Article
Plant Sciences
Marcin Delijewski, Khaled Radad, Christopher Krewenka, Barbara Kranner, Rudolf Moldzio
Summary: Nicotine shows neuroprotective effects on dopaminergic neurons, increasing neurite length and count, as well as affecting glutathione levels.
Article
Biochemistry & Molecular Biology
Guo Wen, Zhongyi Xie, Yao Yang, Yuxue Yang, Qigao Guo, Guolu Liang, Jiangbo Dang
Summary: In this study, researchers identified a gene called NpPP2-B10 that plays a crucial role in resistant tobacco plants. By transferring this gene into susceptible tobacco cultivars, they found that it promotes resistance to black shank disease. Further investigation showed that NpPP2-B10 is involved in the plant immune response through the ubiquitin protease pathway. These findings provide important insights into the mechanisms of tobacco resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Ken-ichi Kurotani, Hideki Hirakawa, Kenta Shirasawa, Yasuhiro Tanizawa, Yasukazu Nakamura, Sachiko Isobe, Michitaka Notaguchi
Summary: Nicotiana benthamiana is a widely used model plant for dicotyledonous angiosperms, but its previous reference genome was fragmented and hindered molecular genetic analysis. However, with improved genome assembly and annotation, N. benthamiana would become more accessible for research purposes.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Denis V. Beliaev, Dmitry V. Tereshonok, Nina F. Lunkova, Ekaterina N. Baranova, Ekaterina S. Osipova, Stepan V. Lisovskii, Galina N. Raldugina, Vladimir V. Kuznetsov
Summary: Cytochrome c3 (uranyl reductase) from Desulfovibrio vulgaris can reduce uranium in bacterial cells and cell-free systems. When this gene was introduced into tobacco under the control of the RbcS promoter, resulting transgenic plants accumulated uranium and showed normal phenotype even in the presence of sublethal concentrations of uranyl ions, unlike control plants. This study discusses the oxidation state of uranium in transgenic plants and potential uses of uranium hyperaccumulation by plants for environmental cleanup.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Gernot Beihammer, Julia Koenig-Beihammer, Benjamin Kogelmann, Valentina Ruocco, Clemens Gruenwald-Gruber, Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Pooja Saxena, Johannes S. S. Gach, Herta Steinkellner, Richard Strasser
Summary: By testing a single-subunit oligosaccharyltransferase from Leishmania donovani (LdOST), we found that it can significantly increase the Fc glycosylation level of IgG1 without changing the overall N-glycan composition. This finding is important for reducing the heterogeneity of plant-produced antibodies and improving their stability and effector functions.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Soyeon Seo, Yumi Kim, Kyyoung Park
Summary: Chloroplasts play important roles in stress responses, regulated by nuclear gene expression through changes in cellular redox state. NPR1, a redox-sensitive transcriptional coactivator, was consistently found in tobacco chloroplasts. It was observed that chloroplast-targeted NPR1 is likely translocated from chloroplasts to the nucleus after processing in the stroma. Translation in the chloroplast is essential for the accumulation of nuclear NPR1 and the expression of stress-related nuclear genes. In addition, chloroplast NPR1 acts as a retrograde signaling that enhances plant adaptability to adverse environments.
Article
Microbiology
Baoyu He, Xiaoyulong Chen, Hong Yang, Tomislav Cernava
Summary: This study investigated the impact of diet on the microbiome of M. persicae, finding significant changes in the bacterial community composition when aphids were transferred to different Solanaceae plants. Notably, there was a substantial increase in Pseudomonas abundance, especially under pepper plant diet. Additionally, negative effects on aphid population dynamics were observed when they were fed on pepper plants.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agronomy
Gabriel Zilnik, Dylan A. Kraus, Hannah J. Burrack
Summary: The study found that soil applications of chlorantraniliprole are not effective for controlling early season tobacco budworm in the field and showed differences in control efficacy between the phytotron and field experiments.
Article
Environmental Sciences
Fatemeh Rajabi, Jenny Jessat, Jawaharlal Nehru Garimella, Frank Bok, Robin Steudtner, Thorsten Stumpf, Susanne Sachs
Summary: This study investigated the physiological and cellular responses of tobacco BY-2 cells to uranium as an abiotic stressor, revealing the interference of uranium with essential element homeostasis and its interaction with Fe deficiency in cultured cells. The results showed time- and concentration-dependent kinetics of uranium in BY-2 cells, as well as changes in uranium speciation in the culture media under different iron conditions. The toxicity of uranium in BY-2 cells was found to be highly dependent on the existence of other micro-macro elements, suggesting negative synergistic effects of uranium and iron on cell viability.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Plant Sciences
Valentina Ruocco, Ulrike Vavra, Julia Koenig-Beihammer, Omayra C. Bolanos-Martinez, Somanath Kallolimath, Daniel Maresch, Clemens Gruenwald-Gruber, Richard Strasser
Summary: Subunit vaccines based on recombinant viral antigens produced in plant-based expression systems are important for combating viruses. This study investigated the expression and glycosylation of different variants of the receptor-binding domain (RBD) of SARS-CoV-2 spike protein in Nicotiana benthamiana leaves. The results showed variation in yield and glycosylation characteristics among the RBD variants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Lukas Eidenberger, Florian Eminger, Alexandra Castilho, Herta Steinkellner
Summary: This study investigated the capability of three commonly used transient expression vectors to express a multi-component protein with controlled and modified N-glycosylation. The results showed that all three expression vectors produced similar quantities of targeted N-glycosylation, demonstrating the universal application of plant-based glycoengineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Zejun Mo, Wen Luo, Kai Pi, Lili Duan, Pingsong Wang, Yuzhou Ke, Shuaibo Zeng, Rongli Jia, Ting Liang, Ying Huang, Renxiang Liu
Summary: This study elucidated the importance of root uptake capacity of K+ in the formation of heterosis in tobacco leaves through comprehensive comparative transcriptome sequencing analysis. The results showed that differentially expressed genes (DEGs) exhibited dominant and over-dominant expression patterns, and genes related to metal ion transport, ion channel activity, and root development showed significantly higher expression in hybrids. These findings indicate that the over-dominant expression pattern of DEGs plays a key role in the heterosis of K+ content in tobacco leaves.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Andrea Carpi, Md Abdur Rahim, Angela Marin, Marco Armellin, Paola Brun, Giovanni Miotto, Renzo Dal Monte, Livio Trainotti
Summary: Plant cell cultures have emerged as a promising tool for producing active molecules, such as flavonols and anthocyanins, which have beneficial health properties. The synthesis of these molecules is finely regulated by specific transcription factors, and high expression levels can be achieved in tobacco cell lines. A single-column-based purification protocol was developed to produce a stable lyophile with antioxidant, anti-inflammatory, antibacterial, and wound-healing activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Qinzhi Xiao, Yueyi Zhu, Guoxian Cui, Xianwen Zhang, Risheng Hu, Zhengyu Deng, Lei Lei, Liwen Wu, Lei Mei
Summary: This study investigated the metabolic mechanism of flower color formation in tobacco by comparing the profiling of flavonoids and carotenoids in different species of tobacco. Flavonoids and carotenoids were found to contribute to the red and yellow colors of tobacco flowers, respectively.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Chemistry, Medicinal
Kuo Xu, Xinying Du, Xia Ren, XiuXue Li, Hui Li, Xianjun Fu, Xiaoyi Wei
Summary: This review comprehensively summarizes the chemical modifications and bioactivities of alpha- and beta-cembrenediols from tobacco glandular trichome secretions, highlighting their potential medicinal value for humans.
