Article
Biochemistry & Molecular Biology
Joao Filipe Neves, Olivia Petrvalska, Francesco Bosica, Francois-Xavier Cantrelle, Hamida Merzougui, Gavin O'Mahony, Xavier Hanoulle, Tomas Obsil, Isabelle Landrieu
Summary: Protein-protein interactions (PPIs) involving 14-3-3 proteins and Tau are potential targets for Alzheimer's disease treatment. Phosphorylation of Tau by PKA is crucial for the formation of a stable complex with 14-3-3 sigma, with an affinity in the 1-10 micromolar range. Each monomer of the 14-3-3 sigma dimer binds to two different phosphorylated peptides of Tau-PKA, suggesting a 2:1 stoichiometry for 14-3-3/Tau-PKA.
Article
Endocrinology & Metabolism
Ying-Xiang Huo, Wei Wei, Yang Liu, Ya-Nan Ma, Jun-Min Tao, Ning-Ning Wang, Xiao-Feng Li, Xin Chen
Summary: The study found a U-shaped correlation between serum cystatin C levels and obesity risk in boys, with higher levels associated with an increased risk, while in girls, the risk of obesity initially increased and then decreased with increasing cystatin C levels. Longitudinal studies are needed to further investigate the diagnostic potential of cystatin C in early obesity progression in adolescents of different sexes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Thilini M. Wijerathna, Nicholas A. Buckley, Indika B. Gawarammana, Jacques Raubenheimer, Seyed Shahmy, Umesh Chathuranga, Chathura Palangasinghe, Fathima Shihana, Fahim Mohamed
Summary: This study investigates the epidemiology and clinical outcomes of MCPA poisoning in Sri Lanka between 2002 and 2019, and analyzes the acute kidney injury in a subset of patients. The results show that MCPA poisoning can cause renal injury, but severe injury is uncommon. Patient age and ingested dose are associated with the severity of the condition, and most patients with renal dysfunction have no pre-existing kidney diseases.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
S. Grace Herod, Annie Dyatel, Stefanie Hodapp, Marko Jovanovic, Luke E. Berchowitz
Summary: Amyloids play important roles in age-related diseases. Some cells use reversible amyloid-like structures for translational control. Yeast 14-3-3 proteins bind to amyloid-like assemblies and facilitate their clearance, contributing to global protein aggregate homeostasis. These findings suggest that 14-3-3 proteins may protect against pathological protein aggregates.
Article
Immunology
Meng Li, Hongping Chen, Pengqi Yin, Jihe Song, Fangchao Jiang, Zhanbin Tang, Xuehui Fan, Chen Xu, Yingju Wang, Yang Xue, Baichao Han, Haining Wang, Guozhong Li, Di Zhong
Summary: This study explored the biomarkers of multiple sclerosis (MS) using bioinformatics methods and identified key extracellular proteins such as IL17A, IL2, CD44, and IGF1. Del-1 was suggested as a potential biomarker of MS, with its levels associated with the development and survival of MS patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Claire C. Munier, Christian Ottmann, Matthew W. D. Perry
Summary: The 14-3-3 proteins play crucial roles in regulating the inflammatory response at genetic, molecular, and cellular levels. They affect key components of the immune response and can lead to clinical syndromes when their recognition processes are disrupted. Abnormal levels of 14-3-3 contribute to undesirable immune responses and chronic inflammatory conditions.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Dan Li, Linlu Zhao, Jin Qian, Heng Liu, Jinmao You, Ziyi Cheng, Fabiao Yu
Summary: In this paper, a surface-enhanced Raman scattering-based aptasensor is developed for duplex detection of protein biomarkers in acute kidney injury (AKI). The sensor utilizes the base-pairing specificity of nucleic acids to form a self-assembled structure and increases the Raman scattering signal through the release of gold nanoparticles, achieving high sensitivity analysis. Experimental results confirm the general applicability of this sensor in early diagnosis of AKI.
Article
Parasitology
Patricia Ruiz, Angela Duran, Francisco Javier Duque, Mario Alberto Gonzalez, Jose Ignacio Cristobal, Paloma Nicolas, Eva Maria Perez-Merino, Beatriz Macias-Garcia, Rafael Barrera
Summary: Canine leishmaniasis (CanL) is a disease caused by Leishmania infantum that can range from mild to severe. Dogs with CanL often have renal dysfunction, but traditional biomarkers are not accurate enough. This study aimed to investigate the performance of urinary cystatin C (CysC) and N-acetyl-beta-D-glucosaminidase (NAG) as biomarkers for renal damage in different stages of CanL. The results showed that both uCysCc and uNAGc increased significantly from the LI stage, and a cutoff value was determined for early detection of renal damage.
VETERINARY PARASITOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu-Qing Liu, Chu-Qiao Liang, Zhi-Wei Chen, Jun Hu, Jin-Jian Hu, Yun-Yi Luo, Yong-Xiang Chen, Yan-Mei Li
Summary: 14-3-3C participates in regulating Tau liquid-liquid phase separation, stabilizes Tau droplets, inhibits Tau droplet-driven tubulin assembly, and disrupts the liquid-liquid phase separation of phosphorylated and glycated Tau, thereby inhibiting amyloid aggregation.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Bente A. Somsen, Fenna W. B. Craenmehr, Wei-Hong W. Liu, Auke A. Koops, Marloes A. M. Pennings, Emira J. Visser, Christian Ottmann, Peter J. Cossar, Luc Brunsveld
Summary: Molecular glues represent a novel approach in drug discovery, however, the targeted stabilization of protein complexes is still challenging due to a lack of drug design rules. This study demonstrates the successful development of a peptide-based molecular glue that selectively stabilizes the 14-3-3/ChREBP protein-protein interaction by utilizing the functional mapping of hotspots.
Article
Medicine, General & Internal
Agnieszka Kulczynska-Przybik, Maciej Dulewicz, Julia Doroszkiewicz, Renata Borawska, Ala Litman-Zawadzka, Daria Arslan, Alina Kulakowska, Jan Kochanowicz, Barbara Mroczko
Summary: The CSF levels of NfL, RTN-4, and tau at the time of diagnosis could potentially serve as diagnostic markers for multiple sclerosis, with NfL showing the best clinical value.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Transplantation
Euan N. Paterson, Alexander P. Maxwell, Frank Kee, Sharon Cruise, Ian S. Young, Bernadette McGuinness, Gareth J. McKay
Summary: This study found a significant association between decreased eGFR and increased risk of cognitive impairment, especially when calculated using Cys-C. In addition, CKD Stages 3-5 were also associated with an increased risk of cognitive impairment.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2021)
Article
Clinical Neurology
Yahui Zhu, Yunyun Huo, Jiongming Bai, Mao Li, Hongfen Wang, Jiao Wang, Xusheng Huang
Summary: This study aimed to investigate the relationship between serum Cystatin C (CysC) and survival in ALS patients. The results showed that ALS patients with high CysC levels had shorter median survival than those with lower CysC levels, and serum CysC level might be an independent predictor of ALS survival.
NEUROLOGICAL SCIENCES
(2023)
Article
Clinical Neurology
Xin Lin, Yuanhao Yang, Melissa Gresle, Gabriel Cuellar-Partida, Xikun Han, Jim Stankovich, Steve Simpson-Yap, Valery Fuh-Ngwa, Jac Charlesworth, Kathryn P. Burdon, Helmut Butzkueven, Bruce Taylor, Yuan Zhou
Summary: This study identifies 39 novel plasma and brain proteins associated with multiple sclerosis onset risk and assesses their expression at tissue and cell level. Almost two-thirds of the identified candidates were also associated with disability progression.
Article
Biochemistry & Molecular Biology
Bin Bai, Tao Wang, Xiaonan Zhang, Xinlei Ba, Na Zhang, Yanjiao Zhao, Xipeng Wang, Yang Yu, Bing Wang
Summary: In this study, it was found that the protein tyrosine phosphatase PTPN22 inhibits T cell activation by dephosphorylating essential proteins in the T cell receptor-mediated signalling pathway. PTPN22 binds to 14-3-3 tau protein and leads to its dephosphorylation, affecting the activity of the PI3K pathway and the expression of inflammatory factors.
Article
Immunology
Andrea Pilotto, Stefano Masciocchi, Irene Volonghi, Massimo Crabbio, Eugenio Magni, Valeria De Giuli, Francesca Caprioli, Nicola Rifino, Maria Sessa, Michele Gennuso, Maria Sofia Cotelli, Marinella Turla, Ubaldo Balducci, Sara Mariotto, Sergio Ferrari, Alfonso Ciccone, Fabrizio Fiacco, Alberto Imarisio, Barbara Risi, Alberto Benussi, Enrico Premi, Emanuele Foca, Francesca Caccuri, Matilde Leonardi, Roberto Gasparotti, Francesco Castelli, Gianluigi Zanusso, Alessandro Pezzini, Alessandro Padovani
Summary: SARS-CoV-2 infection is associated with a wide spectrum of encephalitis characterized by different clinical presentations, response to treatment, and outcomes. The study identified subtypes of encephalitis based on MRI results, with patients showing acute demyelinating encephalomyelitis and limbic encephalitis having a delayed onset and more severe COVID-19 respiratory involvement. Patients with MRI alterations exhibited worse response to treatment and final outcomes.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Letter
Clinical Neurology
Andrea Pilotto, Alberto Benussi, Ilenia Libri, Stefano Masciocchi, Loris Poli, Enrico Premi, Antonella Alberici, Enrico Baldelli, Sonia Bonacina, Laura Brambilla, Matteo Benini, Salvatore Caratozzolo, Matteo Cortinovis, Angelo Costa, Stefano Cotti Piccinelli, Elisabetta Cottini, Viviana Cristillo, Ilenia Delrio, Massimiliano Filosto, Massimo Gamba, Stefano Gazzina, Nicola Gilberti, Stefano Gipponi, Marcello Giunta, Alberto Imarisio, Paolo Liberini, Martina Locatelli, Francesca Schiano, Ranata Rao, Barbara Risi, Luca Rozzini, Andrea Scalvini, Veronica Vergani, Irene Volonghi, Nicola Zoppi, Barbara Borroni, Mauro Magoni, Matilde Leonardi, Gianluigi Zanusso, Sergio Ferrari, Sara Mariotto, Alessandro Pezzini, Roberto Gasparotti, Ciro Paolillo, Alessandro Padovani
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2021)
Article
Immunology
Andrea Pilotto, Stefano Masciocchi, Irene Volonghi, Valeria De Giuli, Francesca Caprioli, Sara Mariotto, Sergio Ferrari, Silvia Bozzetti, Alberto Imarisio, Barbara Risi, Enrico Premi, Alberto Benussi, Emanuele Foca, Francesco Castelli, Gianluigi Zanusso, Salvatore Monaco, Paola Stefanelli, Roberto Gasparotti, Anastasia Zekeridou, Andrew McKeon, Nicholas J. Ashton, Kaj BlennoW, Henrik Zetterberg, Alessandro Padovani
Summary: SARS-CoV-2-related encephalitis is associated with prominent glial activation and neuroinflammatory markers, with neuronal markers increased only in severe cases. The pattern of CSF alterations suggests cytokine-release syndrome as the main inflammatory mechanism of SARS-CoV-2-related encephalitis.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Clinical Neurology
Maria Di Gregorio, Valentina Liliana Adriana Torri Clerici, Giuseppe Fenu, Lorenzo Gaetani, Antonio Gallo, Paola Cavalla, Paolo Ragonese, Pietro Annovazzi, Alberto Gajofatto, Luca Prosperini, Doriana Landi, Carolina Gabri Nicoletti, Caterina Di Carmine, Rocco Totaro, Viviana Nociti, Chiara De Fino, Diana Ferraro, Valentina Tomassini, Carla Tortorella, Isabella Righini, Maria Pia Amato, Alessia Manni, Damiano Paolicelli, Pietro Iaffaldano, Roberta Lanzillo, Marcello Moccia, Fabio Buttari, Roberta Fantozzi, Raffaella Cerqua, Sara Zagaglia, Deborah Farina, Giovanna De Luca, Maria Chiara Buscarinu, Federica Pinardi, Eleonora Cocco, Claudio Gasperini, Claudio Marcello Solaro, Massimiliano Di Filippo
Summary: The study found that tumefactive multiple sclerosis (TuMS) primarily affects women with a young onset age, and presents with unique disease characteristics. Some patients require multiple acute-phase treatments, while those with certain risk factors may experience disease progression.
EUROPEAN JOURNAL OF NEUROLOGY
(2021)
Review
Neurosciences
Matteo Tagliapietra, Emma Frasson, Davide Cardellini, Sara Mariotto, Sergio Ferrari, Gianluigi Zanusso, Mauro Plebani, Salvatore Monaco
Summary: Anti-IgLON5 disease is a rare neurodegenerative tauopathy with varied clinical spectrum, presenting symptoms including sleep disorders, bulbar dysfunction, and gait abnormalities. While conventional brain MRI may be unrevealing, CSF findings and immunotherapy responses can be helpful in diagnosis and treatment for some patients.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Review
Clinical Neurology
Peter Hermann, Brian Appleby, Jean-Philippe Brandel, Byron Caughey, Steven Collins, Michael D. Geschwind, Alison Green, Stephane Haik, Gabor G. Kovacs, Anna Ladogana, Franc Llorens, Simon Mead, Noriyuki Nishida, Suvankar Pal, Piero Parchi, Maurizio Pocchiari, Katsuya Satoh, Gianluigi Zanusso, Inga Zerr
Summary: Sporadic Creutzfeldt-Jakob disease is a fatal neurodegenerative disease caused by misfolded prion proteins. Its diagnosis relies on a combination of neuropsychiatric symptoms, CSF markers, and imaging. The development of assays like RT-QuIC has improved diagnostic accuracy.
Article
Clinical Neurology
Matteo Tagliapietra, Davide Cardellini, Moreno Ferrarini, Silvia Testi, Sergio Ferrari, Salvatore Monaco, Tiziana Cavallaro, Gian Maria Fabrizi
Summary: The study highlights the frequent occurrence of the RFC1 AAGGG repeat expansion in patients diagnosed with CIAP and characterizes the clinical and pathological features associated with this neuro(no)pathy. The mutation is associated with sensory ataxia, autonomic disturbances, and retained deep tendon reflexes.
JOURNAL OF NEUROLOGY
(2021)
Letter
Clinical Neurology
Sara Mariotto, Matteo Gastaldi, Luisa Grazian, Chiara Mancinelli, Ruggero Capra, Romain Marignier, Daniela Alberti, Serena Zanzoni, Kathrin Schanda, Diego Franciotta, Francesca Calabria, Salvatore Monaco, Markus Reindl, Sergio Ferrari, Alberto Gajofatto
Summary: In patients with MOGAD, levels of neurofilament light chain (NfL) indicate that the primary axonal damage occurs at disease onset, which may be the main driver of long-term disability. Follow-up measurements showed stable or decreased NfL levels, providing important insights into the progression of the disease and potential treatment implications.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2021)
Meeting Abstract
Clinical Neurology
Andrea Pilotto, Stefano Masciocchi, Elisabetta Del Zotto, Irene Volonghi, Eugenio Magni, Valeria De Giuli, Bruno Censori, Nicola Rifino, Maria Sessa, Michele Gennuso, Sara Mariotto, Sergio Ferrari, Lucia Tesolin, Franco Teatini, Maria Sofia Cotelli, Marinella Turla, Ubaldo Balducci, Raphael Bernard-Valnet, Alfonso Ciccone, Fabrizio Fiacco, Alberto Imarisio, Barbara Risi, Emanuele Foca, Arnaldo Caruso, Matilde Leonardi, Roberto Gasparotti, Francesco Castelli, Gianluigi Zanusso, Alberto Romagnolo, Leonardo Lopiano, Piergiorgio Lochner, Klaus Fassbender, Alessandro Padovani
JOURNAL OF THE NEUROLOGICAL SCIENCES
(2021)
Meeting Abstract
Clinical Neurology
Maurizio Gallucci, Anna Paola Mazzarolo, Vittorio Fiore, Matteo Bendini, Gianluigi Zanusso, Laura Bonanni
JOURNAL OF THE NEUROLOGICAL SCIENCES
(2021)
Article
Clinical Neurology
Maria Paola Cecchini, Lorenzo Brozzetti, Nicolo Cardobi, Luca Sacchetto, Davide Gibellini, Stefania Montemezzi, Marta Cheli, Paolo Manganotti, Salvatore Monaco, Gianluigi Zanusso
Summary: A 17-year-old female patient experienced long-lasting anosmia after a mild infection, with partial recovery 15 months later. Neuroimaging did not show microvascular injury in olfactory bulbs and brain, and the patient exhibited symptoms of anosmia with parosmia phenomena and gustatory impairment. Immunocytochemistry of olfactory mucosa swabs revealed high expression of ACE2 in sustentacular cells and lower expression in neuronal-shaped cells. Further longitudinal studies are needed to investigate the persistence of chemosensory impairment.
NEUROLOGICAL SCIENCES
(2022)
Article
Clinical Neurology
Alberto Gajofatto, Nicolo Cardobi, Francesca Gobbin, Massimiliano Calabrese, Marco Turatti, Maria Donata Benedetti
Summary: This study used resting state functional magnetic resonance imaging (fMRI) to investigate changes in brain network connectivity in multiple sclerosis (MS) patients treated with Sativex (R). The results showed that Sativex (R) treatment was associated with an increase in brain connectivity in MS patients, particularly in responders.
Article
Food Science & Technology
Antonella Costa, Vincenzina Alio, Sonia Sciortino, Luisa Nicastro, Monica Cangini, Fiorella Pino, Irene Servadei, Angelo La Vignera, Gianluca Fortino, Salvatore Monaco, Sonia Dall'Ara
Summary: Marine biotoxins can be transferred to humans through filter-feeders bivalve molluscs, potentially causing health issues. Harmful Algal Blooms may result in economic losses for aquaculture.
ITALIAN JOURNAL OF FOOD SAFETY
(2021)
Article
Medicine, General & Internal
Emma Frasson, Marco Simonetto, Laura Bertolasi, Giorgio Caneve, Cristina Vilotti, Giampietro Ruzza, Anna Perelli, Maria Grazia Piccinno, Salvatore Monaco
Summary: The patient had previously been treated with statins and then developed necrotizing autoimmune myopathy and myasthenia gravis, posing an unprecedented clinical challenge.
CLINICAL CASE REPORTS
(2021)
Article
Clinical Neurology
Daniela Perra, Matilde Bongianni, Giovanni Novi, Francesco Janes, Valentina Bessi, Stefano Capaldi, Luca Sacchetto, Matteo Tagliapietra, Guido Schenone, Silvia Morbelli, Michele Fiorini, Tatiana Cattaruzza, Giulia Mazzon, Christina D. Orru, Mauro Catalan, Paola Polverino, Andrea Bernardini, Gaia Pellitteri, Mariarosa Valente, Claudio Bertolotti, Benedetta Nacmias, Giandomenico Maggiore, Tiziana Cavallaro, Paolo Manganotti, Gianluigi Gigli, Salvatore Monaco, Flavio Nobili, Gianluigi Zanusso
Summary: The study examined the diagnostic accuracy of alpha-synuclein real-time quaking-induced conversion assay in detecting alpha-synuclein in olfactory mucosa and CSF of patients with suspected dementia with Lewy bodies. The results suggest that this approach could optimize clinical diagnosis and potentially improve concordance with clinical diagnosis.
BRAIN COMMUNICATIONS
(2021)
