Journal
MITOCHONDRIAL DNA PART A
Volume 27, Issue 4, Pages 2826-2832Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/19401736.2015.1053114
Keywords
Gene rearrangement; long-range PCR; mitogenome; phylogeny
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Funding
- U.S. National Science Foundation (NSF) [EF-0531763]
- Lerner Gray Fund for Marine Research (AMNH)
- Constantine S. Niarchos Expedition Award
- Gerstner Family Foundation
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Six complete and three partial actiniarian mitochondrial genomes were amplified in two semicircles using long-range PCR and pyrosequenced in a single run on a 454 GS Junior, doubling the number of complete mitogenomes available within the order. Typical metazoan mtDNA features included circularity, 13 protein-coding genes, 2 ribosomal RNA genes, and length ranging from 17,498 to 19,727 bp. Several typical anthozoan mitochondrial genome features were also observed including the presence of only two transfer RNA genes, elevated A + T richness ranging from 54.9 to 62.4%, large intergenic regions, and group 1 introns interrupting NADH dehydrogenase subunit 5 and cytochrome c oxidase subunit I, the latter of which possesses a homing endonuclease gene. Within the sea anemone Alicia sansibarensis, we report the first mitochondrial gene order rearrangement within the Actiniaria, as well as putative novel non-canonical protein-coding genes. Phylogenetic analyses of all 13 protein-coding and 2 ribosomal genes largely corroborated current hypotheses of sea anemone interrelatedness, with a few lower-level differences.
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