Journal
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
Volume 15, Issue 12, Pages 1304-1311Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijms.25580
Keywords
cell adhesion; ITGB8; renal cell carcinoma; YB1
Categories
Funding
- National Natural Science Foundation of China [81772945, 81402094, 21575103]
- Scientific Research Foundation for the Returned Overseas Chinese scholars, Burea of personnel of China, Tianjin [2016015]
- Tianjin Natural Science Foundation [18JCYBJC26700]
- National Training Program of innovation and Entrepreneurship for undergraduates [201710062013]
- Youth Innovation Foundation of Inner Mongolia Medical University [YKD2017QNCX034]
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Background: Y-box binding protein 1 (YB1) is a multifunctional protein involved in many processes related to cancer progression and metastasis. Methods: In this study, we constructed YB1 knockdown stable renal cell carcinoma (RCC) cell line 786-0. The gene expression profile of 786-0 was performed by DNA microarray analysis to identify genes that were regulated by YB1. Real-time PCR and western blotting were used to test the genes and proteins expression. Transforming growth factor-beta (TGF-beta) activity was detected by dual-luciferase reporter assay. Cell adhesion assay was used to determine RCC cell adhesion ability. Results: Pathway analysis revealed that YB1 knockdown influenced cell adhesion molecules (CAMs). We further verified four genes (CLDN4, NRXN3, ITGB8, and VCAN) related to CAMs by real-time PCR, and confirmed that YB1 regulated the expression of ITGB8 in RCC. Functional assays demonstrated that knockdown of YB1 significantly inhibited the cell adhesion of 786-0 cells in vitro. In addition, YB1 affected TGF-beta activation. Conclusion: Our study demonstrated that YB1 modulated the adhesion ability of renal cell carcinoma cells by regulating ITGB8 and TGF-beta.
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