Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
A-Yeung Jang, Min-Joo Choi, Yong Zhi, Hyun-Jung Ji, Ji-Yun Noh, Jin-Gu Yoon, Hee-Jin Cheong, Woo-Joo Kim, Ho-Seong Seo, Joon-Young Song
Summary: The standardized GBS-ELISA developed in this study shows high specificity and accuracy in evaluating the immunogenicity of GBS vaccines against the three main serotypes. The results demonstrate good performance of the ELISA in assessing the three serotypes, aiding in the development and evaluation of GBS vaccines.
Article
Medicine, General & Internal
Lotfi S. Bin Dahman, Khalid M. Sumaily, Essa M. Sabi, Mohammed A. Hassan, Abeer M. Bin Thalab, Asrar S. Sayad, Saleh M. Bin Kolaib, Fatima M. Alhadhrmi
Summary: This study compared the serum ferritin levels measured by ELISA, Cobas e411, and Cobas Integra 400 methods. The results showed no significant difference between ELISA and Cobas e411, while the values from Cobas Integra 400 were significantly higher. Cobas e411 and Cobas Integra 400 methods demonstrated higher sensitivity, specificity, and accuracy. Further investigations with larger samples are needed to determine interchangeability.
Article
Biochemical Research Methods
Tetsuya Saita, Hiroto Kataoka, Rintaro Sogawa, Tadashi Hayama, Ryoko Tomita, Akira Monji, Yoshito Mizoguchi, Chisato Shimanoe
Summary: The study successfully developed a simple and specific ELISA method for the quantification of PEA, showing comparable results to HPLC measurements and a strong correlation between the values determined by the two methods.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Chemistry, Analytical
Jian Zhou, Jie Liu, Shanshan Song, Liqiang Liu, Chuanlai Xu, Hua Kuang, Wei Ma
Summary: A sensitive and specific monoclonal antibody was developed to detect Diuron in water and sugarcane samples. An immunochromatographic assay strip based on this antibody showed visual detection limits and critical values for Diuron in water and sugarcane samples, which were validated using HPLC-MS.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Applied
Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang
Summary: This research explores a novel strategy to enhance the catalytic activity of enzymes, leading to the development of a sensitive ELISA method. By utilizing a chemical staining strategy, the catalytic activity of horseradish peroxidase was directly improved, and a new CS-ELISA method for detecting clenbuterol was established. The CS-ELISA exhibited a 2-fold improvement in sensitivity and linear range compared to conventional ELISA, and demonstrated good applicability in detecting clenbuterol in pork tenderloin samples.
Article
Chemistry, Analytical
Ying Wang, Tong Bu, Yuanyuan Cao, Haiyu Wu, Jia Xi, Qinlin Feng, Chenyu Xuan, Li Wang
Summary: In this study, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed using the bimetallic PdRu nanozyme as a catalytic carrier for detecting Escherichia coli O157:H7. The PdRu nanozyme exhibited high catalytic activity and affinity with antibody, ensuring successful establishment of a novel colorimetric biosensor. The PdRu-based ELISA achieved ultrasensitive detection sensitivity and satisfactory specificity and reproducibility, making it suitable for bioassays and clinical diagnostics.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Yesenia Castillo, Melissa T. Wardle, Ricardo Gamboa, Mayra Elizalde, Percy Vilchez, Silvia Rodriguez, Robert H. Gilman, Armando E. Gonzalez, Seth E. O'Neal, Hector H. Garcia, Cysticercosis Working Grp Peru
Summary: Coproantigen detection by enzyme-linked immunosorbent assay (coAg ELISA) is a vital tool for detecting and treating cases of Taenia solium taeniasis. However, the assay's procedures require costly materials and sophisticated equipment, which are typically inaccessible in rural settings where the disease is endemic.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Guo-Yong Liu, Yu Shi, Jian Sun, Jing Mu
Summary: Enzyme linked immunosorbent assay (ELISA) plays a crucial role in environmental monitoring, food safety testing, and medical diagnosis due to its high-throughput, easy reading, simple operation, and low cost. However, the conventional ELISA suffers from a high detection limit, limiting its application in analysis and detection. To overcome this issue, various fluorescent materials have been developed, such as organic small molecules, silicon/carbon nanoparticles, metal nanoclusters, and quantum dots, to construct ELISA with improved performance. Different labeling enzymes, including alkaline phosphatase and horseradish peroxidase, have also been reviewed. The prospects of ELISA based on different fluorescent materials are discussed.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Orthopedics
Sean W. Flannery, Ata M. Kiapour, David J. Edgar, Martha M. Murray, Jillian E. Beveridge, Braden C. Fleming
Summary: This study validated a deep learning model for automatic segmentation of repaired and reconstructed anterior cruciate ligaments, showing decreased anatomical performance compared to intact ACLs but no significant differences in quantitative features.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Orthopedics
Naga Padmini Karamchedu, Braden C. Fleming, Benedikt L. Proffen, Nicholas J. Sant, Gabriela Portilla, Lauren R. Parola, Janine Molino, Martha M. Murray
Summary: The study aimed to investigate the effect of ECM-B composite on posttraumatic osteoarthritis after ACL injury and found that the composite made with aseptically processed ECM could mitigate the gait and histologic changes associated with osteoarthritis. However, caution must be taken in selecting a terminal sterilization method as it may affect the treatment effectiveness.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Orthopedics
Sean W. Flannery, Dominique A. Barnes, Meggin Q. Costa, Danilo Menghini, Ata M. Kiapour, Edward G. Walsh, Dennis E. Kramer, Martha M. Murray, Braden C. Fleming
Summary: The study applies transfer learning to automate the segmentation and evaluation of collagen organization in ACL using T-2* mapping. Results show that the performance of T-2* segmentation is comparable to CISS, and the automated segmentation outperforms independent manual segmentation and performs equally well as retest segmentation.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Orthopedics
Sean W. Flannery, Edward G. Walsh, Ryan M. Sanborn, Cynthia A. Chrostek, Meggin Q. Costa, Shankar G. Kaushal, Martha M. Murray, Braden C. Fleming, Ata M. Kiapour
Summary: This study aimed to assess the reproducibility of T-2* relaxometry and CISS signal across different scanners without changing hardware-necessitated parameters. The results suggest that small differences in acquisition parameters can lead to significant discrepancies in T-2* and CISS values across scanners, but these differences can be mitigated through rescaling the signal intensity distribution.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Review
Cell Biology
Yajun Liu, Jonah Schwam, Qian Chen
Summary: Aging is a process of functional decline and tissue degeneration that occurs as organisms age. Cellular senescence, caused by replicative stress, telomere erosion, and stem cell exhaustion, plays a key role in tissue degradation and cell death in aging-related diseases. The interaction between senescent and non-senescent cells may lead to the death of non-senescent cells and tissue degeneration in aging organs.
Article
Orthopedics
Megan P. Pinette, Janine Molino, Benedikt L. Proffen, Martha M. Murray, Braden C. Fleming
Summary: This study compared the macroscopic cartilage damage in male and female Yucatan minipigs after ACL surgery, and found that regardless of treatment, females had significantly worse cartilage damage scores, especially in the trochlear region.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Orthopedics
Danilo Menghini, Shankar G. Kaushal, Sean W. Flannery, Kirsten Ecklund, Martha M. Murray, Braden C. Fleming, Ata M. Kiapour
Summary: Smaller ACL size in females is believed to contribute to a higher incidence of ACL tears. This study used MRI to quantify the CSA of the ACL and found different patterns of CSA changes in males and females. Males had a significantly larger CSA only in the distal portion of the ACL, while in females, ACL CSA was associated with intercondylar notch width.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Jonah Donnenfield, Naga Padmini Karamchedu, Benedikt L. Proffen, Janine Molino, Martha M. Murray, Braden C. Fleming
Summary: This preclinical study examines the relationship between inflammatory response and cartilage damage following joint injury. The study finds that the presence of inflammatory cytokines in synovial fluid is associated with better cartilage outcomes, while higher loading on the injured leg and contralateral leg are associated with worse cartilage outcomes. This research is important for further understanding the mechanisms of cartilage pathology after joint injury.
Article
Orthopedics
Meggin Q. Costa, Gary J. Badger, Cynthia A. Chrostek, Orianna D. Carvalho, Stacy L. Faiola, Paul D. Fadale, Michael J. Hulstyn, Holly C. Gil, Robert M. Shalvoy, Braden C. Fleming
Summary: This study aimed to evaluate the effect of initial graft tension and patient sex on posttraumatic osteoarthritis (PTOA) outcomes 10 to 12 years after ACL reconstruction (ACLR). The results showed that ACLR failed to prevent PTOA regardless of initial graft tension. However, male patients treated with a low initial graft tension may be at greater risk for PTOA.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Orthopedics
Ryan M. Sanborn, Gary J. Badger, Braden C. Fleming, Ata M. Kiapour, Paul D. Fadale, Michael J. Hulstyn, Brett D. Owens, Benedikt Proffen, Nicholas Sant, Gabriela Portilla, Christina Freiberger, Rachael Henderson, Samuel Barnett, Meggin Costa, Cynthia Chrostek, Kirsten Ecklund, Lyle J. Micheli, Martha M. Murray, Yi-Meng Yen, Dennis E. Kramer
Summary: Younger age and higher medial tibial slope (MTS) are predictors of ipsilateral ACL revision surgery after the bridge-enhanced ACL restoration (BEAR) procedure. Younger patients with higher tibial slopes should be aware of the increased risk for revision surgery when deciding to undergo ACL restoration.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2023)
Article
Multidisciplinary Sciences
Lauren R. Parola, Megan P. Pinette, Benedikt L. Proffen, Nicholas J. Sant, N. Padmini Karamchedu, Meggin Q. Costa, Janine Molino, Braden C. Fleming, Martha M. Murray
Summary: This study aimed to investigate the effects of intraarticular injections of an extracellular matrix (ECM) powder and blood composite (ECM-B) on post-operative gait parameters. The results showed that ECM-B could improve gait for a limited time without causing adverse cartilage changes or severe lymphatic reactions in an osteoarthritis (OA) model.
Article
Multidisciplinary Sciences
Jonah I. Donnenfield, Naga Padmini Karamchedu, Benedikt L. Proffen, Janine Molino, Braden C. Fleming, Martha M. Murray
Summary: In order to study the transcriptomic changes during early- to mid-stage posttraumatic osteoarthritis (PTOA) development, 72 Yucatan minipigs underwent transection of the anterior cruciate ligament. The subjects were divided into three groups: no further intervention, ligament reconstruction, or ligament repair. The gene expression analysis showed that there was an initial increase in transcriptomic differences at 1 and 4 weeks followed by a stark reduction at 52 weeks. Different treatments were found to genetically modulate the course of PTOA. Specific genes were identified as being upregulated in the cartilage of injured subjects across all timepoints, and certain genes that have not been associated with PTOA before were found to be differentially expressed at the 52-week timepoint.
Article
Biotechnology & Applied Microbiology
Jonah I. Donnenfield, Benedikt L. Proffen, Braden C. Fleming, Martha M. Murray
Summary: The relationship between cartilage and synovium in osteoarthritis research has been studied, but not in mid-stage disease development. This study compared the gene expression profiles of cartilage and synovium in a large animal model of posttraumatic osteoarthritis. The results showed that cartilage had upregulated genes related to immune activation, while synovium had upregulated genes related to Wnt signaling. Furthermore, the use of an extracellular matrix scaffold in surgery showed potential chondroprotective effects through activating ion homeostasis and tissue remodeling pathways in cartilage.
BIOENGINEERING-BASEL
(2023)
Review
Chemistry, Medicinal
Brandon Vorrius, Zhen Qiao, Jonathan Ge, Qian Chen
Summary: The musculoskeletal system is composed of specialized connective tissues that provide protection, structure, and mobility to the body. However, diseases of the musculoskeletal system are increasing in burden, resulting in pain, disability, and a decrease in quality of life. Regenerative drug therapies for musculoskeletal disorders are in demand, but effective drug delivery poses challenges.
Article
Biotechnology & Applied Microbiology
Payam Zandiyeh, Lauren R. Parola, Meggin Q. Costa, Madalyn J. Hague, Janine Molino, Braden C. Fleming, Jillian E. Beveridge
Summary: Using EMG signal analysis technique and machine learning approach, the study found that muscle activity patterns in ACLR patients were different from healthy controls, and smaller ipsilateral thigh muscle girth was the strongest predictor of MRI scores.
BIOENGINEERING-BASEL
(2023)