Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 325, Issue -, Pages 172-182Publisher
ELSEVIER
DOI: 10.1016/j.ijms.2012.06.015
Keywords
Ambient; Imaging mass spectrometry; Laser desorption; Electrospray ionization; Fungus
Funding
- National Science Council-Russian Foundation for Basic Research [99-2923-M-110-007-MY3]
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Ambient molecular imaging by Electrospray Laser Desorption/Ionization Mass Spectrometry (ELDI/MS) is described. The sample is sliced and placed onto the sample plate without further pretreatment. The distinctive distribution of the predominant chemical compounds on the surface of two dry fungi (Ganoderma lucidum and Antrodia camphorata) was obtained. Molecular images of hard and thick fungus slices were obtained for the first time, and both volatile and nonvolatile compounds originated from fungi were detected. The effects of the pulse laser frequency and the speed of the movement of the sample stage on the image quality were also investigated. (C) 2012 Elsevier B.V. All rights reserved.
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