Article
Engineering, Environmental
Pengfei Hu, Youfen Qian, Jinye Liu, Lin Gao, Yuxin Li, Yanbin Xu, Jiapeng Wu, Yiguo Hong, Tim Ford, Adi Radian, Yuchun Yang, Ji-Dong Gu
Summary: Microbial-driven nitrogen removal is crucial in full-scale wastewater treatment plants (WWTPs), and understanding the nitrogen cycling networks is essential for optimizing these processes. This study used metagenomics and metatranscriptomics to investigate the microbial nitrogen removal processes in a full-scale WWTP. The study found a simultaneous nitrification-anammox-denitrification (SNAD) process, where ammonia was oxidized by AOB, AOA, and NOB, and the resulting nitrite and nitrate were reduced to N2 by anammox and denitrifying bacteria. The study also identified specific anammox bacteria and highlighted their potential for efficient nitrogen removal.
Article
Biotechnology & Applied Microbiology
Oksana Coban, Olivia Rasigraf, Anniek E. E. de Jong, Oliver Spott, Brad M. Bebout
Summary: This study evaluated the occurrence and rates of major nitrogen cycle processes in intact microbial mats from Elkhorn Slough and Baja California Sur using N-15-labeling techniques. The results showed differences in nitrogen cycling between Elkhorn Slough and Baja mats, with differences in ammonium assimilation rates and nitrate affinity. Additionally, low nitrogen fixation rates in Elkhorn Slough mats suggest other processes contribute to nitrogen availability in these ecosystems.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Ecology
Lifei Sun, Daryl L. Moorhead, Yongxing Cui, Wolfgang Wanek, Shuailin Li, Chao Wang
Summary: This study investigated the effects of long-term exogenous nitrogen input on microbial nitrogen use efficiency (NUE) and compared EEST and 18O-labeling methods for determining NUE. The results showed that NUE estimated by EEST ranged from 0.94 to 0.98, while NUE estimated by the 18O-labeling method ranged from 0.07 to 0.30. The discrepancy in NUE values between the two methods may be due to the overestimation of microbial NUE by EEST, which did not solely focus on microbial N acquisition under exogenous nitrogen inputs.
ECOLOGICAL PROCESSES
(2023)
Review
Agricultural Engineering
Fei Han, Weizhi Zhou
Summary: Efficient nitrogen recovery from wastewater is crucial for reducing nitrogen input and mitigating nitrogen pollution, but current technologies face challenges. Utilizing microbial nitrogen assimilation shows promise for synergistic nitrogen removal and recovery in wastewater treatment.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biodiversity Conservation
Qiong Chen, Ji Chen, Mathias Neumann Andersen, Xiaoli Cheng
Summary: This study investigated the determinants of foliar-soil delta N-15 and N transportation rates at different elevations in the Hengduan Mountains. The results showed that vegetation N limitation was stronger at medium high elevations due to higher microbial immobilization and shifts in vegetation preference. Surprisingly, soil delta N-15 was not strongly correlated with foliar delta N-15, indicating that litter input may not be the main driver of soil delta N-15. Instead, soil nitrification and denitrification played a more significant role in shaping the pattern of soil delta N-15 along the elevational gradient.
GLOBAL CHANGE BIOLOGY
(2022)
Article
Plant Sciences
Kaiyan Zhang, Yanyou Wu, Yue Su, Haitao Li
Summary: The proportions of assimilated nitrate and ammonium in Brassica napus plantlets grown at different ammonium/nitrate ratios were quantified using stable nitrogen isotope tracer technique. The utilization proportions of sucrose and CO2 were quantified by a two end-member isotope mixing model. Increasing the nitrate concentration promoted the ammonium assimilation and enhanced the ammonium utilization coefficient. The amount of nitrogen derived from nitrate assimilation increased with increasing nitrate concentration, while the nitrate utilization coefficient showed no significant change.
Article
Oncology
Adam Madej, Ewa Forma, Michal Golberg, Rafal Kaminski, Piotr Paneth, Jozef Kobos, Waldemar Rozanski, Marek Lipinski
Summary: Bladder cancer is characterized by metabolic changes, resulting in differences in the abundance of natural isotopes between normal and cancerous tissues. Analysis of carbon and nitrogen isotopes in bladder cancer samples revealed lower levels compared to normal urothelium. Decreased carbon isotope abundance in normal urothelium was associated with shorter disease-free survival in bladder cancer patients. Isotope analysis can provide important information for predicting bladder cancer recurrence and improving patient management.
Article
Engineering, Environmental
Yingxue Xuan, Yingwen Mai, Yunqiu Xu, Jianyi Zheng, Zhili He, Longfei Shu, Yingjie Cao
Summary: This study investigated the major nitrogen transformations, microbial communities, and functional gene abundances in a metropolitan river network using isotope technology and bioinformatics methods. The results revealed that the bacterial community structure in highly urbanized rivers was different from that in suburban rivers, mainly due to high sewage discharge and nitrogen loadings. These changes in microbes accelerated the nitrification-denitrification processes in highly urbanized rivers.
Article
Agronomy
Pengli Yuan, Xinhao Li, Mingli Ni, Cougui Cao, Ligeng Jiang, Anas Iqbal, Jinping Wang
Summary: ORN can effectively utilize feed-N and straw-N through the compensation effect, promote the absorption and transport of nitrogen, and ultimately improve NUE in the rice-crayfish system.
Article
Soil Science
A. Watzinger, J. Prommer, A. Spiridon, W. Kisielinska, R. Hood-Nowotny, S. Leitner, W. Wanek, C. Resch, M. Heilig, E. Murer, H. Formayer, A. Wawra, J. Miloczki
Summary: Climate change scenarios predict more frequent and intense drought periods for many regions of the world, including Austria. This study simulated lower precipitation scenarios at a lysimeter station with fertile and less fertile agricultural soil. The results showed that drought significantly reduced the abundance and biodiversity of oribatid mites, while microbial communities remained unchanged. Green manure decomposition was primarily influenced by plant residue availability rather than water limitation. N2O emissions decreased and N-15 from green manure accumulated in the soil under drought conditions.
BIOLOGY AND FERTILITY OF SOILS
(2023)
Article
Microbiology
Yu Fukasawa, Koji Kaga
Summary: This study examines the effects of wood surface area on decomposition by interacting basidiomycetes using laboratory incubation experiments. The results show that wood decay rate is greater in wood with larger surface, and wood decay is accelerated by fungal interspecific interaction. The decomposition of lignin is influenced by the competitor in wood with smaller surface.
Article
Agronomy
Antong Xia, Yanyou Wu
Summary: In this study, the authors evaluated the absorption and utilization of nitrate, ammonium, and inorganic carbon during bicarbonate supply in different plant species. They found that Orychophragmus violaceus showed higher efficiency in nitrate assimilation and utilization, while higher bicarbonate levels promoted ammonium utilization and water use efficiency in both Ov and Brassica napus. However, high bicarbonate levels inhibited total inorganic carbon and nitrogen assimilation, leading to growth suppression in Bn compared to Ov. The study highlights the adaptability of Ov to karst habitats.
Article
Soil Science
Marius Schmitt, Klaus A. Jarosch, Robert Hertel, Sandra Spielvogel, Michaela A. Dippold, Sebastian Loeppmann
Summary: This study established a protocol for manufacturing multi-isotope labeled microbial necromass and successfully quantified the isotope incorporation in different microbial species. Each cultivated species showed individual patterns of tracer element uptake. The defined production of microorganism-based necromass enables versatile use in studying nutrient fluxes related to necromass in terrestrial ecosystems.
APPLIED SOIL ECOLOGY
(2022)
Article
Environmental Sciences
Eric N. Chiles, Ariana S. Huffmyer, Crawford Drury, Hollie M. Putnam, Debashish Bhattacharya, Xiaoyang Su
Summary: Corals form symbiotic relationships with dinoflagellate algae, bacteria, and other microbes, and nitrogen metabolism plays an important role in symbiotic regulation. Using stable isotope tracing, it was found that nitrogen assimilation in the coral holobiont may be compartmentalized, with different regions showing different nitrogen metabolism rates.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Geochemistry & Geophysics
Mei Yan Lai, Thomas Stachel, Richard A. Stern, Kan Li, D. Graham Pearson, Jeff W. Harris
Summary: Through examining 16 diamonds with eclogitic garnet inclusions from the Koidu kimberlite complex in Sierra Leone, it was found that the garnet inclusions have high oxygen isotope values, indicating a link to protoliths that had undergone extensive low-temperature alteration by seawater. The carbon and nitrogen isotope compositions of the host diamonds suggest that diamond formation is associated with an external, mantle-derived fluid.
GEOCHIMICA ET COSMOCHIMICA ACTA
(2023)
Article
Microbiology
Sarah Muszynski, Florian Maurer, Sina Henjes, Marcus A. Horn, Matthias Noll
Summary: The study found that natural diversity of wood decaying communities were more effective at decomposing wood compared to reduced species richness, but the latter increased wood pH. In environments with reduced species richness, there were more mutual exclusions in microbial co-occurrence networks, but microbial communities can recover previous ecosystem functions through reassembly.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Sara Fareed Mohamed Wahdan, Anna Heintz-Buschart, Chakriya Sansupa, Benjawan Tanunchai, Yu-Ting Wu, Martin Schaedler, Matthias Noll, Witoon Purahong, Francois Buscot
Summary: The relationship between active microbes and soil ecosystem functions in a grassland ecosystem is significant, with active microbes playing a crucial role in defining soil functions. The study reveals that total and active microbiomes respond differently to variations in soil physicochemical factors, with nitrogen fixing bacteria being highly active in this system. The findings suggest a stronger link between biodiversity and ecosystem functioning in soil than previously believed.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Matthias Noll, Michael Jager, Leonie Lux, Christian Buettner, Michaela Axt-Gadermann
Summary: Daily synbiotic or prebiotic baths can significantly reduce the SCORAD of AD patients over time, improve pruritus and skin dryness, and enrich the bacterial microbiome with LAB.
Article
Food Science & Technology
Marie-Louise Heymich, Laura Nissl, Dominik Hahn, Matthias Noll, Monika Pischetsrieder
Summary: The study found that antimicrobial peptides Leg1 and Leg2 from chickpea have the potential to be used as preservatives against bacterial, fungal, and oxidative spoilage in food and other products, without cytotoxic effects on human cells. Additionally, Leg2 showed antioxidative activity by radical scavenging and reducing mechanisms.
Article
Microbiology
Alice Roedel, Szilvia Vincze, Michaela Projahn, Uwe Roesler, Caroline Robe, Jens Andre Hammerl, Matthias Noll, Sascha Al Dahouk, Ralf Dieckmann
Summary: This study characterized ESBL-/AmpC-producing and NON-ESBL-/AmpC-producing E. coli isolates from German broiler farms, analyzing their genomic features and virulence profiles. While some isolates showed reduced biocide susceptibility, most biocide-tolerant isolates were rare on the tested farms. The majority of ESBL-/AmpC-producing isolates carried specific genes, indicating concerns about selection pressure for strains with acquired resistance determinants.
Article
Environmental Sciences
Lydia Kipping, Martin M. Gossner, Matthias Koschorreck, Sarah Muszynski, Florian Maurer, Wolfgang W. Weisser, Nico Jehmlich, Matthias Noll
Summary: Deadwood is a crucial part of the global carbon cycle and its decomposition releases CO2 and CH4 into the atmosphere. However, the main factors driving these greenhouse gas emissions from deadwood are not well understood. In this study, we investigated the drivers of CO2 and CH4 emission rates from deadwood specimens of different tree species exposed in forest and grassland habitats. We found that tree species identity, habitat type, management intensity, wood traits, and climate variables all play a role in determining emission rates.
GLOBAL BIOGEOCHEMICAL CYCLES
(2022)
Article
Engineering, Environmental
Sarah-Maria Scheid, Kantida Juncheed, Benjawan Tanunchai, Sara Fareed Mohamed Wahdan, Francois Buscot, Matthias Noll, Witoon Purahong
Summary: This study evaluated the influence of bio-based and biodegradable plastic PBSA on plant development and health. The results showed that PBSA and nitrogen fertilizer treatment in non-sterile soil significantly increased the disease incidence caused by F. solani on mung bean plants.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Biology
Witoon Purahong, Benjawan Tanunchai, Sarah Muszynski, Florian Maurer, Sara Fareed Mohamed Wahdan, Jonas Malter, Francois Buscot, Matthias Noll
Summary: This study investigated the role of microbial community members in wood decay by placing wood specimens in different forest sites. The findings demonstrate the interplay between fungal and bacterial communities, impacting mass loss and nitrogen content in wood. Furthermore, certain bacterial groups were found to influence the decay rates of beech wood.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alexander Kettner, Matthias Noll, Carola Griehl
Summary: In this study, the PHB formation, biomass, and elemental composition of Leptolyngbya sp. NIVA-CYA 255 were investigated. The highest PHB yields were detected in NP- and N-deficiency conditions. The glycogen pool was reduced during mixotrophy, while lipid composition was not affected. This suggests that Leptolyngbya sp. NIVA-CYA 255 is a promising candidate for PHB production.
Article
Polymer Science
Vusal Guliyev, Benjawan Tanunchai, Matthias Noll, Francois Buscot, Witoon Purahong, Evgenia Blagodatskaya
Summary: The incomplete degradation of bio-based and biodegradable plastics in soils poses threats to soil quality, human health, and food security. Bacteria show a broader functional spectrum than fungi in the degradation process of these plastics.
Article
Environmental Sciences
Christina Gross, Shakhawat Hossen, Henrik Hartmann, Matthias Noll, Werner Borken
Summary: This study investigated biological nitrogen fixation (BNF) rates and nifH gene abundances in deadwood of different tree species, and found that diffuse-porous angiosperms had higher BNF rates and nifH gene abundances. Relationships with nutrient concentrations and non-structural carbohydrates indicated that BNF in deadwood was co-limited by multiple nutrients, while high carbon availability was beneficial for diazotrophs. Water content was also found to be important for BNF and nifH gene abundance. The study suggests that BNF is a common microbial process in deadwood of native European tree species, and high nitrogen deposition does not down-regulate BNF in Central Europe.
Article
Microbiology
Angeline Hoffmann, Thomas Mueller, Volker Fingerle, Cornelia Silaghi, Matthias Noll
Summary: The study investigated the prevalence and diversity of potential human pathogenic bacteria in tick microbiomes. Rickettsia helvetica was found to be the most common species in tick microbiomes.
Article
Microbiology
Markus Thamm, Fabienne Reiss, Leon Sohl, Martin Gabel, Matthias Noll, Ricarda Scheiner
Summary: This study found that the cuticular microbiomes of solitary bees are more complex than those of social honeybees. The microbiome composition is similar among different honeybee subspecies, but subspecies adapted to warmer climates have different numbers of fungi and higher diversity. These results suggest that the cuticular microbiome of bees is influenced by visited plants, lifestyle, and climate, which is important for maintaining bee health under global change conditions.
Article
Environmental Sciences
Nadine Kiefer, Moritz Nichterlein, Fabienne Rei, Madeleine Runge, Ulf Biermann, Thomas Wieland, Matthias Noll, Stefan Kalkhof
Summary: This study examined the ecotoxicological effects of leachates containing biocides from building materials on sediment and aquatic organisms. The results showed that in-can preservatives leach out rapidly at the beginning of a facade lifetime and impose a significant stress factor on the environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Microbiology
Angeline Hoffmann, Thomas Mueller, Volker Fingerle, Matthias Noll
Summary: The study found that the presence of human pathogenic Bb affected the composition of tick microbiome, shifting the abundances of various microorganisms. Furthermore, the location had less impact on tick microbiome composition, but significantly altered the abundance of specific bacterial species and their topological roles. The regional variation in presence of Bb and other tick-associated pathogens suggests the importance of implementing a bacterial 16S rRNA gene-based microbiome survey for optimizing therapeutic approaches against Bb infection and co-occurring pathogens.
Article
Physics, Atomic, Molecular & Chemical
Daniel D. Vallejo, Joseph L. Corstvet, Facundo M. Fernandez
Summary: Electrospray ionization (ESI) is a popular method for generating ions in mass spectrometry (MS). The use of triboelectric nanogenerators as power sources for ESI shows promise in improving the analytical capabilities. This review highlights the fundamentals of ESI driven by DC power supplies, the contrasting qualities to triboelectric nanogenerator power supplies, and its applications in forensics, metabolomics, and protein structure analysis.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Florian Kluibenschedl, Anna Ploner, Christina Meisenbichler, Robert Konrat, Thomas Mueller
Summary: Mass spectrometry imaging (MSI) is a powerful analytical technique for localizing chemicals on surfaces. A new interactive approach, called interactive mass spectrometry imaging (IMSI), has been developed using a pen-like ionization interface connected to a mass spectrometer. This approach allows for real-time tracking of the pen's motion on a surface to obtain chemical data. The IMSI method was successfully tested on inkjet-printed letters and mouse brain tissue samples.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Shubhangee S. Gaikwad, Saurabh D. Kalkate, Amruta A. Bankar, Amol S. Bansode
Summary: A stable high-performance thin layer chromatographic technique was developed and validated for estimating the content of Paracetamol and Prochlorperazine. The method was validated according to the International Conference on Harmonization requirements. The study also investigated the degradation products of the drugs.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Ioan Marginean
Summary: In this study, a coherent classification of electrospray axial regimes is provided based on non-linear fluid dynamics. This classification includes periodic, transient/chaotic, and stationary regimes, and emphasizes the importance of spray current measurements and electrospray characteristic curves in diagnosing the operation and transitions between these regimes. The effect of the electrospray operating regime on the mass spectrometer signal is also summarized.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Le Thao Anh Nguyen, Chris J. Bowen, Laura Burchill, Spencer J. Williams, Richard A. J. O'Hair
Summary: D-Cysteinolic acid (D-CA) is an important metabolite in the biosulfur cycle, while a structural isomer, (R)-3-amino-2-hydroxypropanesulfonate ((R)-AHPS), is less common. Through mass spectrometry and density-functional theory, this study reveals the fragmentation reactions of these two isomers and methods to distinguish them.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
Article
Physics, Atomic, Molecular & Chemical
Frank J. Wensink, Deepak Pradeep, P. B. Armentrout, Joost M. Bakker
Summary: Methane and cyclopropane were reacted with Ru+ ions, and the resulting products were identified and characterized using mass spectrometry, IR action spectroscopy, and density functional theory calculations.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2024)
