Article
Engineering, Biomedical
Xue Zhang, Wenbi Wu, Yulan Huang, Xiong Yang, Maling Gou
Summary: The application of 3D bioprinting in the biomedical field is growing, but the lack of bioinks that have both biocompatibility and printability remains a challenge. Silk fibroin materials are promising for biomedical applications due to their good biocompatibility. In this study, an ASF-based bioink was developed for DLP 3D bioprinting. The ASF-MA hydrogel showed mechanical properties, biocompatibility, and improved cell adhesion. Furthermore, the ASF-MA hydrogels promoted cell adhesion, migration, and proliferation. The ASF-MA hydrogels have potential applications in the biomedical field.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Ya Ma, Lian Duan, Jianfeng Sun, Shuangquan Gou, Fengyuan Chen, Yuqi Liang, Fangyin Dai, Bo Xiao
Summary: This study developed engineered Antheraea pernyi silk fibroin nanoparticles loaded with an anti-inflammatory drug, resveratrol, to target colonic epithelial cells and macrophages for effective ulcerative colitis (UC) treatment. The nanoparticles showed lysosomal escape capacity, pH and reactive oxygen species responsiveness, and targeted delivery to the cytoplasm for on-demand drug release. Oral treatment with these nanoparticles relieved UC symptoms by restoring damaged colonic epithelial barriers, reducing inflammation, and rebalancing intestinal microbiota.
Article
Biotechnology & Applied Microbiology
Xueping Liu, Hong Luo, Longxing Niu, Yanfei Feng, Peng Pan, Jicheng Yang, Mingzhong Li
Summary: This study aimed to develop a gene carrier that can effectively deliver therapeutic genes to tumor cells. The PEG-MMP2SSP-modified CASF (CASFMP) showed promising results in inhibiting tumor cell proliferation with lower cytotoxicity to normal cells, making it a potential compound for gene therapy.
Article
Polymer Science
Bo-Xiang Wang, Jia Li, De-Hong Cheng, Yan-Hua Lu, Li Liu
Summary: In this study, novel thermoresponsive hydrogel nanofibers based on Antheraea pernyi silk fibroin (ASF) were successfully fabricated for colon cancer cell culture. The hydrogel nanofibers showed improved cell adhesion, proliferation, and viability compared to traditional hydrogels. These findings suggest that ASF-based nanofibers have potential as an in vitro cancer cell culture platform.
Article
Materials Science, Multidisciplinary
Shengzhi Zou, Suna Fan, Ana L. Oliveira, Xiang Yao, Yaopeng Zhang, Huili Shao
Summary: Combining Antheraea pernyi silk fibroin nanofibers with pure gelatin bioink improves shape fidelity and cytocompatibility in 3D printing, showing promising potential for biomedical applications.
ADVANCED FIBER MATERIALS
(2022)
Article
Engineering, Biomedical
Yajun Shuai, Huan Lu, Ruyin Lv, Jie Wang, Quan Wan, Chuanbin Mao, Mingying Yang
Summary: Using biomacromolecules as templates to control hydroxyapatite crystal nucleation and growth, an artificial periosteum is prepared by biomineralizing Antheraea pernyi fibroin (AF) membrane with prenucleated nanoclusters. This approach promotes osteogenic differentiation of MSCs, induces bone matrix protein formation, and enhances the mechanical properties of the AF membrane. The biomineralized AF membranes show good cytocompatibility, hemocompatibility, and histocompatibility in vitro and in vivo, making them a promising strategy in the field of bone tissue engineering.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Physical
Shan Du, Yunhang Wei, Yuhao Tan, Yong Li, Jingliang Li, Ting Wu, Lu Han, Ming Wang, Shaharia Ahmed, Weitao Zhou, Linqian Wu, Tianhui Zhang
Summary: Silk fibroin from Antheraea pernyi (A. pernyi), rich in Arg-Gly-Asp (RGD) moiety, can self-assemble into mesoporous particles without organic solvents, showing potential for sustained drug release.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Materials Science, Textiles
Hui Yang, Yuhang Guo, Qiang Zhang, Shuqin Yan, Renchuan You
Summary: This study investigated the effects of different degumming methods on the properties of regenerated Antheraea pernyi silk fibroin biomaterials. It was found that silk fibroin prepared by NaHCO3 degumming had a higher molecular weight, leading to improved mechanical properties of ASF film, while Na2CO3 degumming produced more low molecular weight silk fibroin.
JOURNAL OF NATURAL FIBERS
(2022)
Article
Biotechnology & Applied Microbiology
Linling Huang, Yi Jiang, Xueping Liu, Ying Guo, Yanfei Feng, Peng Pan, Mingzhong Li, Yu Liu
Summary: In this study, cationized Antheraea pernyi silk fibroin (CASF) was used to coat adenovirus (Ad) to create CASF/Ad complexes as delivery vectors for vascular endothelial growth factor 165 and angiopoietin-1 dual genes. The results showed that CASF coating reduced the cytotoxicity of Ad and promoted cell proliferation, indicating the potential application of CASF/Ad complexes in promoting vascular network regeneration.
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Anurupa Goswami, Dipali Devi
Summary: This study provides a preliminary characterization of liquid silk from the middle silk gland of a non-mulberry silkworm, and analyzes the sericin protein using in-silico methods. The research demonstrates the conformational patterns of the liquid silk inside the silk gland, and suggests the transitional state of the silk with both crystalline and amorphous contents.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Materials Science, Multidisciplinary
Yu Duan, Xin Chen, Zhengzhong Shao
Summary: The research showed that the regenerated Antheraea pernyi silk fibroin obtained by dissolving in calcium nitrate solution had reduced molecular weight and similar thermal decomposition properties compared to the native Antheraea pernyi silk fibroin. However, the tensile properties of the RASF film were observed to be similar to those of the regenerated Bombyx mori silk fibroin film.
MATERIALS SCIENCE-MEDZIAGOTYRA
(2021)
Article
Chemistry, Multidisciplinary
Boxiang Wang, Hangdan Xu, Jia Li, Dehong Cheng, Yanhua Lu, Li Liu
Summary: In this study, Antheraea pernyi silk fibroin (ASF) and allyl glycidyl ether (AGE) were used to prepare hydrogels with superior cytocompatibility. The hydrogels demonstrated good thermoresponsive and degradability without any crosslinking agent, and showed excellent cell proliferation and cytocompatibility for b End.3 cells. These degradable and thermoresponsive ASF based hydrogels may have potential applications in cell delivery devices and tissue engineering.
Article
Polymer Science
Jia Li, Bo-Xiang Wang, De-Hong Cheng, Yan-Hua Lu
Summary: The Antheraea Pernyi silk gland protein has been synthesized by tussah silk glands and shows great potential as a biomaterial due to its excellent biocompatibility. In this study, drug-loaded bio-hydrogels based on Antheraea Pernyi silk gland protein were fabricated using an eco-friendly method. The results demonstrate the porous structure and high drug release capability of the bio-hydrogels, as well as their non-toxic and pro-proliferative effects on cultured cells.
Article
Polymer Science
Beste Cagdas Tunali, Omer Akturk, Durukan Sahingoz, Mustafa Turk, Ayten Celebi Keskin
Summary: In this study, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized using the pulsed laser ablation in liquid (PLAL) technique, and then combined with Bombyx mori silk fibroin (SF) to form nanocomposites (PEI-SF-AuNPs). The biocompatibility of PEI-AuNPs and PEI-SF-AuNPs in breast cancer cells MCF-7 and MDA-MB231 were tested, and it was found that PEI-AuNP1 showed the least cytotoxicity. Therefore, PEI-AuNP1 nanocomposites can be suggested as siRNA carriers in gene-based breast cancer therapies.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Lihe Cao, Wenping Tian, Baocheng Li, Fuchao You, Ji Hang, Duan Hao, Donghuang He
Summary: This research discussed the value of regenerated wild Antheraea pernyi silk fibroin (RWSF)/polyvinyl alcohol (PVA) nanofiber scaffold (NFS) in repairing the calcaneal tendon defect (CTD). The results showed that RWSF/PVA NFS exhibited good biocompatibility, promoting collagen secretion while inhibiting inflammatory factors, thus showing high value in repairing CTD.
SCIENCE OF ADVANCED MATERIALS
(2023)