Article
Biotechnology & Applied Microbiology
Aishwarya Saraswat, Ketan Patel
Summary: This study aimed to find the optimal lipid-based nanovector for gene delivery by testing various types. The study found that lipid nanoparticles (LNPs) were more effective at delivering plasmid DNA than nanocomplexes and also provided better protection. The best performing LNP contained the biodegradable lipid sphingomyelin 102. Therefore, the optimized LNP formulation using sphingomyelin 102 could be a highly efficient nonviral gene-delivery system for targeting drug-resistant melanoma.
Article
Pharmacology & Pharmacy
Zeynep Burcu Akkus-Dagdeviren, Sema Arisoy, Julian David Friedl, Andrea Fuerst, Ahmad Saleh, Andreas Bernkop-Schnuerch
Summary: The aim of this study was to develop enzyme-activated charge-reversal lipid nanoparticles (LNPs) as novel gene delivery systems. The researchers successfully anchored a transfection promoter on the surfaces of LNPs to achieve high encapsulation and efficient gene delivery. The experiment results showed that the enzyme-triggered charge reversal of LNPs resulted in significantly increased transfection efficiency in human alveolar epithelial cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Jaidev Chakka, Mohammed Maniruzzaman
Summary: In this study, a novel extrusion-based Sprayed Multi Adsorbed-droplet Reposing Technology (SMART) was used to prepare 150 nm plasmid DNA nanoparticles, which were successfully applied in vitro transfection experiments with cancer cells.
MOLECULAR PHARMACEUTICS
(2023)
Review
Pharmacology & Pharmacy
Shintaro Fumoto, Tsuyoshi Yamamoto, Kazuya Okami, Yuina Maemura, Chisato Terada, Asako Yamayoshi, Koyo Nishida
Summary: Nucleic acid and genetic medicines show great potential in treating various intractable diseases. Understanding their in vivo fate is crucial for rational design and development of drugs. Plasma protein binding can be utilized to manipulate pharmacokinetics, while ligand conjugation method is widely used for controlling drug distribution in the body.
Article
Acoustics
Maryam Zolghadrnasab, Amir Mousavi, Abbas Farmany, Ayyoob Arpanaei
Summary: This study synthesized PEI-coated MSNs for efficient delivery of GUS-encoding plasmid DNA into tobacco cells using ultrasound treatment. It was found that high levels of sonoporation may affect cell viability adversely. Optimal conditions for ultrasonic treatment were determined as 8 minutes at 160, 320, and 640 W, showing the potential for an economical and straightforward approach for gene transfer in plant cells.
ULTRASONICS SONOCHEMISTRY
(2021)
Editorial Material
Multidisciplinary Sciences
Roberto Di Blasi, Masue M. Marbiah, Velia Siciliano, Karen Polizzi, Francesca Ceroni
Summary: Transient transfections are commonly used in basic and synthetic biology studies to investigate pathway regulation and circuit design, but resource competition for gene expression can lead to biases in data interpretation, emphasizing the need for better transfection controls to ensure reliable results.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Mckenna G. G. Hanson, Christian J. J. Grimme, Nicholas W. W. Kreofsky, Sidharth Panda, Theresa M. M. Reineke
Summary: Nucleic acid-based medicines and vaccines are playing an increasingly important role in our therapeutic toolbox. Antisense oligonucleotides (ASOs) are a key genetic medicine that can downregulate protein production. However, they need a delivery vehicle to enter cells. This study aimed to develop a method to rapidly discover new micelle formulations using mixed diblock polymers, which show improved delivery efficiency compared to linear nonmicelle variants.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Erica Quagliarini, Junbiao Wang, Serena Renzi, Lishan Cui, Luca Digiacomo, Gianmarco Ferri, Luca Pesce, Valentina De Lorenzi, Giulia Matteoli, Heinz Amenitsch, Laura Masuelli, Roberto Bei, Daniela Pozzi, Augusto Amici, Francesco Cardarelli, Cristina Marchini, Giulio Caracciolo
Summary: Lipid nanoparticles (LNPs) have shown promise as effective gene delivery vehicles, with a specific formulation (LNP15) demonstrating successful transfection and potential application in cancer vaccines.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Lyes Toualbi, Maria Toms, Mariya Moosajee
Summary: Inherited retinal diseases (IRDs) are a group of disorders causing progressive vision loss and affecting approximately one in 1000 people worldwide. While gene augmentation therapy using viral vectors has shown promise, non-viral gene augmentation strategies using plasmids with minimal bacterial backbones and S/MAR sequences offer potential advantages such as accommodating larger genes and reducing immune responses. Despite challenges like low retinal transfection rates, various approaches including using nanoparticles and electrical methods show promise in preclinical studies, suggesting non-viral gene therapy as a safer and effective option for future IRD treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Jamie L. Betker, Thomas J. Anchordoquy
Summary: It has been discovered that repeat administration of nanoparticles through intravenous administration can trigger an immune response that may threaten the efficiency of drug delivery and also pose a risk to life. This study examined the cytokine response at both the systemic and tissue levels following repeated administration of lipoplexes coated with either lactose or PEG. The findings show that the cytokine levels in the blood differ significantly from those observed in individual tissues, and while a reduced cytokine response was consistently observed with lactosylated particles, it did not result in improved delivery or expression compared to PEGylated formulations.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Diviya Santhanes, Alex Wilkins, Huiming Zhang, Robert John Aitken, Mingtao Liang
Summary: Lipid/polymer hybrid nanoparticles loaded with red fluorescent protein encoded plasmid DNA were successfully formulated using microfluidics. These nanoparticles showed high encapsulation efficiency and transfection efficacy, demonstrating their potential for clinical applications in DNA therapeutics.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Biochemical Research Methods
Andrew Hamann, Angela K. Pannier
Summary: Although human mesenchymal stem cells (hMSCs) have not yet been approved by the FDA for clinical use, research is ongoing to develop gene delivery methods for therapeutic applications. Strategies, such as innovative carriers and chemical and physical priming, have been developed to improve transfection efficiency in hMSCs. These strategies aim to optimize cell phenotypes for enhanced immunomodulation and tissue regeneration, as well as for functions like tumor-killing and tissue engineering.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Eivina Radzeviciute-Valciuke, Jovita Gecaite, Augustinas Zelvys, Aukse Zinkeviciene, Rokas Zalneravicius, Veronika Malysko-Ptasinske, Ausra Nemeikaite-Ceniene, Vytautas Kaseta, Natalija German, Jurij Novickij, Almira Ramanaviciene, Julita Kulbacka, Vitalij Novickij
Summary: This study demonstrates that MHz frequency bursts of nanosecond pulses can enhance gene delivery and investigates the potential of gold nanoparticles in this process. The combination of microsecond pulses and gold nanoparticles significantly improves gene delivery, depending on the surface charge and size of the nanoparticles. Gold nanoparticles are ineffective in nanosecond pulse treatment, but MHz pulse treatment remains competitive, with high gene delivery efficiency, low reactive oxygen species generation, preserved cellular viability, and easier operation.
Review
Materials Science, Multidisciplinary
Pei Huang, Hongzhang Deng, Yongfeng Zhou, Xiaoyuan Chen
Summary: This review summarizes the development of polymeric nanocarriers for mRNA delivery and discusses approaches to enhance mRNA transfection efficiency. The authors propose conclusions and perspectives on the clinical translation of mRNA therapy, believing that the introduction of mRNA will lead to promising techniques for disease prevention and treatment in the future.
Article
Pharmacology & Pharmacy
Hardy Mitdank, Simko Sama, Meike Troeger, Maria Francesca Testa, Mattia Ferrarese, Dario Balestra, Mirko Pinotti, Alexander Weng
Summary: Minicircle DNA is a promising tool in gene therapy with advantages such as higher transfection efficiency and longer expression time, but its cost-intensive production limits broader use. An optimized production method can lead to high-quality minicircle DNA, which has been investigated for pharmaceutical potential in vitro in a tumor cell model.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)