Article
Chemistry, Medicinal
Seung-Hong Lee, Mihyang Kim, Mi Hwa Park
Summary: The study demonstrated that DPHC from Ishige okamurae has protective effects against oxidative damage in osteoblastic cells induced by H2O2 through the BMP2/Runx2 signaling pathway, improving cellular functions and promoting osteoblast differentiation. DPHC also reduced the expression levels of bone resorption-promoting factors and inhibited the generation of reactive oxygen species induced by oxidative stress.
Article
Biochemistry & Molecular Biology
Zheng Jing, Changyuan Wang, Shijie Wen, Yue Jin, Qiang Meng, Qi Liu, Jingjing Wu, Huijun Sun, Mozhen Liu
Summary: Phosphocreatine (PCr) promotes osteoblastic activities in H2O2-induced MC3T3-E1 cells by increasing osteogenic marker expression, improving OPG/RANKL ratio, and suppressing apoptosis. This effect is associated with the regulation of oxidative stress, ATP generation, and SIRT1/FOXO1/PGC-1 alpha signaling pathway.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gabrielli Collasanto Ceverino, Paula Katherine Vargas Sanchez, Roger Rodrigo Fernandes, Guilherme Alvarenga Alves, Jose Bernardo de Santis, Milla Sprone Tavares, Selma Siessere, Karina Fittipaldi Bombonato-Prado
Summary: The study demonstrated that preadministration of yerba mate extract can enhance cell adhesion, proliferation, and ALP detection of osteoblasts exposed to H2O2, while reducing the deleterious effects on cell morphology and protein expression caused by oxidative stress.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Cell Biology
Zhengtao Gu, Denghui Xie, Rui Ding, Caiqiang Huang, Yiyan Qiu
Summary: The study shows that GPR173 signaling is involved in regulating osteoblastic differentiation in MC3T3-E1 cells, with its agonist phoenixin 20 promoting the expression of osteoblast signature genes and activities. The induction by phoenixin 20 relies on the key regulator Runx2 and the activation of MAPK kinase p38. Silencing of GPR173 attenuates phoenixin 20-mediated osteoblastic differentiation, indicating its dependence on the receptor.
Article
Cell Biology
Jing Ouyang, Yaosheng Xiao, Qun Ren, Jishang Huang, Qingluo Zhou, Shanshan Zhang, Linfu Li, Weimei Shi, Zhixi Chen, Longhuo Wu
Summary: This study found that 7KC can inhibit osteogenic differentiation of MC3T3-E1 cells by inducing oxiapoptophagy. Pretreatment with the antioxidant NAC can alleviate the oxidative stress and cell apoptosis induced by 7KC, and restore the osteogenic differentiation of MC3T3-E1 cells.
Article
Biochemistry & Molecular Biology
Sagrario Martin-Aragon, Paloma Bermejo-Bescos, Juana Benedi, Carlos Raposo, Franklim Marques, Eirini K. Kydonaki, Paraskevi Gkiata, Yiannis Koutedakis, Georgia Ntina, Andres E. Carrillo, Tania Amorim
Summary: The study showed that bovine colostrum can reduce glucocorticoid-induced apoptosis in osteoblastic cells, possibly through the activation of the ERK pathway and modulation of the Hsp70 system. This provides preliminary evidence on how bovine colostrum, as a complex and multi-component dairy product, may affect osteoblastic cell survival undergoing apoptosis, in addition to its neuroprotective action.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Yu Chen, Yuwei Cai, Chunxiang Chen, Mengting Li, Lingdan Lu, Zhongxiang Yu, Shuqiang Wang, Lei Fang, Shengming Xu
Summary: This study evaluated the osteotoxicity of polychlorinated biphenyls in murine osteoblastic cells and found that oxidative stress plays a crucial role. The results showed that exposure to polychlorinated biphenyls reduced calcium homeostasis, osteoblast differentiation, and bone formation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, General & Internal
So Young Park, Kwang Sik Suh, Woon-Won Jung, Sang Ouk Chin
Summary: Spironolactone can prevent methylglyoxal-induced cellular dysfunction in MC3T3-E1 osteoblastic cells by reducing oxidative stress, improving cell survival, and enhancing bone formation activity.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2021)
Article
Cell Biology
Shan He, Hanxiang Zhang, Yang Lu, Zhaosi Zhang, Xiang Zhang, Nian Zhou, Zhenming Hu
Summary: The Nicotinamide phosphoribosyltransferase (Nampt)-NAD-Sirt1 pathway plays a role in modulating inflammation and osteogenic differentiation in MC3T3-E1 cells.
Article
Medicine, General & Internal
Pei Zhang, Jing Liao, Xiaoju Wang, Zhengping Feng
Summary: The present study compared the effects of high glucose and glucose fluctuation on apoptosis and autophagy in MC3T3-E1 osteoblasts. The results showed that high glucose and glucose fluctuation decreased cell proliferation and activated autophagy. They also induced oxidative stress, mitochondrial damage, and apoptosis, with more significant differences observed in the glucose fluctuation group. Antioxidant treatment eliminated reactive oxygen species, inhibited autophagy and apoptosis, while autophagy inhibition enhanced apoptosis in the osteoblasts.
ARCHIVES OF MEDICAL SCIENCE
(2023)
Article
Cell Biology
Magdalena Kostrzewa, Ali Mokhtar Mahmoud, Roberta Verde, Federica Scotto di Carlo, Fernando Gianfrancesco, Fabiana Piscitelli, Alessia Ligresti
Summary: Research has shown significant alterations in the endocannabinoid tone during bone cell differentiation and growth processes, affecting metabolic enzymes and endogenous ligands that directly impact bone remodeling. These findings contribute to a better understanding of bone cell physiology and offer potential therapeutic strategies for preventing bone-related metabolic changes throughout the lifespan.
Article
Biochemistry & Molecular Biology
Tong Pei, Guanyue Su, Jie Yang, Wenbo Gao, Xinrui Yang, Yaojia Zhang, Jie Ren, Yang Shen, Xiaoheng Liu
Summary: This study reveals a previously unknown relationship between FSS-induced osteogenic differentiation and autophagy, and identifies AnxA6 as a key mediator of autophagy in response to FSS. This may provide a new target for the treatment of osteoporosis and other diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Bin-Feng Cheng, Xiao Feng, Yao-Xin Gao, Shao-Qin Jian, Shi-Rao Liu, Mian Wang, Yun-Fei Xie, Lei Wang, Zhi-Wei Feng, Hai-Jie Yang
Summary: NCAM regulates osteoblast differentiation through activation of the Wnt/β-catenin and PI3K-Akt signaling pathways, with its silencing inhibiting osteoblastic differentiation in various cell lines.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Wei Yu, Chao-Ran Xie, Fan-Cheng Chen, Pei Cheng, Lei Yang, Xiao-Yun Pan
Summary: LGR5 acts as a positive regulator of osteoblastic differentiation by enhancing stability and nuclear levels of beta-catenin, leading to activation of the Wnt signaling pathway. Modulation of LGR5/Wnt/beta-catenin signaling pathway shows potential as a therapy for osteoporosis.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Medicine, General & Internal
Tian Li, Kunzhan Cai, Xiaohan Liu, Hongjuan Cao, Jie Liu, Lin Wu
Summary: This study demonstrated that inhibition of STAT3 signaling could suppress osteogenic differentiation of MC3T3-E1 cells, while forced expression of STAT3 improved osteogenic differentiation. These findings may contribute to the development of more efficient and controllable protocols for osteoblastic differentiation and provide new insights into the effect of STAT3 on osteogenesis.