What Macromolecular Crowding Can Do to a Protein

Tuning the architecture of three-dimensional collagen hydrogels by physiological macromolecular crowding

(2014) Jean-Yves Dewavrin et al.   Acta Biomaterialia

Intracellular crowding effects on the self-association of the bacterial cell division protein FtsZ

(2014) Lamis Naddaf et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Folding of an Unfolded Protein by Macromolecular Crowding in Vitro

(2014) Jörgen Ådén et al.   BIOCHEMISTRY

Crowding Activates ClpB and Enhances Its Association with DnaK for Efficient Protein Aggregate Reactivation

(2014) Ianire Martín et al.   BIOPHYSICAL JOURNAL

Minimal Effects of Macromolecular Crowding on an Intrinsically Disordered Protein: A Small-Angle Neutron Scattering Study

(2014) David P. Goldenberg et al.   BIOPHYSICAL JOURNAL

Macromolecular Crowding Effects on Two Homologs of Ribosomal Protein S16: Protein-Dependent Structural Changes and Local Interactions

(2014) Therese Mikaelsson et al.   BIOPHYSICAL JOURNAL

Macromolecular crowding decelerates aggregation of a β-rich protein, bovine carbonic anhydrase: a case study

(2014) Shruti Mittal et al.   JOURNAL OF BIOCHEMISTRY

Interactions of Macromolecular Crowding Agents and Cosolutes with Small-Molecule Substrates: Effect on Horseradish Peroxidase Activity with Two Different Substrates

(2014) William M. Aumiller et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Macromolecular Crowding Effect upon in Vitro Enzyme Kinetics: Mixed Activation–Diffusion Control of the Oxidation of NADH by Pyruvate Catalyzed by Lactate Dehydrogenase

(2014) Cristina Balcells et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Crowding effects on the temperature and pressure dependent structure, stability and folding kinetics of Staphylococcal Nuclease

(2014) M. Erlkamp et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

CaMKII Binding to GluN2B Is Differentially Affected by Macromolecular Crowding Reagents

(2014) Dayton J. Goodell et al.   PLoS One

Single-molecule spectroscopy reveals polymer effects of disordered proteins in crowded environments

(2014) Andrea Soranno et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assessment of the Effect of Macromolecular Crowding on Aggregation Behaviour of a Model Amyloidogenic Peptide

(2014) Bharti Gaharwar et al.   PROTEIN AND PEPTIDE LETTERS

Filament formation by metabolic enzymes is a specific adaptation to an advanced state of cellular starvation

(2014) Ivana Petrovska et al.   eLife

The crowd you're in with: Effects of different types of crowding agents on protein aggregation

(2013) Leonid Breydo et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Quantification of Excluded Volume Effects on the Folding Landscape of Pseudomonas aeruginosa Apoazurin In Vitro

(2013) Alexander Christiansen et al.   BIOPHYSICAL JOURNAL

Direct Observation of Protein Unfolded State Compaction in the Presence of Macromolecular Crowding

(2013) Therese Mikaelsson et al.   BIOPHYSICAL JOURNAL

Dual Specificity Kinase DYRK3 Couples Stress Granule Condensation/Dissolution to mTORC1 Signaling

(2013) Frank Wippich et al.   CELL

Behavior of Plasmodium falciparum purine nucleoside phosphorylase in macromolecular crowded environment

(2013) Manish Kumar Suthar et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Phase transitions and size scaling of membrane-less organelles

(2013) Clifford P. Brangwynne   JOURNAL OF CELL BIOLOGY

Molecular Crowding Stabilizes Both the Intrinsically Disordered Calcium-Free State and the Folded Calcium-Bound State of a Repeat in Toxin (RTX) Protein

(2013) Ana-Cristina Sotomayor-Pérez et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Molecular Crowding Enhanced ATPase Activity of the RNA Helicase eIF4A Correlates with Compaction of Its Quaternary Structure and Association with eIF4G

(2013) Sabine R. Akabayov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A nuclear F-actin scaffold stabilizes ribonucleoprotein droplets against gravity in large cells

(2013) Marina Feric et al.   NATURE CELL BIOLOGY

Macromolecular Crowding as a Suppressor of Human IAPP Fibril Formation and Cytotoxicity

(2013) Janine Seeliger et al.   PLoS One

Denatured State Structural Property Determines Protein Stabilization by Macromolecular Crowding: A Thermodynamic and Structural Approach

(2013) Shruti Mittal et al.   PLoS One

A Quantitative Model of ERK MAP Kinase Phosphorylation in Crowded Media

(2013) Kazuhiro Aoki et al.   Scientific Reports

Myelin Membrane Assembly Is Driven by a Phase Transition of Myelin Basic Proteins Into a Cohesive Protein Meshwork

(2013) Shweta Aggarwal et al.   PLOS BIOLOGY

Aqueous Phase Separation as a Possible Route to Compartmentalization of Biological Molecules

(2012) Christine D. Keating   ACCOUNTS OF CHEMICAL RESEARCH

Effects of macromolecular crowding on the structural stability of human α-lactalbumin

(2012) De-Lin Zhang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Inactivation of Recombinant Human Brain-Type Creatine Kinase During Denaturation by Guanidine Hydrochloride in a Macromolecular Crowding System

(2012) Yong-Qiang Fan et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Effects of Macromolecular Crowding on Burst Phase Kinetics of Cytochrome c Folding

(2012) Eefei Chen et al.   BIOCHEMISTRY

Effect of Molecular Crowding on the Temperature-Pressure Stability Diagram of Ribonuclease A

(2012) Yong Zhai et al.   CHEMPHYSCHEM

Effects of macromolecular crowding on refolding of recombinant human brain-type creatine kinase

(2012) Yong-Qiang Fan et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Macromolecular crowding modulates the kinetics and morphology of amyloid self-assembly by β-lactoglobulin

(2012) Baoliang Ma et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Myoglobin Unfolding in Crowding and Confinement

(2012) Ashima Malik et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Enzyme Activity in the Crowded Milieu

(2012) Tobias Vöpel et al.   PLoS One

The Contrasting Effect of Macromolecular Crowding on Amyloid Fibril Formation

(2012) Qian Ma et al.   PLoS One

Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior

(2012) Adam S. Zeiger et al.   PLoS One

Structure of Metaphase Chromosomes: A Role for Effects of Macromolecular Crowding

(2012) Ronald Hancock   PLoS One

Effects of Molecular Crowding on the Dynamics of Intrinsically Disordered Proteins

(2012) Elio A. Cino et al.   PLoS One

Effect of crowding by Ficolls on OmpA and OmpT refolding and membrane insertion

(2012) Cui Ye et al.   PROTEIN SCIENCE

Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies

(2011) Clarice Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

What is the true enzyme kinetics in the biological system? An investigation of macromolecular crowding effect upon enzyme kinetics of glucose-6-phosphate dehydrogenase

(2011) Matthew G.S. Norris et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Macromolecular Crowding Extended to a Heptameric System: The Co-chaperonin Protein 10

(2011) Ximena Aguilar et al.   BIOCHEMISTRY

Effects of Macromolecular Crowding on an Intrinsically Disordered Protein Characterized by Small-Angle Neutron Scattering with Contrast Matching

(2011) Daniel Johansen et al.   BIOPHYSICAL JOURNAL

Effects of Macromolecular Crowding on the Inhibition of Virus Assembly and Virus-Cell Receptor Recognition

(2011) Verónica Rincón et al.   BIOPHYSICAL JOURNAL

Limiting Amounts of Centrosome Material Set Centrosome Size in C. elegans Embryos

(2011) Markus Decker et al.   CURRENT BIOLOGY

Calcium-induced Folding of Intrinsically Disordered Repeat-in-Toxin (RTX) Motifs via Changes of Protein Charges and Oligomerization States

(2011) Ana Cristina Sotomayor-Pérez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

P granules extend the nuclear pore complex environment in theC. elegansgerm line

(2011) Dustin L. Updike et al.   JOURNAL OF CELL BIOLOGY

Macromolecular Crowding Fails To Fold a Globular Protein in Cells

(2011) Alexander P. Schlesinger et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Processive phosphorylation of ERK MAP kinase in mammalian cells

(2011) K. Aoki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Effect of Macromolecular Crowding, Ionic Strength and Calcium Binding on Calmodulin Dynamics

(2011) Qian Wang et al.   PLoS Computational Biology

Factors Defining Effects of Macromolecular Crowding on Protein Stability: An in Vitro/in Silico Case Study Using Cytochromec

(2010) Alexander Christiansen et al.   BIOCHEMISTRY

Understanding protein non-folding

(2010) Vladimir N. Uversky et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Increase of SARS-CoV 3CL peptidase activity due to macromolecular crowding effects in the milieu composition

(2010) Debora N. Okamoto et al.   BIOLOGICAL CHEMISTRY

Structural change of the enterobactin synthetase in crowded solution and its relation to crowding-enhanced product specificity in nonribosomal enterobactin biosynthesis

(2010) Zu-Feng Guo et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Crowding Effects on Association Reactions at Membranes

(2010) Jun Soo Kim et al.   BIOPHYSICAL JOURNAL

Hydrodynamic radius of polyethylene glycol in solution obtained by dynamic light scattering

(2010) K. L. Linegar et al.   COLLOID JOURNAL

Macromolecular crowding converts the human recombinant PrPC to the soluble neurotoxic β-oligomers

(2010) Liqin Huang et al.   FASEB JOURNAL

Macromolecular crowding effects on protein–protein binding affinity and specificity

(2010) Young C. Kim et al.   JOURNAL OF CHEMICAL PHYSICS

Effect of Crowding by Dextrans on the Hydrolysis ofN-Succinyl-l-phenyl-Ala-p-nitroanilide Catalyzed by α-Chymotrypsin

(2010) Isabel Pastor et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Macromolecular Crowding Remodels the Energy Landscape of a Protein by Favoring a More Compact Unfolded State

(2010) Jiang Hong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Macromolecular Crowding Tunes Folding Landscape of Parallel α/β Protein, Apoflavodoxin

(2010) Loren Stagg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Coilin-dependent snRNP assembly is essential for zebrafish embryogenesis

(2010) Magdalena Strzelecka et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Macromolecular Crowding Effects on Reactions of TePixD (Tll0078)†

(2010) Tsuguyoshi Toyooka et al.   PHOTOCHEMISTRY AND PHOTOBIOLOGY

Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding

(2010) Apratim Dhar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Non-linear effects of macromolecular crowding on enzymatic activity of multi-copper oxidase

(2009) Irina Pozdnyakova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Close encounters of the third kind: disordered domains and the interactions of proteins

(2009) Peter Tompa et al.   BIOESSAYS

Macromolecular Crowding Modulates Folding Mechanism of α / β Protein Apoflavodoxin

(2009) Dirar Homouz et al.   BIOPHYSICAL JOURNAL

Common Crowding Agents Have Only a Small Effect on Protein-Protein Interactions

(2009) Yael Phillip et al.   BIOPHYSICAL JOURNAL

Accelerated Fibrillation of α-Synuclein Induced by the Combined Action of Macromolecular Crowding and Factors Inducing Partial Folding

(2009) L. Munishkina et al.   Current Alzheimer Research

Linking folding and binding

(2009) Peter E Wright et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Crowded Cell-like Environment Accelerates the Nucleation Step of Amyloidogenic Protein Misfolding

(2009) Zheng Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Mysterious Unfoldome: Structureless, Underappreciated, Yet Vital Part of Any Given Proteome

(2009) Vladimir N. Uversky   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Modulation of Calmodulin Plasticity by the Effect of Macromolecular Crowding

(2009) Dirar Homouz et al.   JOURNAL OF MOLECULAR BIOLOGY

Effect of Macromolecular Crowding on Protein Folding Dynamics at the Secondary Structure Level

(2009) Smita Mukherjee et al.   JOURNAL OF MOLECULAR BIOLOGY

Residue-Specific Analysis of Frustration in the Folding Landscape of Repeat β/α Protein Apoflavodoxin

(2009) Loren Stagg et al.   JOURNAL OF MOLECULAR BIOLOGY

Nonadditive effects of mixed crowding on protein stability

(2009) Jyotica Batra et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Intrinsically Disordered Proteins in Human Diseases: Introducing the D2 Concept

(2008) Vladimir N. Uversky et al.   Annual Review of Biophysics

Guiding Protein Aggregation with Macromolecular Crowding†

(2008) Larissa A. Munishkina et al.   BIOCHEMISTRY

Macromolecular crowding and its potential impact on nuclear function

(2008) Karsten Richter et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Mixed macromolecular crowding inhibits amyloid formation of hen egg white lysozyme

(2008) Bing-Rui Zhou et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Binding of calcium ions to Ras promotes Ras guanine nucleotide exchange under emulated physiological conditions

(2008) Jun-Ming Liao et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Molecular Dynamics Studies of Polyethylene Oxide and Polyethylene Glycol: Hydrodynamic Radius and Shape Anisotropy

(2008) Hwankyu Lee et al.   BIOPHYSICAL JOURNAL

The unfoldomics decade: an update on intrinsically disordered proteins

(2008) A Keith Dunker et al.   BMC GENOMICS

Flexible nets: disorder and induced fit in the associations of p53 and 14-3-3 with their partners

(2008) Christopher J Oldfield et al.   BMC GENOMICS

Function and structure of inherently disordered proteins

(2008) A Keith Dunker et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Self-association of polynucleosome chains by macromolecular crowding

(2008) Ronald Hancock   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

A careful disorderliness in the proteome: Sites for interaction and targets for future therapies

(2008) Robert B. Russell et al.   FEBS LETTERS

Macromolecular Crowding Compacts Unfolded Apoflavodoxin and Causes Severe Aggregation of the Off-pathway Intermediate during Apoflavodoxin Folding

(2008) Ruchira Engel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Analytical Ultracentrifugation Studies of Phage ϕ29 Protein p6 Binding to DNA

(2008) Martín Alcorlo et al.   JOURNAL OF MOLECULAR BIOLOGY

Effects of Macromolecular Crowding on Glycoprotein Processing Enzymes

(2008) Kiichiro Totani et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Residue-Level Interrogation of Macromolecular Crowding Effects on Protein Stability

(2008) Lisa M. Charlton et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline: Germinal granules in mammals☆

(2008) Shinichiro Chuma et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Signal transduction via unstructured protein conduits

(2008) A Keith Dunker et al.   Nature Chemical Biology

Suppression of Linear Side Products by Macromolecular Crowding in Nonribosomal Enterobactin Biosynthesis

(2008) Zu-Feng Guo et al.   ORGANIC LETTERS

Mimicking the Plant Cell Interior under Water Stress by Macromolecular Crowding: Disordered Dehydrin Proteins Are Highly Resistant to Structural Collapse

(2008) J.-M. Mouillon et al.   PLANT PHYSIOLOGY

Crowded, cell-like environment induces shape changes in aspherical protein

(2008) D. Homouz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intrinsic disorder in scaffold proteins: Getting more from less

(2008) Marc S. Cortese et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Concerted Action of Metals and Macromolecular Crowding on the Fibrillation of α-Synuclein

(2008) Larissa Munishkina et al.   PROTEIN AND PEPTIDE LETTERS

The effects of macromolecular crowding on the mechanical stability of protein molecules

(2008) Jian-Min Yuan et al.   PROTEIN SCIENCE

BIOCHEMISTRY: Controlled Chaos

(2008) V. N. Uversky et al.   SCIENCE

Fuzzy complexes: polymorphism and structural disorder in protein–protein interactions

(2007) Peter Tompa et al.   TRENDS IN BIOCHEMICAL SCIENCES

Solvent-induced collapse of α-synuclein and acid-denatured cytochrome c

(2002) Artemiza S. Morar et al.   PROTEIN SCIENCE