impaired self-renewal, and higher ossificatory differentiation. Replicative senescence resulted in decreased CD51 expression, upregulated PDGFR-alpha expression, and weakened self-renewal and osteogenic differentiation. These findings highlight the distinct outcomes and core dysfunction of chronological and replicative senescence in CD51(+)/PDGFR-alpha(+) hDPSCs.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
Songhee Cheon, Jungyoon Kim, Misun Kang
Summary: This study investigated the effect of pulsed electromagnetic field on proprioception in people with functional flat feet. The results showed that short-term application of PEMF can improve the balance ability of individuals with functional flat feet.
JOURNAL OF MAGNETICS
(2023)
Article
Medicine, Research & Experimental
Violet Man-Chi Ko, Xin He, Sai-Chuen Fu, Patrick Shu-Hang Yung, Samuel Ka-Kin Ling
Summary: This study aims to investigate the treatment effects of PEMF for Achilles tendinopathy. By increasing patient compliance with the rehabilitation program, PEMF can alleviate pain, improve function, and restore mechanical properties of the tendon.
Article
Cell & Tissue Engineering
Xiaoping Hu, Li Wang, Yuanqiao He, Minli Wei, Huilin Yan, Hongshui Zhu
Summary: Chlorogenic acid (CGA) can promote the osteogenic differentiation of human dental pulp stem cells (hDPSCs) by regulating Wnt signaling. These findings will pave the way for further research on repairing defective alveolar bone in patients with periodontal disease.
STEM CELLS AND DEVELOPMENT
(2021)